AptaDB: Result

Interaction ID: 904

Aptamer sequence: GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA

Target unique ID: 35556

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_818	SA23	Cell	Staphylococcus aureus	61.5 nM	0.1 mg/ml salmon sperm DNA (0.1 mg/ml tRNA, 1% BSA, 1% PBS, 0.05% (v/v) Tween-20	19498077

Aptamer Sequence:	GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA
The optimal secondary structure in dot-bracket notation:	(((((((.....)))).))).((((....))))............
The centroid secondary structure in dot-bracket notation:	(((((((.....)))).))).((((....))))............

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_818	Description	SA23
Aptamer chemistry	DNA	Length	45 nt
GC content	57.8%	Molecular weight	13,855.90 Da
Molarity of 1 μg/μl solution	72.17 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target detection
Sequence	GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA
Applications	Detection of Staphylococcus aureus

Type	Detail	Type	Detail
ATCC ID	35556	Cell name	Staphylococcus aureus
Category	Bacteria	Organism	N/A
Product type	N/A	Cell type	N/A
Morphology	N/A	Tissue	N/A
Disease	N/A	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	Restriction-deficient mutant derived from strain NCTC 8325.
Application(s)	Bioinformatics; Molecular biology

	Interaction ID	904
	Target type	Cell
	Target unique ID	35556
	Activity	61.5 nM
	Binding Conditions/Buffer	0.1 mg/ml salmon sperm DNA (0.1 mg/ml tRNA, 1% BSA, 1% PBS, 0.05% (v/v) Tween-20
	Assay	Flow cytometry assay for the binding of aptamers with bacteria
	PubMed ID	19498077

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_159	DNA	GCGTGCAGCATGACCACCGATGTCACACTTAATGAGAAT	62.22%
Apta_48	DNA	ACTGGGCAGATTAACGGTCCTATATTTTCGGTTCTGGAAA	55.56%
Apta_161	DNA	GTGTGCAGATCTGATTGCCTGCGTCAACCTACATACAAC	55.56%
Apta_967	DNA	GGGTAGCAGCCCCGCGGAGGGTCGGCTATAAGAACCAAGA	55.56%
Apta_1213	DNA	GGACTTGAAGAAAAGGGCGACACGCGAAGTCGATGTCGCGTCTGCCTGAAGTCA	53.70%
Apta_146	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCG	53.33%
Apta_462	DNA	GGTATAGACCCCTGAGTCCTACCGAGGGCACGGCA	53.33%
Apta_879	DNA	GGGGGGGTGAGACATCGGGCATGTTCATGGGTGGGGGGTA	53.33%
Apta_966	DNA	GGCCTGGTCTGTTAGCCGGGTAGCAGCCCCGGCACCTATT	53.33%
Apta_597	DNA	TACTGGGCTGGGCTTGGATTAGATCACAGTGGAACATACAGGACACACG	53.06%
Apta_796	DNA	GTGCTGGATGTCACCTCCACTCACCACCTCTTCTCCGCTCCTCGTCATGACACATCCAG	52.54%
Apta_558	DNA	CCGGTGGGTGGTCAGGTGGGATAGCGTTCCGCGTATGGCCCAGCGCATCACGGGTTCGCACCA	52.38%
Apta_684	DNA	GGCGGACCAAGGGGACACCACAGATGAATGTACAGTACCATGTTACTGCGCCCGTAGGTG	51.67%
Apta_883	DNA	CGTACCGGCCAGTGATTACGACGAGACGAGCTTATGCGTATTGATGCCTAACTATCTACA	51.67%
Apta_993	DNA	AATCCGACCACGAGAGCAGACCGACCACAGCATTATCTAAACCCATTAGTCTAGTCCTGG	51.67%
Apta_77	DNA	GGTGTGGGCGACATGGGGGTTGGTGGTGTAGGAGCGCCAT	51.11%
Apta_165	DNA	TGGACGAACTGCCCTCAGCTACTTTCATGTTGCTGACGCA	51.11%
Apta_221	DNA	GTCCTAGCAGACACTCGGCCATCACGCCCATTGGTTTGCA	51.11%
Apta_338	DNA	GGGCGCGTTCTTCGTGGTTACTTTTAGTCCCG	51.11%
Apta_602	DNA	GGGCGAGAACAGTGTCCCAGTCGCTAGTTTTCTCTGCCC	51.11%

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