AptaDB: Result

Interaction ID: 907

Aptamer sequence: CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC

Target unique ID: 35556

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_821	SA34	Cell	Staphylococcus aureus	72.42 nM	0.1 mg/ml salmon sperm DNA (0.1 mg/ml tRNA, 1% BSA, 1% PBS, 0.05% (v/v) Tween-20	19498077

Aptamer Sequence:	CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC
The optimal secondary structure in dot-bracket notation:	....(((.....)))(((.((....)).))).....(((...)))
The centroid secondary structure in dot-bracket notation:	....(((.....)))(((.((....)).))).....(((...)))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_821	Description	SA34
Aptamer chemistry	DNA	Length	45 nt
GC content	55.6%	Molecular weight	13,763.82 Da
Molarity of 1 μg/μl solution	72.65 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target detection
Sequence	CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC
Applications	Detection of Staphylococcus aureus

Type	Detail	Type	Detail
ATCC ID	35556	Cell name	Staphylococcus aureus
Category	Bacteria	Organism	N/A
Product type	N/A	Cell type	N/A
Morphology	N/A	Tissue	N/A
Disease	N/A	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	Restriction-deficient mutant derived from strain NCTC 8325.
Application(s)	Bioinformatics; Molecular biology

	Interaction ID	907
	Target type	Cell
	Target unique ID	35556
	Activity	72.42 nM
	Binding Conditions/Buffer	0.1 mg/ml salmon sperm DNA (0.1 mg/ml tRNA, 1% BSA, 1% PBS, 0.05% (v/v) Tween-20
	Assay	Flow cytometry assay for the binding of aptamers with bacteria
	PubMed ID	19498077

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1257	DNA	CGACGCACAGTCGCTGGTGCGTACCTGGTTGCCGTTGTGT	57.78%
Apta_158	DNA	CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTG	55.56%
Apta_183	DNA	TAGCACTGTGCGGCGCGTATTGTTCTAATCTGATTACATT	55.56%
Apta_339	DNA	CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTGT	55.56%
Apta_437	DNA	TCACAGTCCCGGTGCCGCACTAAAACCCATTGTTGTGCGA	55.56%
Apta_673	DNA	GCAGTCCTAGATCGGGTGTGGCTGGCTTGG	55.56%
Apta_692	DNA	ACGTTGACGCTGGTGCCCGCTTGTGGTGCGAGTGTTGTGT	55.56%
Apta_733	DNA	TAGGCAGTGGTTTGACGTCCGCATGTTGGGAATAGCCACGCCT	55.56%
Apta_914	DNA	CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT	55.56%
Apta_357	DNA	ACAATGTCGCTCTCCGCCGCAGGAGCATTGTCTGTCTTTATGCTTCTCTTTTTTGTTGC	54.24%
Apta_238	DNA	CACACATTCCCGTCCTCGATACGTCTAGGCTTAGTGCCACTTGCTTAATC	54.00%
Apta_882	DNA	AATAGTCTGGTTCGGTGCTTGGTGCGATGTGTTAGTCAGACTCTTGTGTG	54.00%
Apta_58	DNA	GCACCTTGATGACATGATAGTCGTTGTGTATGCAGTTGGC	53.33%
Apta_259	DNA	GCAAGTATGAGCGCAGGAGTTAGGTCCCGTGGCGATGGGT	53.33%
Apta_406	DNA	AAAGCCCACTGCATAGAGGCCGCTCAACATGCGAGCCGGT	53.33%
Apta_480	DNA	CGGCACACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTT	53.33%
Apta_506	DNA	AAGCGCGCGAGACTACGTTTGTAGGGATACCGATGTCG	53.33%
Apta_648	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGC	53.33%
Apta_815	DNA	CATCCGTCACACCTGCTCTGGCCACTAACATGGGGACCAGGTGGTGTTGGCTCCCGTATC	53.33%
Apta_817	DNA	TGCGTGAGCGGTAGGCCCCTACGACCCACTGTGGTTGGGC	53.33%

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