AptaDB: Result

Interaction ID: 167

Aptamer sequence: GCGCATGACCATTGATGTCTGCACCATCAGAGGCAGAG

Target unique ID: P04183

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_163	TK1_apta9	Protein	KITH_HUMAN	5 nM	PBS	30900869

Aptamer Sequence:	GCGCATGACCATTGATGTCTGCACCATCAGAGGCAGAG
The optimal secondary structure in dot-bracket notation:	.....((.((.((((((.......)))))).))))...
The centroid secondary structure in dot-bracket notation:	.....((.((.((((((.......)))))).))))...

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_163	Description	TK1_apta9
Aptamer chemistry	DNA	Length	38 nt
GC content	55.3%	Molecular weight	11,952.48 Da
Molarity of 1 μg/μl solution	83.66 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	GCGCATGACCATTGATGTCTGCACCATCAGAGGCAGAG
Applications	Diagnostic assays

Type	Detail	Type	Detail
Uniprot ID	P04183	Protein name	Thymidine kinase, cytosolic (EC 2.7.1.21)
Gene name(s)	TK1	Organism	Thymidine kinase, cytosolic (EC 2.7.1.21)
Status	reviewed in uniprot database	Length	234
Mass	25,469	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=ATP + thymidine = ADP + dTMP + H(+)
PDB ID(s)	1W4R;1XBT;2ORV;2WVJ;
Function	Cell-cycle-regulated enzyme of importance in nucleotide metabolism.

No relevant experimental diagram	Interaction ID	167
	Target type	Protein
	Target unique ID	P04183
	Activity	5 nM
	Binding Conditions/Buffer	PBS
	Assay	N/A
	PubMed ID	30900869

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_162	DNA	CCGTACATACTACGTACGTACGTGAACAGAGGCAGAG	60.53%
Apta_1270	DNA	GTATATCAAGCATCGCGTGTTTACACATGCGAGAGGTGAA	57.50%
Apta_159	DNA	GCGTGCAGCATGACCACCGATGTCACACTTAATGAGAAT	56.41%
Apta_190	DNA	AGGAACCCTTTAGGTCTGAGATCTCGGAAT	55.26%
Apta_462	DNA	GGTATAGACCCCTGAGTCCTACCGAGGGCACGGCA	55.26%
Apta_917	DNA	TCGATTTCCTTAGTTGTCTTCCTTAGTGAG	55.26%
Apta_985	DNA	CCAGGAGGACCCTATTCTCGTGTATCGACGAGATCCAGTG	55.00%
Apta_1095	DNA	CCCTGAGGGGTGATGTGAATCCTAACAGTAAGGAGGAAGG	55.00%
Apta_640	DNA	CAATGGACCTATTCGAGTACTGAATAGAACAGTCGGCGCTCTGGG	53.33%
Apta_60	DNA	GGAATGAGTGCCCTGCAAGCGAGGGCTAGC	52.63%
Apta_142	DNA	CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA	52.63%
Apta_184	DNA	AGGAAGGCTTTAGGTCTGAGATCTCGGAAT	52.63%
Apta_185	DNA	ACCAAGGCTTTAGGTCTGAGATCTCCCAAT	52.63%
Apta_186	DNA	AGGAACGCTTTAGGTCTGAGATCTCGGAAT	52.63%
Apta_187	DNA	ACCAACCCTTTACCTCTGAGATCTCCCAAT	52.63%
Apta_189	DNA	AGGAACCCTTTACCTCTGAGATCTCGGAAT	52.63%
Apta_197	DNA	GGCTTTAGGTCTGAGATCTCGGAAT	52.63%
Apta_203	DNA	GGAAGGCTTTAGGTCTGAGATCTCGG	52.63%
Apta_227	DNA	ACGCGCCTATGGTGCATCGCATGCCGACC	52.63%
Apta_503	DNA	ACTTTTGAATGTGGCAACAAATTCGACAGG	52.63%

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