AptaDB: Result

Interaction ID: 527

Aptamer sequence: CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG

Target unique ID: CRM-HTB-26

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_344	SYL3C	Cell	MDA-MB-231 cells	38 nM	1 x PBS (pH-7.4) and 5 mM MgCl2	23480100

Aptamer Sequence:	CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG
The optimal secondary structure in dot-bracket notation:	....(((((.......)))))((((........))))(((....))).
The centroid secondary structure in dot-bracket notation:	........((((.........((((........))))......)))).

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_344	Description	SYL3C
Aptamer chemistry	DNA	Length	48 nt
GC content	60.4%	Molecular weight	15,078.41 Da
Molarity of 1 μg/μl solution	66.32 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG
Applications	Binding to targer cell

Type	Detail	Type	Detail
ATCC ID	CRM-HTB-26	Cell name	MDA-MB-231 cells
Category	Human cells	Organism	Homo sapiens, human
Product type	Certified reference material	Cell type	epithelial cell
Morphology	epithelial	Tissue	Breast; Mammary gland
Disease	Adenocarcinoma	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	N/A
Application(s)	Assay development

	Interaction ID	526
	Target type	Cell
	Target unique ID	HTB-103
	Activity	67 nM
	Binding Conditions/Buffer	1 x PBS (pH-7.4) and 5 mM MgCl2
	Assay	Binding affinity determination (Kd) of SYL3 aptamer
	PubMed ID	23480100
	Interaction ID	527
	Target type	Cell
	Target unique ID	CRM-HTB-26
	Activity	38 nM
	Binding Conditions/Buffer	1 x PBS (pH-7.4) and 5 mM MgCl2
	Assay	Binding affinity determination (Kd) of SYL3 aptamer
	PubMed ID	23480100

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_577	DNA	ACTTCAGTGAGTTGTCCCACGGTCGGCGAGTCGGTGGTAG	60.42%
Apta_649	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTG	60.42%
Apta_654	DNA	GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTG	59.26%
Apta_148	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGGTGGCGTGG	56.25%
Apta_578	DNA	CACCAAGCGCAGGGAATTACATTGAAGTGTGGGATTGGCT	56.25%
Apta_648	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGC	56.25%
Apta_1262	DNA	ACAAGGGGTGCAGGGGTCCGCGATTGTGACTGTGTGTGCG	56.25%
Apta_1267	DNA	GGCACGGCAAAGGGGTACAGCCTACCGAACCGTGGCTGTAAGGGTGGTTGTGGTGTG	56.14%
Apta_595	DNA	AGCGAGGGCGGTGTCCAACAGCGGTTTTTTCACGAGGAGGTTGGCGGTGG	56.00%
Apta_650	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTGGGGAGGTGCGGTTGC	55.77%
Apta_223	DNA	CATTGAGATAGCTAGTTGTAGCTGCGTCATAGGCTGGGTTGGGTCTAGTGGTTGGGTGTG	55.00%
Apta_652	DNA	CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGGCAGGGGGTGTGCGTG	54.55%
Apta_72	DNA	AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT	54.17%
Apta_149	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGATGGCGTGG	54.17%
Apta_150	DNA	CCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGG	54.17%
Apta_217	DNA	CGCCTGATTAGCGATACTCAGGCGTTGGGTGGGTGGGTGGG	54.17%
Apta_307	DNA	CATTCACCATGGCCCCTTCCTACGTATGTTCTGCGGGTG	54.17%
Apta_474	DNA	CAAAAAGATAGGTGGATTTTTCCCCGGATCGGTTTGTATG	54.17%
Apta_475	DNA	CAAAAAGATAGGTGGATTTTTCCCCGGGTCGGTTTGTATG	54.17%
Apta_478	DNA	CTTCACTTTCGTGGACGGTGGCGTCGGGGCGGTTTGTATG	54.17%

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