AptaDB: Result

Interaction ID: 866

Aptamer sequence: AGGTGTGACTCGATCTGTGGGGGTTGGGGGGTGGTTTTTCGGAA

Target unique ID: TIB-202

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_782	JH19	Cell	NB4 AML cell line	7.57 nM	4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml yeast tRNA and 1 mg/ml Bovine Serum Albumin (BSA) into Phosphate buffered saline (PBS)	24405684

Aptamer Sequence:	AGGTGTGACTCGATCTGTGGGGGTTGGGGGGTGGTTTTTCGGAA
The optimal secondary structure in dot-bracket notation:	........((((((((....))))))))................
The centroid secondary structure in dot-bracket notation:	........((((((((....))))))))................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_782	Description	JH19
Aptamer chemistry	DNA	Length	44 nt
GC content	56.8%	Molecular weight	13,832.83 Da
Molarity of 1 μg/μl solution	72.29 μM	Number of G-quadruplexes	2
G-Score	20	Function	Aptasensor
Sequence	AGGTGTGACTCGATCTGTGGGGGTTGGGGGGTGGTTTTTCGGAA
Applications	Probes for acute myelogenous leukemia detection and surface protein biomarker discovery

Type	Detail	Type	Detail
ATCC ID	TIB-202	Cell name	NB4 AML cell line
Category	Human cells	Organism	Homo sapiens, human
Product type	N/A	Cell type	monocyte
Morphology	monocyte	Tissue	Peripheral blood
Disease	Acute monocytic leukemia	Product format	Frozen
Antigen expression	HLA A2, A9, B5, DRw1, DRw2	Genes expressed	Lysozyme; HLA: (A2; A9; B5; DRw2)
Cell description	THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology research, and toxicology research.
Application(s)	3D cell culture; Immune system disorder research; Immunology; Toxicology

	Interaction ID	866
	Target type	Cell
	Target unique ID	TIB-202
	Activity	7.57 nM
	Binding Conditions/Buffer	4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml yeast tRNA and 1 mg/ml Bovine Serum Albumin (BSA) into Phosphate buffered saline (PBS)
	Assay	Determination of the aptamer binding affinities to NB4 cells
	PubMed ID	24405684

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_77	DNA	GGTGTGGGCGACATGGGGGTTGGTGGTGTAGGAGCGCCAT	59.09%
Apta_941	DNA	CGGGTGCCTTAAAGTGTAGGTGGGAGGGGGATGATTATGTACGATCAAGT	58.00%
Apta_988	DNA	AAGGATGACTGACGACGATGGGAATGGAGAGGAAGGGGATATAGGTATGTTGGATGTAG	57.63%
Apta_41	DNA	CGGTCGCTCCGTGTGGCTTGGGTTGGGTGTGGCAGTGAC	56.82%
Apta_124	DNA	GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC	56.82%
Apta_675	DNA	ACTGTCCGTCGGGTTTAGGGTGGCGTTCGG	56.82%
Apta_683	DNA	GCAGTACGATCGGGGGTGGGTGGATGTAGG	56.82%
Apta_1085	DNA	TGGGGTGGTGGTGTGGGTGGGGGTTTGTTC	56.82%
Apta_1071	DNA	TTGAAGTGACTCCGCACTGGGTGGGTGGGAGGGTCGTGCGGCTGGTCATAGCAGGGT	56.14%
Apta_744	DNA	GTGGCCAGTCACTCAATTGGGTGTAGGGGTGGGGATTGTGGGTTG	55.56%
Apta_745	DNA	GGTTGCATGCCGTGGGGAGGGGGGTGGGTTTTATAGCGTACTCAG	55.56%
Apta_750	DNA	ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT	55.56%
Apta_751	DNA	GGTCGCAGATGGATTAAGTATGTGGGTGGGGGGGTGGAAGTTAAT	55.56%
Apta_46	DNA	CGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTT	54.55%
Apta_217	DNA	CGCCTGATTAGCGATACTCAGGCGTTGGGTGGGTGGGTGGG	54.55%
Apta_266	DNA	TGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCT	54.55%
Apta_393	DNA	AGGAGGATAGTTCGGTGGCTGTTCAGGGTCTCCTCCT	54.55%
Apta_481	DNA	CGGCCCACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTA	54.55%
Apta_564	DNA	GGGGACTGTTGTGGTATTATTTTTGGTTGTGCAGT	54.55%
Apta_666	DNA	TCGACCTCGCGCGAGGAGGGTGGAGGGTCGTAGAGCGCGTA	54.55%

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