Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1126
Aptamer sequence: TTGAAGTGACTCCCACCCCGCCCCACCGTCCTTGCCACCGTTCCGCCATAGCAGGT
Target unique ID: O46492
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_1072 A11 Protein O46492_BOVIN 40.2 nM 150 mM NaCl, 20 mM HEPES, 1 mM KCl, 1 mM CaCl2, 1 mM MgCl2, pH 7.4 32561948
Structure information of aptamer
Aptamer Sequence: TTGAAGTGACTCCCACCCCGCCCCACCGTCCTTGCCACCGTTCCGCCATAGCAGGT
The optimal secondary structure in dot-bracket notation: .....(((.....)))..............(((((....((...))....))))).
The centroid secondary structure in dot-bracket notation: .....(((.....)))..............(((((...............))))).
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_1072 Description A11
Aptamer chemistry DNA Length 56 nt
GC content 64.3% Molecular weight 16,953.84 Da
Molarity of 1 μg/μl solution 58.98 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Target detection
Sequence TTGAAGTGACTCCCACCCCGCCCCACCGTCCTTGCCACCGTTCCGCCATAGCAGGT
Applications Detection of bPAG4 in serum samples
Target information
Type Detail Type Detail
Uniprot ID O46492 Protein name Pregnancy-associated glycoprotein 4
Gene name(s) PAG4 Organism Pregnancy-associated glycoprotein 4
Status unreviewed in uniprot database Length 380
Mass 42,663 Activity regulation N/A
Involvement in disease N/A Catalytic activity N/A
PDB ID(s)
N/A
Function N/A
Activity data
Interaction ID 1126
Target type Protein
Target unique ID O46492
Activity 40.2 nM
Binding Conditions/Buffer

150 mM NaCl, 20 mM HEPES, 1 mM KCl, 1 mM CaCl2, 1 mM MgCl2, pH 7.4
Assay

Binding curves of aptamer to bPAG4-coated MB
PubMed ID 32561948
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_1003 DNA ATAGGAGTCGACCGACCAGACCCCGCCTCTCCTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC 57.97%
Apta_997 DNA ATAGGAGTCGACCGACCAGACCCCGCCTCTCCTTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC 57.14%
Apta_1004 DNA ATAGGAGTCGACCGACCAGACCCGACCTCTCTTTCACTGTGACACAATGGTGCGTCTACATCTAGACTC 56.52%
Apta_1005 DNA ATAGGAGTCGACCGACCAGACCCGACCTCTCCTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC 56.52%
Apta_1071 DNA TTGAAGTGACTCCGCACTGGGTGGGTGGGAGGGTCGTGCGGCTGGTCATAGCAGGGT 56.14%
Apta_1007 DNA ATAGGAGTCGACCGACCAGACCCGACCTCTCTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC 55.88%
Apta_1010 DNA ATAGGAGTCGACCGACCAGACCGACCCTCGTCGTACCAACTGATGACCTAGTGGCGTCTACATCTAGACTC 54.93%
Apta_797 DNA GTTCGTGGTGTGCTGGATGTCACCTCCACTCACCACCTCTTCTCCGCTCCTCGTCATGACACATCCAGCAGCACGA 53.95%
Apta_1275 DNA CAAGTACAAACTTCCATCGCACCTCTTGCTCCCCGCGAGGGG 53.57%
Apta_1009 DNA ATAGGAGTCGACCGACCAGACCAACCCTACGCTCGTACCAACCAGATGACCTACGTGGCGTCTACATCTAGACTC 53.33%
Apta_996 DNA ATAGGAGTCGACCGACCAGACAACCCTACGCGACCAACCAGATGACCTACGTGCGTCTACATCTAGACTC 52.86%
Apta_1000 DNA ATAGGAGTCGACCGACCAGAACCGTTCCCATACTTCTCCAGCGATACTTAGTGCGTCTACATCTAGACTC 52.86%
Apta_998 DNA ATAGGAGTCGACCGACCAGACCAACCCTACGCGTACCAACCAGATGACCTACGTGCGTCTACATCTAGACTC 52.78%
Apta_796 DNA GTGCTGGATGTCACCTCCACTCACCACCTCTTCTCCGCTCCTCGTCATGACACATCCAG 52.54%
Apta_866 DNA CTCCTCTGACTGTAACCACGACCGCCTTCCACCGTTCTCCACCACCCCTCAAACAACCCTGCATAGGTAGTCCAGAAGCC 52.50%
Apta_1233 DNA TCACGAGAGAGCGAGAGCGCCCCCCCACCACAGCCGTCACCACCCTATTCCTCTGCCGTTG 52.46%
Apta_1008 DNA ATAGGAGTCGACCGACCAGACCATTCCCACGCCAAGACCAATTTTCCCTGTGCGTCTACATCTAGACTC 52.17%
Apta_1001 DNA ATAGGAGTCGACCGACCAGACAACCCTACGCGTACCAACCAGATGACCTACGTGCGTCTACATCTAGACTC 52.11%
Apta_1001 DNA ATAGGAGTCGACCGACCAGACAACCCTACGCGTACCAACCAGATGACCTACGTGCGTCTACATCTAGACTC 52.11%
Apta_863 DNA CTCCTCTGACTGTAACCACGCCCAGAAACAGCACCCTTACATCGACTGGACCTTCCGCGGCATAGGTAGTCCAGAAGCC 51.90%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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