Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 471
Aptamer sequence: AGCTGACACAGCAGGTTGGTGGGGGTGGCTCACATCATGGTAGAATGACTGAACAGCGTTGATTAAAAGGCACGAGTCGAGCAATCTCGAAAT
Target unique ID: Q53HY0
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_466 aptamer 2 Protein CONB1_LUPAL 515 nM 10 mM Phosphate, 138 mM NaCl, 2.7 mM KCl, 1.5 mM MgCl2, pH 7.4 22529997
Structure information of aptamer
Aptamer Sequence: AGCTGACACAGCAGGTTGGTGGGGGTGGCTCACATCATGGTAGAATGACTGAACAGCGTTGATTAAAAGGCACGAGTCGAGCAATCTCGAAAT
The optimal secondary structure in dot-bracket notation: .((((...))))(((((((((((.....))))).............((((.....((.((......)).))...))))...))))))......
The centroid secondary structure in dot-bracket notation: .((((...))))...........................................((.((......)).))......................
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_466 Description aptamer 2
Aptamer chemistry DNA Length 93 nt
GC content 49.5% Molecular weight 28,950.56 Da
Molarity of 1 μg/μl solution 34.54 μM Number of G-quadruplexes 2
G-Score 21 Function Aptasensor
Sequence AGCTGACACAGCAGGTTGGTGGGGGTGGCTCACATCATGGTAGAATGACTGAACAGCGTTGATTAAAAGGCACGAGTCGAGCAATCTCGAAAT
Applications Analysis of the Lup an 1 allergen in foodstuffs
Target information
Type Detail Type Detail
Uniprot ID Q53HY0 Protein name Conglutin beta 1 (Protein Lup-1) (allergen Lup an 1) [Cleaved into: Blad]
Gene name(s) N/A Organism Conglutin beta 1 (Protein Lup-1) (allergen Lup an 1) [Cleaved into: Blad]
Status reviewed in uniprot database Length 531
Mass 62,032 Activity regulation N/A
Involvement in disease N/A Catalytic activity N/A
PDB ID(s)
N/A
Function Seed storage protein. Accumulates during seed development and is hydrolyzed after germination to provide a carbon and nitrogen source for the developing seedling.
Activity data
Interaction ID 471
Target type Protein
Target unique ID Q53HY0
Activity 515 nM
Binding Conditions/Buffer

10 mM Phosphate, 138 mM NaCl, 2.7 mM KCl, 1.5 mM MgCl2, pH 7.4
Assay

SPR Sensorgrams of the aptamer sequences obtained
PubMed ID 22529997
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_465 DNA AGCTGACACAGCAGGTTGGTGGGGGTGGCTTCCAGTTGGGTTGACAATACGTAGGGACACGAAGTCCAACCACGAGTCGAGCAATCTCGAAAT 75.27%
Apta_175 DNA AGCAGCACAGAGGTCAGATGAGGACTGTCGATAAGTATAATGGCAAGTAGCTCGATACATCCTATGCGTGCTACCGTGAA 56.99%
Apta_110 DNA AGCAGCACAGAGGTCAGATGGTTAGGAAGGTGAATAGGTACGACTGCAGGTCTATCGCAGCCTATGCGTGCTACCGTGAA 55.91%
Apta_110 DNA AGCAGCACAGAGGTCAGATGGTTAGGAAGGTGAATAGGTACGACTGCAGGTCTATCGCAGCCTATGCGTGCTACCGTGAA 55.91%
Apta_176 DNA AGCAGCACAGAGGTCAGATGTATGGTACATTCGAGCGAGCTAAACTAGGATTAAACTCACCCTATGCGTGCTACCGTGAA 55.91%
Apta_236 DNA AGCAGCACAGAGGTCAGATGAGATGGTTGACCGGTTGATGTGATTAAGGAGACGTCGATTCCTATGCGTGCTACCGTGAA 55.91%
Apta_173 DNA AGCAGCACAGAGGTCAGATGGCAGGTTTCGACGTACAATGCTATGGAGGCTTTATGATCGCCTATGCGTGCTACCGTGAA 54.84%
Apta_232 DNA AGCAGCACAGAGGTCAGATGCTATGAGCAGATCCGATAGAAAGTTAATGCCCTAGAACATCCTATGCGTGCTACCGTGAA 54.84%
Apta_535 DNA ATCCGTCACACCTGCTCTTGGGGTTATTACTCGTGAGATTGGGAATAGGTTACATGGTGTTGGCTCCCGTAT 54.84%
Apta_61 DNA AGCAGCACAGAGGTCAGATGGCAGGTAAGCAGGCGGCTCACAAAACCATTCGCATGCGGCCCTATGCGTGCTACCGTGAA 53.76%
Apta_99 DNA AGCAGCACAGAGGTCAGATGTCGGGCGGTTCGGGACCTTGCTGCGACATATGCGTATTATCCTATGCGTGCTACCGTGAA 53.76%
Apta_101 DNA AGCAGCACAGAGGTCAGATGTGTTAGGCTGGGACACGTATTACACTTACCGTAAATATTTCCTATGCGTGCTACCGTGAA 53.76%
Apta_105 DNA AGCAGCACAGAGGTCAGATGATGCTCTAGGCGAAAGACTTGGCGTAGAACGACGGGAGGCCCTATGCGTGCTACCGTGAA 53.76%
Apta_108 DNA AGCAGCACAGAGGTCAGATGCTTTGCCACGGCAACGTCCCAAAACTAATGGTAACTATACCCTATGCGTGCTACCGTGAA 53.76%
Apta_99 DNA AGCAGCACAGAGGTCAGATGTCGGGCGGTTCGGGACCTTGCTGCGACATATGCGTATTATCCTATGCGTGCTACCGTGAA 53.76%
Apta_101 DNA AGCAGCACAGAGGTCAGATGTGTTAGGCTGGGACACGTATTACACTTACCGTAAATATTTCCTATGCGTGCTACCGTGAA 53.76%
Apta_105 DNA AGCAGCACAGAGGTCAGATGATGCTCTAGGCGAAAGACTTGGCGTAGAACGACGGGAGGCCCTATGCGTGCTACCGTGAA 53.76%
Apta_108 DNA AGCAGCACAGAGGTCAGATGCTTTGCCACGGCAACGTCCCAAAACTAATGGTAACTATACCCTATGCGTGCTACCGTGAA 53.76%
Apta_178 DNA AGCAGCACAGAGGTCAGATGCGGTATGCTGGGTCATCTAACTGGGGATATTCTGATATTGCCTATGCGTGCTACCGTGAA 53.76%
Apta_102 DNA AGCAGCACAGAGGTCAGATGCGTTTTTTACGGCTGGCAACGGAAGTCAGTTTATTCTTCACCTATGCGTGCTACCGTGAA 52.69%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

HELP CENTER

>Contact us
>LMMD Group
>School of Pharmacy

DEV TOOL
Page last updated at 2022-05-25 10:01:57 (Asia/Shanghai) | You are visitor No. 43973
Copyright © 2019-2020 Laboratory of Molecular Modeling and Design, School of Pharmacy, East China University of Science and Technology. All rights reserved.