AptaDB: Result

Interaction ID: 1049

Aptamer sequence: GGCCTGGTCTGTTAGCCGGGTAGCAGCCCCGGCACCTATT

Target unique ID: Other-40

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_966	No.1	Other	RhD+ red blood cell	580.5 nM	10 mM Tris-HCl, 50 mM KCl, pH 9.0, 1.5 mM MgCl2, 400 µM each dNTP	32319623

Aptamer Sequence:	GGCCTGGTCTGTTAGCCGGGTAGCAGCCCCGGCACCTATT
The optimal secondary structure in dot-bracket notation:	.(((.((.((((((......)))))).)).))).......
The centroid secondary structure in dot-bracket notation:	.(((.((.((((((......)))))).)).))).......

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_966	Description	No.1
Aptamer chemistry	DNA	Length	40 nt
GC content	65.0%	Molecular weight	12,280.85 Da
Molarity of 1 μg/μl solution	81.43 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	GGCCTGGTCTGTTAGCCGGGTAGCAGCCCCGGCACCTATT
Applications	For masking RhD antigens on RBCs, and thus valuable for prevention or at least amelioration of RhD+-related hemolytic anemia in RhD-individuals

Type	Detail	Type	Detail
Target ID	Other-40	Name	RhD+ red blood cell

	Interaction ID	1049
	Target type	Other
	Target unique ID	Other-40
	Activity	580.5 nM
	Binding Conditions/Buffer	10 mM Tris-HCl, 50 mM KCl, pH 9.0, 1.5 mM MgCl2, 400 µM each dNTP
	Assay	Binding dynamics of ssDNA aptamers to RhD+ RBCs. RhD+ RBCs (2% suspension, 100 μl/well) were stained in triplicate with different concentrations (200?1,200 nM) of Alexa Fluor 488?labeled each ssDNA at 37 ℃ for 60 min. After being washed, the cells were analyzed by flow cytometry. The Kd was analyzed. Data are plotted by fluorescence intensity (y?axis) against ssDNA aptamer concentrations (x-axis) using GraphPad Prism V6 software. Experiments were performed three times, and data is a mean of the triplicates. ssDNA, single-stranded DNA; Rh, Rhesus; RBCs, red blood cells; Kd, dissociation constant
	PubMed ID	32319623

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_42	DNA	GCTGGTCTCTGCGGGTTGTTGCGCCGCGGCACCCTTGGCA	57.50%
Apta_1018	DNA	ATCCCTGCAGTTTGGTAGGGTGCGAGCAACATCAGGCACCTGCAGG	56.52%
Apta_3	DNA	CCTGCCTTTGGCAGCCGGGCGAAGGCAACCCGACACCGACAGC	55.81%
Apta_255	DNA	TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT	53.33%
Apta_818	DNA	GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA	53.33%
Apta_820	DNA	TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA	53.33%
Apta_192	DNA	AGGAAGGCTTTAGCTCTGAGATCTCGGAAT	52.50%
Apta_195	DNA	AGGAAGGCTTTAGGGGTCTGAGATCTCGGAAT	52.50%
Apta_264	DNA	GGCGTTGTCGGGCGCAGGTGTAGGCCTCGTGGTGGTGGGT	52.50%
Apta_443	DNA	GGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA	52.50%
Apta_857	DNA	GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC	52.50%
Apta_915	DNA	ACTTCGGCTAGTTAGGGGGTAGTTTAGATCGTCTCTACAT	52.50%
Apta_916	DNA	GGCGTATGCAGTTTTGTAGGGTTATCAAGGCTTGACGATT	52.50%
Apta_1027	DNA	GGAGCTACGTTTAGCGAGTCCGACGCTCGGTGCCTCTTC	52.50%
Apta_1083	DNA	TGCTGTTGTCACCTGCCTCGTCTCCCTCGT	52.50%
Apta_943	DNA	GAAGGTGGAGCGGTTAGGATACTAGCGAATGAAGACACGTTAGCTGATCT	52.00%
Apta_1041	DNA	TTGGCCCCGCGTGATCATTGAGGGGAGGAGCCGAGGCGGGTCCAAGATTG	52.00%
Apta_1045	DNA	GGTGCTCAGGGAACTGTCTGAGGGATCAGGCTTAAGCCTGTCGAGCAGTT	52.00%
Apta_1156	DNA	TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT	51.11%
Apta_282	DNA	ATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT	51.06%

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