AptaDB: Result

Interaction ID: 1275

Aptamer sequence: GGACTTGAAGAAAAGGGCGACACGCGAAGTCGATGTCGCGTCTGCCTGAAGTCA

Target unique ID: P16422

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1213	Eppc14	Protein	EPCAM_HUMAN	80.77 nM	0.1 M Hepes, 0.75 M NaCl, 0.025 M KCl, 0.01 M CaCl2, 0.01 M MgCl2·6H2O in double distilled water, pH 7.4	34584404

Aptamer Sequence:	GGACTTGAAGAAAAGGGCGACACGCGAAGTCGATGTCGCGTCTGCCTGAAGTCA
The optimal secondary structure in dot-bracket notation:	.(((((..((...(((((((((((......)).)))))).)))..)).))))).
The centroid secondary structure in dot-bracket notation:	.(((((.......((.((((((...........))))))..)).....))))).

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1213	Description	Eppc14
Aptamer chemistry	DNA	Length	54 nt
GC content	55.6%	Molecular weight	16,769.77 Da
Molarity of 1 μg/μl solution	59.63 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target detection
Sequence	GGACTTGAAGAAAAGGGCGACACGCGAAGTCGATGTCGCGTCTGCCTGAAGTCA
Applications	Recognize prostate cancer cells and tissues

Type	Detail	Type	Detail
Uniprot ID	P16422	Protein name	Epithelial cell adhesion molecule (Ep-CAM) (Adenocarcinoma-associated antigen) (Cell surface glycoprotein Trop-1) (Epithelial cell surface antigen) (Epithelial glycoprotein) (EGP) (Epithelial glycoprotein 314) (EGP314) (hEGP314) (KS 1/4 antigen) (KSA) (Major gastrointestinal tumor-associated protein GA733-2) (Tumor-associated calcium signal transducer 1) (CD antigen CD326)
Gene name(s)	EPCAM GA733-2 M1S2 M4S1 MIC18 TACSTD1 TROP1	Organism	Epithelial cell adhesion molecule (Ep-CAM) (Adenocarcinoma-associated antigen) (Cell surface glycoprotein Trop-1) (Epithelial cell surface antigen) (Epithelial glycoprotein) (EGP) (Epithelial glycoprotein 314) (EGP314) (hEGP314) (KS 1/4 antigen) (KSA) (Major gastrointestinal tumor-associated protein GA733-2) (Tumor-associated calcium signal transducer 1) (CD antigen CD326)
Status	reviewed in uniprot database	Length	314
Mass	34,932	Activity regulation	N/A
Involvement in disease	Diarrhea 5, with tufting enteropathy, congenital (DIAR5)	Catalytic activity	N/A
PDB ID(s)	4MZV;6I07;
Function	May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection.

	Interaction ID	1275
	Target type	Protein
	Target unique ID	P16422
	Activity	80.77 nM
	Binding Conditions/Buffer	0.1 M Hepes, 0.75 M NaCl, 0.025 M KCl, 0.01 M CaCl2, 0.01 M MgCl2·6H2O in double distilled water, pH 7.4
	Assay	binding curves
	PubMed ID	34584404

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_686	DNA	GGTCACCAACAACAGGGAGCGCTACGCGAAGGGTCAATGTGACGTCATGCGGATGTGTGG	61.67%
Apta_910	DNA	TCACGTGCATGATAGACGGCGAAGCCGTCGAGTTGCTGTGTGCCGATGCACGTGA	60.00%
Apta_257	DNA	GGCAGAAGAAATATCGAAACCCAGAATGGTCGGCCAGGCG	55.56%
Apta_417	DNA	GGCAGGAAGACAAACACCAGGTGAGGGAGACGACGCGAGTGTTAGATGGTAGCTGTTGGTCTGTGGTGCTGT	55.56%
Apta_945	DNA	GTTAGACGCTGGACCGCGTGTGATCGGGTCAGCTGCAATT	55.56%
Apta_1220	DNA	TGGCGACAAGAAGACGTACAAACACGCACCAGGCCGGAGTGGAGTATTCTGAGGTCGG	55.17%
Apta_36	DNA	GTTAATGGGGGATCTCGCGGCCGTTCTTGTTGCTTATACA	53.70%
Apta_56	DNA	GCAGCTAAGCAGGCGGCTCACAAAACCATTCGCATGCGGC	53.70%
Apta_259	DNA	GCAAGTATGAGCGCAGGAGTTAGGTCCCGTGGCGATGGGT	53.70%
Apta_818	DNA	GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA	53.70%
Apta_876	DNA	GTTGCGAAAGACAACGAATGCTTTGCCTGCCATAATTTGC	53.70%
Apta_943	DNA	GAAGGTGGAGCGGTTAGGATACTAGCGAATGAAGACACGTTAGCTGATCT	53.70%
Apta_958	DNA	CGCGTGAGCGGGGAGGCGATGCCCAGGCTAACTTGACTCA	53.70%
Apta_965	DNA	CACGGCATGTGTGGCCGGATGCCTATATCGGTCGACTGCA	53.70%
Apta_1013	DNA	ATCACGTATGGGGGGCCGGCATGAGGGCCGCGCGTGACA	53.70%
Apta_1228	DNA	GGCCACCAAACCACACCGTCGCAACCGCGAGAACCGAAGTAGTGATCATGTCCCTGCGTG	53.33%
Apta_996	DNA	ATAGGAGTCGACCGACCAGACAACCCTACGCGACCAACCAGATGACCTACGTGCGTCTACATCTAGACTC	52.86%
Apta_998	DNA	ATAGGAGTCGACCGACCAGACCAACCCTACGCGTACCAACCAGATGACCTACGTGCGTCTACATCTAGACTC	52.78%
Apta_919	DNA	GGAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCG	52.31%
Apta_1001	DNA	ATAGGAGTCGACCGACCAGACAACCCTACGCGTACCAACCAGATGACCTACGTGCGTCTACATCTAGACTC	52.11%

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