AptaDB: Result

Interaction ID: 827

Aptamer sequence: GGTTGCATGCCGTGGGGAGGGGGGTGGGTTTTATAGCGTACTCAG

Target unique ID: CRM-CCL-185

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_745	S1	Cell	vaccinia virus–infected and–uninfected lung cancer A549 cells	40.9 nM	5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium	19684903

Aptamer Sequence:	GGTTGCATGCCGTGGGGAGGGGGGTGGGTTTTATAGCGTACTCAG
The optimal secondary structure in dot-bracket notation:	(((.....)))..........(((((.(((....))).)))))..
The centroid secondary structure in dot-bracket notation:	(((.....)))..........(((((.(((....))).)))))..

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_745	Description	S1
Aptamer chemistry	DNA	Length	45 nt
GC content	60.0%	Molecular weight	14,116.01 Da
Molarity of 1 μg/μl solution	70.84 μM	Number of G-quadruplexes	3
G-Score	63	Function	Target recognition
Sequence	GGTTGCATGCCGTGGGGAGGGGGGTGGGTTTTATAGCGTACTCAG
Applications	Recognition of subtype non-small cell lung cancer

Type	Detail	Type	Detail
ATCC ID	CRM-CCL-185	Cell name	vaccinia virus-infected and-uninfected lung cancer A549 cells
Category	Human cells	Organism	Homo sapiens, human
Product type	Certified reference material	Cell type	epithelial cell
Morphology	epithelial	Tissue	Lung
Disease	Carcinoma	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	N/A
Application(s)	Assay development

	Interaction ID	827
	Target type	Cell
	Target unique ID	CRM-CCL-185
	Activity	40.9 nM
	Binding Conditions/Buffer	5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco's PBS without calcium and magnesium
	Assay	The binding of selected aptamers to A549 cells pre-treated with trypsin
	PubMed ID	19684903

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_869	DNA	ACTAGGACGCTTGGGAGGGGGGGTGGGGTGTCCGGTCGCG	60.00%
Apta_41	DNA	CGGTCGCTCCGTGTGGCTTGGGTTGGGTGTGGCAGTGAC	57.78%
Apta_77	DNA	GGTGTGGGCGACATGGGGGTTGGTGGTGTAGGAGCGCCAT	57.78%
Apta_666	DNA	TCGACCTCGCGCGAGGAGGGTGGAGGGTCGTAGAGCGCGTA	57.78%
Apta_870	DNA	CCGGGGAATCTAGGGGAGGGCGGGTGGGTCAATTGAGCCG	57.78%
Apta_967	DNA	GGGTAGCAGCCCCGCGGAGGGTCGGCTATAAGAACCAAGA	57.78%
Apta_1082	DNA	GGCTGGTCCGCTGGGAACAAGGGCGGGAGGGAGGGTGTGGGTGCGACAAGCGGACCAGCC	56.67%
Apta_795	DNA	CGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGA	56.25%
Apta_941	DNA	CGGGTGCCTTAAAGTGTAGGTGGGAGGGGGATGATTATGTACGATCAAGT	56.00%
Apta_618	DNA	GCGGCAAATCAGCATACGAGTGTACAGGGACGGGACGGGTGGGTAAAAGGTGTCGCCTCAG	55.74%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	55.56%
Apta_782	DNA	AGGTGTGACTCGATCTGTGGGGGTTGGGGGGTGGTTTTTCGGAA	55.56%
Apta_862	DNA	TGTGCCAAAGAGAGTGGTGGGGGGGTGGGCGGAACTCGCG	55.56%
Apta_915	DNA	ACTTCGGCTAGTTAGGGGGTAGTTTAGATCGTCTCTACAT	55.56%
Apta_1255	DNA	GGCGCATGATCATTCGGTGGGTAAGGTGGTGGTAACGTTG	55.56%
Apta_1081	DNA	CTTACGACTGTGGTCGGGTGGTGGGCCTCTAGAGGGGTGTCGTAAG	54.35%
Apta_988	DNA	AAGGATGACTGACGACGATGGGAATGGAGAGGAAGGGGATATAGGTATGTTGGATGTAG	54.24%
Apta_756	DNA	GGGGGACACGGAGGTGGTGGAAAGGCTAAGATTTGATGATGAGTAGTGTGGT	53.85%
Apta_46	DNA	CGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTT	53.33%
Apta_124	DNA	GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC	53.33%

ABOUT