AptaDB: Result

Interaction ID: 316

Aptamer sequence: CATTCACCATGGCCCCTTCCTACGTATGTTCTGCGGGTG

Target unique ID: P14151

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_307	LD174	Protein	LYAM1_HUMAN	5.5 nM	20 mM Hepes (pH 7.5), 125 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 5 mM KCl plus 0.01% (wt/vol) human serum albumin	8981912

Aptamer Sequence:	CATTCACCATGGCCCCTTCCTACGTATGTTCTGCGGGTG
The optimal secondary structure in dot-bracket notation:	...........((((......((....)).....)))).
The centroid secondary structure in dot-bracket notation:	.......................................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_307	Description	LD174
Aptamer chemistry	DNA	Length	39 nt
GC content	56.4%	Molecular weight	12,116.55 Da
Molarity of 1 μg/μl solution	82.53 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	CATTCACCATGGCCCCTTCCTACGTATGTTCTGCGGGTG
Applications	Inhibit L-selectin binding to immobilized SLex in static assays and inhibit L-selectin-mediated rolling of human lymphocytes and neutrophils on cytokine-activated endothelial cells in flow-based assays

Type	Detail	Type	Detail
Uniprot ID	P14151	Protein name	L-selectin (CD62 antigen-like family member L) (Leukocyte adhesion molecule 1) (LAM-1) (Leukocyte surface antigen Leu-8) (Leukocyte-endothelial cell adhesion molecule 1) (LECAM1) (Lymph node homing receptor) (TQ1) (gp90-MEL) (CD antigen CD62L)
Gene name(s)	SELL LNHR LYAM1	Organism	L-selectin (CD62 antigen-like family member L) (Leukocyte adhesion molecule 1) (LAM-1) (Leukocyte surface antigen Leu-8) (Leukocyte-endothelial cell adhesion molecule 1) (LECAM1) (Lymph node homing receptor) (TQ1) (gp90-MEL) (CD antigen CD62L)
Status	reviewed in uniprot database	Length	372
Mass	42,187	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	2LGF;3CFW;5VC1;
Function	Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells.

No relevant experimental diagram	Interaction ID	316
	Target type	Protein
	Target unique ID	P14151
	Activity	5.5 nM
	Binding Conditions/Buffer	20 mM Hepes (pH 7.5), 125 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 5 mM KCl plus 0.01% (wt/vol) human serum albumin
	Assay	N/A
	PubMed ID	8981912

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1087	DNA	CTTACGACTCAGGCATTTTGCCGGGTAACGAAGTTACTGTCGTAAG	56.52%
Apta_649	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTG	56.41%
Apta_1040	DNA	CAATTGGGCCCGTCCGTATGGTGGGT	56.41%
Apta_344	DNA	CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG	54.17%
Apta_648	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGC	53.85%
Apta_1203	DNA	CCCTGCGGGGCTACCCGATATGTGTCCGAGTGGTG	53.85%
Apta_654	DNA	GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTG	53.70%
Apta_7	DNA	CCAGTCTCCCGTTTACCGCGCCTACACATGTCTGAATGCC	52.50%
Apta_57	DNA	GCGGGTCGGTTGCTCGCTTCGCCCGATCGGTCTAAGGGTG	52.50%
Apta_693	DNA	CGAGTTGAGCCGGGCGCGGTACGGGTACTGGTATGTGTGG	52.50%
Apta_1286	DNA	CTATACGTATTCTAACTTGTTACGTATTGGTCTCTTATTG	52.50%
Apta_653	DNA	CCTGCACCTCGTCCAGCATCCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGGTG	52.38%
Apta_1227	DNA	CACACCAAACACACAAGTGGACCCTGACGCATGGATAGGGTGACGGTATACGCGGGCATG	51.67%
Apta_250	DNA	CCCTGGCCGCCCTCTGGCGCGCTGATGT	51.28%
Apta_541	DNA	CACGTGTTGTCTCTCTGTGTCTCGTG	51.28%
Apta_1019	DNA	ATCCCTGCAGTTTGGTAGGGTGCAGG	51.28%
Apta_1083	DNA	TGCTGTTGTCACCTGCCTCGTCTCCCTCGT	51.28%
Apta_1192	DNA	CTTTCTGTCCTTCCGTCACATCCCACGCATTCTCCACAT	51.28%
Apta_1199	DNA	CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG	51.28%
Apta_1199	DNA	CCCTGCGGGGCTGCCCGATATGTGTCCAAGTGGTG	51.28%

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