Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 824
Aptamer sequence: ATCGTCTGCTCCGTCCAATAGGACCGATAGGAACCACGGACTGCATGTTTCTGCATTTGACGTGGTTTGGTGTGAGGTCGTGC
Target unique ID: CRM-CCL-185
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_742 TV06 Cell vaccinia virus–infected and–uninfected lung cancer A549 cells 6.5 nM Dulbecco PBS, 4.5g/L glucose, 5 mmol/L MgCl2, 0.1 g/L yeast tRNA, 100 mL/L FBS, and 1 g/L BSA 19246617
Structure information of aptamer
Aptamer Sequence: ATCGTCTGCTCCGTCCAATAGGACCGATAGGAACCACGGACTGCATGTTTCTGCATTTGACGTGGTTTGGTGTGAGGTCGTGC
The optimal secondary structure in dot-bracket notation: ..(((((((.((((((....))))......((((((((...((((......)))).....)))))))))).)).))).))...
The centroid secondary structure in dot-bracket notation: ............((((....))))......((((((((...((((......)))).....))))))))...............
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_742 Description TV06
Aptamer chemistry DNA Length 83 nt
GC content 53.0% Molecular weight 25,661.38 Da
Molarity of 1 μg/μl solution 38.97 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Target recognition
Sequence ATCGTCTGCTCCGTCCAATAGGACCGATAGGAACCACGGACTGCATGTTTCTGCATTTGACGTGGTTTGGTGTGAGGTCGTGC
Applications Detection of A549 cells
Target information
Type Detail Type Detail
ATCC ID CRM-CCL-185 Cell name vaccinia virus-infected and-uninfected lung cancer A549 cells
Category Human cells Organism Homo sapiens, human
Product type Certified reference material Cell type epithelial cell
Morphology epithelial Tissue Lung
Disease Carcinoma Product format Frozen
Antigen expression N/A Genes expressed N/A
Cell description N/A
Application(s) Assay development
Activity data

No relevant experimental diagram

 Interaction ID 824
Target type Cell
Target unique ID CRM-CCL-185
Activity 6.5 nM
Binding Conditions/Buffer

Dulbecco PBS, 4.5g/L glucose, 5 mmol/L MgCl2, 0.1 g/L yeast tRNA, 100 mL/L FBS, and 1 g/L BSA
Assay

N/A
PubMed ID 19246617
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_743 DNA ATCGTCTGCTCCGTCCAATATCCGAGCAAGAACTCATATTGCATTATTTATAGCTACGCGCTGCATTTGGTGTGAGGTCGTGC 73.49%
Apta_738 DNA ATCGTCTGCTCCGTCCAATACCTGCATATACACTTTGCATGTGGTTTGGTGTGAGGTCGTGC 71.08%
Apta_737 DNA ATCGTCTGCTCCGTCCAATAGTGCATTGAAACTTCTGCATCCTCGTTTGGTGTGAGGTCGTGC 69.88%
Apta_739 DNA ATCGTCTGCTCCGTCCAATAGCGTGCATTGGTTTACTGCATCCGTGAAACTGGGCTTTGGTGTGAGGTCGTGC 69.88%
Apta_736 DNA ATCGTCTGCTCCGTCCAATATGATGACACCTGCATAATTTATAGTGAGTCTTGATTCACGCTGCATTTGGTGTGAGGTCGTGC 68.67%
Apta_740 DNA ATCGTCTGCTCCGTCCAATAAACCTGCATAATTTATAAGTCTAGACTGCTGCATTTGGTGTGAGGTCGTGC 68.67%
Apta_741 DNA ATCGTCTGCTCCGTCCAATAGCCTCACCCTGCATAATTTATAGACTACACTTAGGAATCGCTGCATTTGGTGTGAGGTCGTGC 67.47%
Apta_791 DNA ATCGTCTGCTCCGTCCAATATATCAAAGGCGAATTTTGTCAAGGTGTTAAACGATAGTCCCTACCTTTGGTGTGAGGTCGTGC 65.06%
Apta_1231 DNA ACCACCGGCCCGAGATAGTCTAGACCACTATGTTGTTGTGCTTACTGCTGTGTGGTGTGG 57.83%
Apta_1050 DNA ATCCGTCACACCTGCTCTCACGTCCGTCAACCAGATTGATACTTTATCTTGTTGTGGTGTTGGCTCCCGTAT 55.42%
Apta_657 DNA CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC 54.55%
Apta_79 DNA ATCTCGAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACTCGT 54.22%
Apta_530 DNA ATCCGTCACACCTGCTCTGTGTACGCCCGTATTTACGTTCCTAGCAATTGCTATGTGGTGTTGGCTCCCGTAT 54.22%
Apta_536 DNA ATCCGTCACACCTGCTCTCGAATAGAGTACTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT 54.22%
Apta_651 DNA CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTG 54.22%
Apta_1220 DNA TGGCGACAAGAAGACGTACAAACACGCACCAGGCCGGAGTGGAGTATTCTGAGGTCGG 54.22%
Apta_336 DNA CATGCTTCCCCAGGGAGATGACCGGGGCGTACACCGTCGCGGCACATGTCTGAATGCGTTTAGTCTCTGTGG 53.01%
Apta_357 DNA ACAATGTCGCTCTCCGCCGCAGGAGCATTGTCTGTCTTTATGCTTCTCTTTTTTGTTGC 53.01%
Apta_524 DNA ATCCGTCACACCTGCTCTGGCATTAGTATTCCATAGCCGGCCAAGTCTATGTAGTGGTGTTGGCTCCCGTAT 53.01%
Apta_525 DNA ATCCGTCACACCTGCTCTCAATCTGAAATATTGCAAGCAGTGCTCACAATTTGTTGGTGTTGGCTCCCGTAT 53.01%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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