Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 863
Aptamer sequence: TACGGGCTGGATCCACTGTTACGGCGTGTATCCGCTATCAA
Target unique ID: CRL-5800
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_779 | EJ5 | Cell | Lung Adenocarcinoma (H23) | 122 nM | 0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes | 23082117 |
Structure information of aptamer
| Aptamer Sequence: | TACGGGCTGGATCCACTGTTACGGCGTGTATCCGCTATCAA |
| The optimal secondary structure in dot-bracket notation: | ....(((.((((.(((.((....))))).)))))))..... |
| The centroid secondary structure in dot-bracket notation: | ....(((.((((.(((.((....))))).)))))))..... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_779 | Description | EJ5 |
| Aptamer chemistry | DNA | Length | 41 nt |
| GC content | 53.7% | Molecular weight | 12,592.08 Da |
| Molarity of 1 μg/μl solution | 79.41 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target detection |
| Sequence | TACGGGCTGGATCCACTGTTACGGCGTGTATCCGCTATCAA | ||
| Applications | Early detection, Targeted therapy, and guided surgery of glioblastoma leading to effective treatment of glioblastoma | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | CRL-5800 | Cell name | Lung Adenocarcinoma (H23) |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | N/A | Cell type | N/A |
| Morphology | epithelial | Tissue | Lung |
| Disease | Adenocarcinoma; Non-small cell lung cancer | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | Myc+; src+; abl+; erb+; ras+; sis- |
| Cell description | N/A |
|
|
| Application(s) | 3D cell culture; Cancer research; High-throughput screening; Toxicology | ||
Activity data
|
No relevant experimental diagram |
Interaction ID | 863 |
| Target type | Cell | |
| Target unique ID | CRL-5800 | |
| Activity | 122 nM | |
| Binding Conditions/Buffer | 0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes |
|
| Assay | N/A |
|
| PubMed ID | 23082117 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_702 | DNA | TACCCGGCGTTCGGGGGGTACCGCTGC | |
| Apta_644 | DNA | TCAATCTAACCATGCATGCAGTTTAGGCAGGATTCGTTATCGCAA | |
| Apta_883 | DNA | CGTACCGGCCAGTGATTACGACGAGACGAGCTTATGCGTATTGATGCCTAACTATCTACA | |
| Apta_1043 | DNA | TTCAAGGCGGCGAGATTTAGTGGTTGGGAGGCTGTACGCCCTACGTGAAC | |
| Apta_506 | DNA | AAGCGCGCGAGACTACGTTTGTAGGGATACCGATGTCG | |
| Apta_646 | DNA | TGGAGCTTGGATTGATGTGGTGTGTGAGTGCGGTGCCC | |
| Apta_700 | DNA | GCAGGGGCGTTCGGGGGGTACCGCTGC | |
| Apta_701 | DNA | GTACTGGCGTTCGGGGGGTACCGCTGC | |
| Apta_945 | DNA | GTTAGACGCTGGACCGCGTGTGATCGGGTCAGCTGCAATT | |
| Apta_963 | DNA | TGGCGCTGCCCCTAGTCTCCGGCTGATAGCTGCTTCTTGG | |
| Apta_985 | DNA | CCAGGAGGACCCTATTCTCGTGTATCGACGAGATCCAGTG | |
| Apta_1256 | DNA | GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACA | |
| Apta_980 | DNA | AACGAGCACGCTTTGTGTACAGCCACTAAATAAGGTATGTAGTCAGCACCCTCGTAAC | |
| Apta_845 | DNA | CCAAACGCGCTGGTTTGGAATGCTTAGAGTTTGATCGATGCCTAA | |
| Apta_1156 | DNA | TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT | |
| Apta_987 | DNA | GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC | |
| Apta_697 | DNA | CTCTCGGGACGACGGACGCTAATCTTACAAGGGCGTAGTGTATGTCGTCCC | |
| Apta_1229 | DNA | CACGGGCAGAGGGATAGGTTGTTGACGGGGCTGGTGGGTGGGTGCGCTCGCGCTATCGTGG | |
| Apta_1177 | DNA | TCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGA | |
| Apta_940 | DNA | TGTGCGGGGGAGGGGAGTGGCGCTGTTGGTGCGGGTATAGCGCGTGGTGT |
DEV TOOL
 |
 |
 |
 |