AptaDB: Result

Interaction ID: 475

Aptamer sequence: CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCTCAT

Target unique ID: Q61727

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_470	R5A2	Protein	I10R1_MOUSE	27 nM	150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature	22434135

Aptamer Sequence:	CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCTCAT
The optimal secondary structure in dot-bracket notation:	.(((..((((.((.((.....)).)).)))))))......
The centroid secondary structure in dot-bracket notation:	..((..((((.((.((.....)).)).)))))).......

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_470	Description	R5A2
Aptamer chemistry	DNA	Length	40 nt
GC content	47.5%	Molecular weight	12,208.88 Da
Molarity of 1 μg/μl solution	81.91 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCTCAT
Applications	Bound to IL-10 receptor on the cell surface and blocked IL-10 function in vitro

Type	Detail	Type	Detail
Uniprot ID	Q61727	Protein name	Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
Gene name(s)	Il10ra Il10r	Organism	Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210)
Status	reviewed in uniprot database	Length	575
Mass	64,248	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation.

No relevant experimental diagram	Interaction ID	475
	Target type	Protein
	Target unique ID	Q61727
	Activity	27 nM
	Binding Conditions/Buffer	150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature
	Assay	N/A
	PubMed ID	22434135

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_468	DNA	CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAT	97.50%
Apta_469	DNA	CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAG	95.00%
Apta_471	DNA	CTGTAATTGGCGTATGTAACCCAGGCACCAAACGCCCCAG	92.50%
Apta_167	DNA	TGCTCCTTGGCCTTAGCCCTGGAACCATCAATCCTCTTCG	55.00%
Apta_973	DNA	TATGTAATGTTGACGTAATTTATCTCTGTGAGAATAAGCAGGTGGTATCC	54.00%
Apta_3	DNA	CCTGCCTTTGGCAGCCGGGCGAAGGCAACCCGACACCGACAGC	53.49%
Apta_987	DNA	GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC	53.19%
Apta_597	DNA	TACTGGGCTGGGCTTGGATTAGATCACAGTGGAACATACAGGACACACG	53.06%
Apta_291	DNA	AAAAATCTAGGCGCTGAAGCCCCGCTTCCTTACCTTCAC	52.50%
Apta_363	DNA	ATTGACCGCTGTGTGACGCAACACTCAAT	52.50%
Apta_789	DNA	TAACTTTGAAGGAAAGGCTACAAACTCTTC	52.50%
Apta_4	DNA	CCTGCCTTTGGAACCGACCGCCAGGCCGAGGAAAGGCACCGACAGC	52.17%
Apta_978	DNA	GGCGATGAGAACGGGAGCCTGATGATACACACGAGCGATCGCTCATAAAAGCCGTGCCCG	51.67%
Apta_571	DNA	ACCTGCGATAGGTAGTTTGTTTCTGTTGCCCTAGCGTCCGTCGTAACAAGTCA	50.94%
Apta_316	DNA	CCGCCCAAATCCCTAAGAGAAGACTGTAATGACATCAAACCAGACACACTACACACGCA	50.85%
Apta_689	DNA	CCACACAACAGCCTACGTGGATACACCACATACATCCCATAACCCCGTGTCATGTGTCG	50.85%
Apta_144	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG	50.00%
Apta_205	DNA	TTAAGGCTTTAGGTCTGAGATCTCTT	50.00%
Apta_240	DNA	TTTTTAATTAAAGCTCGCCATCAAATAGCTTT	50.00%
Apta_275	DNA	AGTTGAATGGTGTGGTCACTTCCAGTCCCGCAGGGCACAC	50.00%

ABOUT