Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 571
Aptamer sequence: CGTGTTGTCTCTCTGTGTCTCG
Target unique ID: 186907
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_539 Apt-2bp Molecule Aflatoxin B1 16 nM (1× PBS, pH 7.5) contained 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 1.75 mM KH2PO4 33052655
Structure information of aptamer
Aptamer Sequence: CGTGTTGTCTCTCTGTGTCTCG
The optimal secondary structure in dot-bracket notation: ......................
The centroid secondary structure in dot-bracket notation: ......................
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_539 Description Apt-2bp
Aptamer chemistry DNA Length 22 nt
GC content 54.5% Molecular weight 6,690.29 Da
Molarity of 1 μg/μl solution 149.47 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Aptasensor
Sequence CGTGTTGTCTCTCTGTGTCTCG
Applications Sensor for Aflflatoxin B1
Target information
Type Detail Type Detail
PubChem ID 186907 Molecular name Aflatoxin B1
Molecular formula C17H12O6 Molecular weight 312.27
IUPAC name (3S,7R)-11-methoxy-6,8,19-trioxapentacyclo[10.7.0.02,9.03,7.013,17]nonadeca-1,4,9,11,13(17)-pentaene-16,18-dione InChIKey OQIQSTLJSLGHID-WNWIJWBNSA-N
LogP 2.277 Topological polar surface area 74.97
Hydrogen bond acceptor 6 Hydrogen bond donor 0

Synonym(s):
AFLATOXIN B1; 1162-65-8; AFB1; UNII-9N2N2Y55MH; NSC-529592; 1H,11H-Cyclopenta[c]furo[3',2':4,5]furo[2,3-h][1]benzopyran-1,11-dione,2,3,6a,9a-tetrahydro-4-methoxy-, (6aR,9aS)-; AFBI; 9N2N2Y55MH; CHEBI:2504; 2,3,6aalpha,9aalpha-Tetrahydro-4-methoxycyclopenta(c)furo(3',2':4,5)furo(2,3-h)(1)benzopyran-1,11-dione; MFCD00869647; (6aR,9aS)-4-methoxy-2,3,6a,9a-tetrahydrocyclopenta[c]furo[3',2':4,5]furo[2,3-h]chromene-1,11-dione; Aflatoxin B; (3S,7R)-11-methoxy-6,8,19-trioxapentacyclo[10.7.0.02,9.03,7.013,17]nonadeca-1,4,9,11,13(17)-pentaene-16,18-dione; NSC 529592; HSDB 3453; NSC529592; CCRIS 12; Aflatoxin B1 2 microg/mL in Acetonitrile; Cyclopenta(c)furo(3',2':4,5)furo(2,3-h)(1)benzopyran-1,11-dione, 2,3,6a,9a-tetrahydro-4-methoxy-; Cyclopenta(c)furo(3',2':4,5)furo(2,3-h)(1)benzopyran-1,11-dione, 2,3,6a,9a-tetrahydro-4-methoxy-, (6aR,9aS)-; EINECS 214-603-3; BRN 1269174; Aflatoxin B1 from Aspergillus flavus, from Aspergillus flavus; (+/-)-Aflatoxin B1; BIDD:ER0313; SCHEMBL126480; Aflatoxin B1 Standard Solution; CHEMBL1697694; DTXSID9020035; Aflatoxin B1, reference material; DTXSID00873175; BDBM120261; ZINC402671; AMY22311; EX-A5480; HY-N6615; AKOS030241596; VA10187; NCGC00247669-01; 2,3,6aalpha,9aalpha-Tetrahydro-4-methoxycyclopenta(c)furo(2',3':4,5)furo(2,3-h)chromene-1,11-dione; Cyclopenta(c)furo(3',2':4,5)furo(2,3-h)(1)benzopyran-1,11-dione, 2,3,6aalpha,9aalpha-tetrahydro-4-methoxy-; CS-0034371; 162A658; Q4689278; WLN: T F5 C6 B655 DOV GV OO QO RUT&&TTJ LO1; Aflatoxin B1 solution, 20 mug/mL in methanol, analytical standard; Aflatoxin B1 solution, 2 mug/mL in acetonitrile, analytical standard; Aflatoxin B1 solution, 3 mug/mL in benzene:acetonitrile (98:2), analytical standard; Aflatoxin B1 solution, 3.79 mug/g in acetonitrile, ERM(R) certified Reference Material; Aflatoxin B1 solution, certified reference material, 20 mug/mL in methanol, ampule of 1 mL; 10279-73-9; Aflatoxin B1 solution, certified reference material, 3 mug/mL in benzene:acetonitrile (98:2), ampule of 1 mL; Cyclopenta[c]furo[3',5]furo[2,3-h][1]benzopyran-1,11-dione, 2,3,6a,9a-tetrahydro-4-methoxy-; Cyclopenta[c]furo[3',5]furo[2,3-h][1]benzopyran-1,11-dione, 2,3,6a,9a-tetrahydro-4-methoxy-, (6aR-cis)-; Cyclopenta[c]furo[3',5]furo[2,3-h][1]benzopyran-1,11-dione, 2,3,6a.alpha.,9a.alpha.-tetrahydro-4-methoxy-

Activity data
Interaction ID 571
Target type Molecule
Target unique ID 186907
Activity 16 nM
Binding Conditions/Buffer

(1× PBS, pH 7.5) contained 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 1.75 mM KH2PO4
Assay

Comparison of electrochemical Aptasensors using aptamers with different stem lengths ranging from 1 to 6 bp in responses to AFB1
PubMed ID 33052655
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_540 DNA ACGTGTTGTCTCTCTGTGTCTCGT
91.67%
Apta_538 DNA GTGTTGTCTCTCTGTGTCTC
90.91%
Apta_541 DNA CACGTGTTGTCTCTCTGTGTCTCGTG
84.62%
Apta_542 DNA GCACGTGTTGTCTCTCTGTGTCTCGTGC 78.57%
Apta_543 DNA GGCACGTGTTGTCTCTCTGTGTCTCGTGCC 73.33%
Apta_1083 DNA TGCTGTTGTCACCTGCCTCGTCTCCCTCGT 63.33%
Apta_147 DNA TGCGGTTGTAGTACTCGTGGCCG 60.87%
Apta_211 DNA GGTTGGTGTGGTTGG 54.55%
Apta_1210 DNA GGGGAGGCAGTGTGTTGTGTCGTGTGTGTGCTTGG 54.29%
Apta_203 DNA GGAAGGCTTTAGGTCTGAGATCTCGG 53.85%
Apta_350 DNA GGTGGTGGTGGTTGTGGTGGTGGTGG 53.85%
Apta_249 DNA CCCTGTTCCCCCTCCGCCGCTTGACTCTCC 53.33%
Apta_1243 DNA GGTGTTGTTGTTGGCTAGTTTGTTGTGGGC 53.33%
Apta_1247 DNA GGTGGTTGCTGTTGTTTGTGGTTGGTGTGC 53.33%
Apta_338 DNA GGGCGCGTTCTTCGTGGTTACTTTTAGTCCCG 53.12%
Apta_1263 DNA CCCCGCTGCCCGTGTTCCGTCCTCCCTTGCTGTGTGTGCG 52.50%
Apta_757 DNA GGTGGTGGTGGTTGTGGTGGTGG 52.17%
Apta_1086 DNA GCAGTTGATCCTTTGGATACCCTGG 52.00%
Apta_69 DNA AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT 51.61%
Apta_71 DNA GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC 51.61%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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