AptaDB: Result

Interaction ID: 1007

Aptamer sequence: CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT

Target unique ID: Other-31

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_914	C5	Other	Rituximab	56.5 nM	20 mM Tris pH 7.6, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% v/v Tween 20	33151990

Aptamer Sequence:	CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT
The optimal secondary structure in dot-bracket notation:	..............(((..(((...)))..))).......
The centroid secondary structure in dot-bracket notation:	..............(((..(((...)))..))).......

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_914	Description	C5
Aptamer chemistry	DNA	Length	40 nt
GC content	50.0%	Molecular weight	12,183.78 Da
Molarity of 1 μg/μl solution	82.08 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target detection
Sequence	CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT
Applications	Detection of rituximab

Type	Detail	Type	Detail
Target ID	Other-31	Name	Rituximab

	Interaction ID	1007
	Target type	Other
	Target unique ID	Other-31
	Activity	56.5 nM
	Binding Conditions/Buffer	20 mM Tris pH 7.6, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% v/v Tween 20
	Assay	Titration of aptamers for heat-treated rituximab binding using ELASA
	PubMed ID	33151990

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_913	DNA	GGCACTTGTTTGCTCGAACGATGGTTTGTGCTTTTGTGTT	60.00%
Apta_1257	DNA	CGACGCACAGTCGCTGGTGCGTACCTGGTTGCCGTTGTGT	60.00%
Apta_479	DNA	CGGCCCACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTT	57.50%
Apta_480	DNA	CGGCACACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTT	57.50%
Apta_858	DNA	GGCTTCAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACG	57.50%
Apta_1241	DNA	GGTGTGGAGTGGTGTGTGTTGTTGTTTGTC	57.50%
Apta_1245	DNA	AGTGTGAAGTGGTGTGTGTTGTTGTTTGTC	57.50%
Apta_821	DNA	CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC	55.56%
Apta_216	DNA	TGGTTGGTTTTTGGTTTTTTTTTTTGGTTT	55.00%
Apta_229	DNA	GGGGCACGTTTATCCGTCCCTCCTAGTGGCGTGCCCC	55.00%
Apta_481	DNA	CGGCCCACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTA	55.00%
Apta_1027	DNA	GGAGCTACGTTTAGCGAGTCCGACGCTCGGTGCCTCTTC	55.00%
Apta_1177	DNA	TCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGA	54.35%
Apta_1278	DNA	CCATGATTCAACTTTACTGGTCTTGTCTTGGCTAGTCGTGTGTCATTCCCTAAGG	52.73%
Apta_72	DNA	AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT	52.50%
Apta_227	DNA	ACGCGCCTATGGTGCATCGCATGCCGACC	52.50%
Apta_364	DNA	TATGGTATGCTGTGTGGTATGGGGTGGCGTGCTCT	52.50%
Apta_436	DNA	CCCCCGCATCACGCCGTGGTGCGATTGACACAATTGCAAT	52.50%
Apta_482	DNA	AGCAAGGTTTGGTGTTGGTTGGTTGCTGGTTTTGGTTTC	52.50%
Apta_542	DNA	GCACGTGTTGTCTCTCTGTGTCTCGTGC	52.50%

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