Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 558
Aptamer sequence: ATACGGGAGCCAACACCAAGTAACACACGCGGACCAGAAATACATCCCCCCGTAGAGCAGGTGTGACGGAT
Target unique ID: 15558498
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_526 | AFA7 | Molecule | Aflatoxin M1 | 53.2 nM | 20 mM Tris-HCl, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2 | 24984866 |
Structure information of aptamer
| Aptamer Sequence: | ATACGGGAGCCAACACCAAGTAACACACGCGGACCAGAAATACATCCCCCCGTAGAGCAGGTGTGACGGAT |
| The optimal secondary structure in dot-bracket notation: | .(((.((........))..))).....((((.(((.....(((........))).....))).)).))... |
| The centroid secondary structure in dot-bracket notation: | .................................(......(((........)))......).......... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_526 | Description | AFA7 |
| Aptamer chemistry | DNA | Length | 71 nt |
| GC content | 54.9% | Molecular weight | 21,887.07 Da |
| Molarity of 1 μg/μl solution | 45.69 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Aptasensor |
| Sequence | ATACGGGAGCCAACACCAAGTAACACACGCGGACCAGAAATACATCCCCCCGTAGAGCAGGTGTGACGGAT | ||
| Applications | Recognition of aflflatoxin M1 | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| PubChem ID | 15558498 | Molecular name | Aflatoxin M1 |
| Molecular formula | C17H12O7 | Molecular weight | 328.27 |
| IUPAC name | (3R,7R)-3-hydroxy-11-methoxy-6,8,19-trioxapentacyclo[10.7.0.02,9.03,7.013,17]nonadeca-1,4,9,11,13(17)-pentaene-16,18-dione | InChIKey | MJBWDEQAUQTVKK-IAGOWNOFSA-N |
| LogP | 1.38 | Topological polar surface area | 95.2 |
| Hydrogen bond acceptor | 7 | Hydrogen bond donor | 1 |
Synonym(s): AFLATOXIN M1; 6795-23-9; 4-Hydroxyaflatoxin B1; UNII-I3020O28I3; I3020O28I3; (3R,7R)-3-hydroxy-11-methoxy-6,8,19-trioxapentacyclo[10.7.0.02,9.03,7.013,17]nonadeca-1,4,9,11,13(17)-pentaene-16,18-dione; (6aR,9aR)-9a-hydroxy-4-methoxy-2,3,6a,9a-tetrahydrocyclopenta[c]furo[3',2':4,5]furo[2,3-h]chromene-1,11-dione; 25325-49-9; Aflatoxin M1 0.5 microg/mL in Acetonitrile; CCRIS 15; EINECS 229-865-4; AFLATOXINM1; CHEBI:78576; DTXSID40891797; ZINC402666; Cyclopenta(c)furo(3',2':4,5)furo(2,3-h)(1)benzopyran-1,11-dione, 2,3,6a,9a-tetrahydro-9a-hydroxy-4-methoxy-; EX-A5477; MFCD00871812; Aflatoxin M1, from Aspergillus flavus; C16756; A937070; Q26998369; Aflatoxin M1 solution, 0.5 mug/mL in acetonitrile, analytical standard; (3R,7R)-3-hydroxy-11-methoxy-6,8,19-trioxapentacyclo[10.7.0.0^{2,9}.0^{3,7}.0^{13,17}]nonadeca-1,4,9,11,13(17)-pentaene-16,18-dione; (6AR-cis)-2,3,6a,9a-tetrahydro-9a-hydroxy-4-methoxycyclopenta(c)furo(3',2':4,5)furo(2,3-h)(1)benzopyran-1,11-dione; (7R)-3-Hydroxy-11-methoxy-6,8,19-trioxapentacyclo[10.7.0.02,9.03,7.013,17]nonadeca-1,4,9,11,13(17)-pentaene-16,18-dione; Aflatoxin M1 solution, certified reference material, 10 mug/mL in acetonitrile, ampule of 1 mL; Cyclopenta[c]furo[3',2':4,5]furo[2,3-h][1]benzopyran-1,11-dione,2,3,6a,9a-tetrahydro-9a-hydroxy-4-methoxy-,(6ar,9ar)-; Rel-(6aR,9aR)-9a-hydroxy-4-methoxy-2,3,6a,9a-tetrahydrocyclopenta[c]furo[3',2':4,5]furo[2,3-h]chromene-1,11-dione
Activity data
|
No relevant experimental diagram |
Interaction ID | 558 |
| Target type | Molecule | |
| Target unique ID | 15558498 | |
| Activity | 53.2 nM | |
| Binding Conditions/Buffer | 20 mM Tris-HCl, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2 |
|
| Assay | N/A |
|
| PubMed ID | 24984866 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_533 | DNA | ATACGGGAGCCAACACCAAAGTAAGATCATCACCCGGACGCGGACATAATAGGAGAGCAGGTGTGACGGAT | |
| Apta_533 | DNA | ATACGGGAGCCAACACCAAAGTAAGATCATCACCCGGACGCGGACATAATAGGAGAGCAGGTGTGACGGAT | |
| Apta_527 | DNA | ATACGGGAGCCAACACCAACAACCTAGATGTTCTGATAACACGAATCGCTTCGAAGAGCAGGTGTGACGGAT | |
| Apta_898 | DNA | ATACGGGAGCCAACACCAACAAAACTAGCAAATTAGAGCCGTATCGGAGCCTATAGAGCAGGTGTGACGGAT | |
| Apta_904 | DNA | ATACGGGAGCCAACACCAGACGGTAAATAAAGTCAGTATCGAGTACTCTATTCGAGAGCAGGTGTGACGGAT | |
| Apta_903 | DNA | ATACGGGAGCCAACACCAGACGGTAAATAAAGTCAGTATCGATACTCTATTCGAGAGCAGGTGTGACGGAT | |
| Apta_528 | DNA | ATACGGGAGCCAACACCACTTGGTTAACCAATCAAACCGGACAACGAAGGGTCCAGAGCAGGTGTGACGGAT | |
| Apta_896 | DNA | ATACGGGAGCCAACACCATAAGTCTAAAAAACTGGGAGTAAACACAATGTGTATAGAGCAGGTGTGACGGAT | |
| Apta_908 | DNA | ATACGGGAGCCAACACCAATTCCGCCAAAGTCTGCTAATAAACGGACGCCGGGGAGAGCAGGTGTGACGGAT | |
| Apta_954 | DNA | ATACGGGAGCCAACACCAGGACTAACATTATAAGAATTGCGAATAATCATTGGAGAGCAGGTGTGACGGAT | |
| Apta_824 | DNA | ATACGGGAGCCAACACCATCCCTCTTAGGATACAAAGCCAAACTGAGCCCGTGCAGAGCAGGTGTGACGGAT | |
| Apta_825 | DNA | ATACGGGAGCCAACACCACCCGTGGCCTTCACCCAGCCAGGGACCCCGTCTCTGAGAGCAGGTGTGACGGAT | |
| Apta_907 | DNA | ATACGGGAGCCAACACCACAACAAGATAAAGTATCAATCTGGTTGACGGACGTGAGAGCAGGTGTGACGGAT | |
| Apta_1048 | DNA | ATACGGGAGCCAACACCACCTGCGGAAAATCAACAACGACACACGCCACGATTCAGAGCAGGTGTGACGGAT | |
| Apta_1049 | DNA | ATACGGGAGCCAACACCATGTACATTGTAGATTTATTTGCCCAAAACCCAAGAAGAGCAGGTGTGACGGAT | |
| Apta_827 | DNA | ATACGGGAGCCAACACCACCCGTGGCCTTCACCCAGCCAGGGGCCCCGTCTCTGAGAGCAGGTGTGACGGAT | |
| Apta_901 | DNA | ATACGGGAGCCAACACCACAAGTTGACTTGGAGGAGGGTCTAACTAGTTCGAGGAGAGCAGGTGTGACGGAT | |
| Apta_897 | DNA | ATACGGGAGCCAACACCACTCTTATTTCCGGAAGTGGTTCTTCAACAGAGGAATAGAGCAGGTGTGACGGAT | |
| Apta_902 | DNA | ATACGGGAGCCAACACCACCAACATTATCAGAGTATGTTACTTATAGTGGTGGCAGAGCAGGTGTGACGGAT | |
| Apta_531 | DNA | ATACGGGAGCCAACACCACATCGACTTACGAATCAACGCGTTTATTATTGGTTCAGAGCAGGTGTGACGGAT |
DEV TOOL
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