AptaDB: Result

Interaction ID: 468

Aptamer sequence: GTTCTCATGCACACTTATAGCGGGGGGATCCAGTACT

Target unique ID: Q4W8J3

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_463	M12-23	Protein	Q4W8J3_RAT	40 nM	20 mM Hepes, pH 7.4; 50 mM NaCl; 2 mM CaCl2; 0.01% BSA	18060045

Aptamer Sequence:	GTTCTCATGCACACTTATAGCGGGGGGATCCAGTACT
The optimal secondary structure in dot-bracket notation:	((((((.(((.........))).))))))........
The centroid secondary structure in dot-bracket notation:	((((((.(((.........))).))))))........

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_463	Description	M12-23
Aptamer chemistry	DNA	Length	37 nt
GC content	51.4%	Molecular weight	11,380.32 Da
Molarity of 1 μg/μl solution	87.87 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	GTTCTCATGCACACTTATAGCGGGGGGATCCAGTACT
Applications	Costimulated T cell activation in vitro and mediated tumor rejection in mice

Type	Detail	Type	Detail
Uniprot ID	Q4W8J3	Protein name	4-1BB homolog
Gene name(s)	4-1BB	Organism	4-1BB homolog
Status	unreviewed in uniprot database	Length	258
Mass	28,297	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	N/A

	Interaction ID	468
	Target type	Protein
	Target unique ID	Q4W8J3
	Activity	40 nM
	Binding Conditions/Buffer	20 mM Hepes, pH 7.4; 50 mM NaCl; 2 mM CaCl2; 0.01% BSA
	Assay	Mice without tumors or with tumors that had not reached 12 mm diameter. Differences between the control and treatment groups were statistically significant (P<0.01)
	PubMed ID	18060045

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_751	DNA	GGTCGCAGATGGATTAAGTATGTGGGTGGGGGGGTGGAAGTTAAT	55.56%
Apta_58	DNA	GCACCTTGATGACATGATAGTCGTTGTGTATGCAGTTGGC	55.00%
Apta_1097	DNA	AGGCCCTACACGCCTTTTAGCGAGGGCGTTCCGTGGAGGA	55.00%
Apta_126	DNA	TCCTCATATAGAGTGCGGGGCGTGT	54.05%
Apta_1156	DNA	TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT	53.33%
Apta_274	DNA	GATACTGAGCATCGTACATGATCCCGCAACGGGCAGTATT	52.50%
Apta_348	DNA	ACTTGCTCTGATGCGACAACCTTTTGCCCAGATAGTAAGT	52.50%
Apta_1216	DNA	AGCGTCCTCTGCATAGTGATTTACGCTATGTGTACAGTGC	52.50%
Apta_60	DNA	GGAATGAGTGCCCTGCAAGCGAGGGCTAGC	51.35%
Apta_504	DNA	TTGCTCCTTATAGAGCCGTCCGAGC	51.35%
Apta_701	DNA	GTACTGGCGTTCGGGGGGTACCGCTGC	51.35%
Apta_717	RNA	GGCGCAGCCCGCCGAAAGGCACGUGGAACCGUC	51.35%
Apta_1019	DNA	ATCCCTGCAGTTTGGTAGGGTGCAGG	51.35%
Apta_47	DNA	GACCTAGCAGTGGACATGTGGCAGGGTGAAGTGGCATCGTC	51.22%
Apta_776	DNA	AGTGGTCGAACTACACATCCTTGAACTGCGGAATTATCTAC	51.22%
Apta_779	DNA	TACGGGCTGGATCCACTGTTACGGCGTGTATCCGCTATCAA	51.22%
Apta_744	DNA	GTGGCCAGTCACTCAATTGGGTGTAGGGGTGGGGATTGTGGGTTG	51.11%
Apta_752	DNA	GCTATCTTATGGAAATTTCGTGTAGGGTTTGGTGTGGCGGGGCTA	51.11%
Apta_819	DNA	GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT	51.11%
Apta_42	DNA	GCTGGTCTCTGCGGGTTGTTGCGCCGCGGCACCCTTGGCA	50.00%

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