AptaDB: Result

Interaction ID: 291

Aptamer sequence: ATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT

Target unique ID: P0DJM0

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_282	A8	Protein	INLA_LISMO	N/A	TBS: Tris 10 mM, NaCl 0.85%, pH 8.0	20337767

Aptamer Sequence:	ATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT
The optimal secondary structure in dot-bracket notation:	.(((.......)))......(((..............))).......
The centroid secondary structure in dot-bracket notation:	...............................................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_282	Description	A8
Aptamer chemistry	DNA	Length	47 nt
GC content	66.0%	Molecular weight	15,051.40 Da
Molarity of 1 μg/μl solution	66.44 μM	Number of G-quadruplexes	3
G-Score	58	Function	Aptasensor
Sequence	ATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT
Applications	Biosensor for specific detection of Listeria monocytogenes

Type	Detail	Type	Detail
Uniprot ID	P0DJM0	Protein name	Internalin A
Gene name(s)	inlA lmo0433	Organism	Internalin A
Status	reviewed in uniprot database	Length	800
Mass	86,493	Activity regulation	Bacterial uptake is inhibited by EDTA and by anti-E-cadherin antibodies
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	1O6S;1O6T;1O6V;2OMT;2OMU;2OMV;2OMW;2OMX;2OMY;2OMZ;
Function	Mediates the entry of L.monocytogenes into host intestinal epithelial cells.

	Interaction ID	291
	Target type	Protein
	Target unique ID	P0DJM0
	Activity	N/A
	Binding Conditions/Buffer	TBS: Tris 10 mM, NaCl 0.85%, pH 8.0
	Assay	Analysis of interaction of aptamer A8 with protein preparations from different bacterial cultures. Proteins were vacuumimmobilized on the PVDF membrane using a slot blot and reacted with biotinylated-A8 (10 pmol L-1) and developed using streptavidin conjugated HRP (100 ng ml)-1) and a substrate. Bands marked with a dotted circle show positive reactions. Anti-InlA antibody also showed a very weak reaction with L. monocytogenes cells
	PubMed ID	20337767

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1190	DNA	TTCAGGGCGGGGTAAACGGGAGGTGGGGGGGGCTTGGGAC	61.70%
Apta_579	DNA	ACTGAGGGCACGGACAGGAGGGGGAGAGATGGCGTGAGGT	59.57%
Apta_869	DNA	ACTAGGACGCTTGGGAGGGGGGGTGGGGTGTCCGGTCGCG	59.57%
Apta_46	DNA	CGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTT	57.45%
Apta_552	DNA	CACCTGGGGGAGTATTGCGGAGGAAGGTTCCAGGTG	57.45%
Apta_590	DNA	ACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCT	57.45%
Apta_940	DNA	TGTGCGGGGGAGGGGAGTGGCGCTGTTGGTGCGGGTATAGCGCGTGGTGT	56.00%
Apta_1043	DNA	TTCAAGGCGGCGAGATTTAGTGGTTGGGAGGCTGTACGCCCTACGTGAAC	56.00%
Apta_650	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTGGGGAGGTGCGGTTGC	55.77%
Apta_488	DNA	CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG	55.32%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	55.32%
Apta_1261	DNA	GGGCGGACGCTAGGTGGTGATGCTGTGCTACACGTGTTGT	55.32%
Apta_1034	DNA	ATGCGGATCCCGCGCGCGACCGTGTCAGCGGGGACTAGCGGTGTAGCGCGAAGCTTGCGC	55.00%
Apta_794	DNA	ATCCAGAGTGACGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGACACGGTGGCTTAGT	54.79%
Apta_991	DNA	AGCAGTGAAGAGGCGAGAGATTTATGAGGGTAGCGTCCACATATGCCAGGATCGTGTTG	54.24%
Apta_1229	DNA	CACGGGCAGAGGGATAGGTTGTTGACGGGGCTGGTGGGTGGGTGCGCTCGCGCTATCGTGG	54.10%
Apta_942	DNA	AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA	54.00%
Apta_1041	DNA	TTGGCCCCGCGTGATCATTGAGGGGAGGAGCCGAGGCGGGTCCAAGATTG	54.00%
Apta_76	DNA	CAGCGCTAGGGCTTTTAGCGTAATGGGTAGGGTGGTGCGGTGCAGATATCGGAATTGGTG	53.33%
Apta_1035	DNA	ATGCGGATCCCGCGCGGGTCTGAGGAGTGCGCGGTGCCAGTGAGTGCGCGAAGCTTGCGC	53.33%

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