Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 896
Aptamer sequence: ACAGCATCCCCATGTGAACAATCGCATTGTGATTGTTACGGTTTCCGCCTCATGGACGTGCTG
Target unique ID: PTA-7435
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_810 TLS11a Cell Human Hepatocellular carcinoma cell line (LH86) 7.16 nM PBS containing 5 mM MgCl2, 4.5 mg/mL glucose, 0.1 mg/mL yeast tRNA, 1 mg/mL BSA 22558072
Structure information of aptamer
Aptamer Sequence: ACAGCATCCCCATGTGAACAATCGCATTGTGATTGTTACGGTTTCCGCCTCATGGACGTGCTG
The optimal secondary structure in dot-bracket notation: .((((((..(((((..(((((((((...)))))))))..(((....))).)))))..))))))
The centroid secondary structure in dot-bracket notation: .((((((..(((((..(((((((((...)))))))))..(((....))).)))))..))))))
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_810 Description TLS11a
Aptamer chemistry DNA Length 63 nt
GC content 50.8% Molecular weight 19,339.38 Da
Molarity of 1 μg/μl solution 51.71 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted therapy
Sequence ACAGCATCCCCATGTGAACAATCGCATTGTGATTGTTACGGTTTCCGCCTCATGGACGTGCTG
Applications Targeted Delivery
Target information
Type Detail Type Detail
ATCC ID PTA-7435 Cell name Human Hepatocellular carcinoma cell line (LH86)
Category N/A Organism N/A
Product type N/A Cell type N/A
Morphology N/A Tissue N/A
Disease N/A Product format Frozen
Antigen expression N/A Genes expressed N/A
Cell description N/A
Application(s) N/A
Activity data
Interaction ID 896
Target type Cell
Target unique ID PTA-7435
Activity 7.16 nM
Binding Conditions/Buffer

PBS containing 5 mM MgCl2, 4.5 mg/mL glucose, 0.1 mg/mL yeast tRNA, 1 mg/mL BSA
Assay

Kd determination
PubMed ID 22558072
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_451 DNA ATACCAGCTTATTCAATTGAGTAAGAGTGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCAATCT 55.26%
Apta_1089 DNA ATCCAGAGTGACGCAGCAATTACCGATGGCTTGTTTGTAATGTAGGGTTCCGTCGGATTGGACACGGTGGCTTAGT 55.26%
Apta_655 DNA CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTG 54.79%
Apta_587 DNA GGAATGGATCCACATCCATGGATGGGCAATGCGGGGTGGAGAATGGTTGCCGCACTTCGGCTTCACTGCAGACTTGACGAAGCTT 54.12%
Apta_1177 DNA TCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGA 53.97%
Apta_233 DNA AGCAGCACAGAGGTCAGATGTGAAACATAGCATATTTACTTATGTCGCCTTGCCGGTTCCTATGCGTGCTACCGTGAA 53.85%
Apta_887 DNA ACGCTCGGATGCCACTACAGGGGGGTGGTCCTGAGGGTGGTGTGGTTGGTTTGGTTTCCTCATGGACGTGCTGGTGAC 53.85%
Apta_1119 DNA GGGAATGGATCCACATCTACGAATTCGGTTGTGAAGATTGGGAGCGTCGTGGCTACTTCACTGCAGACTTGACGAAGCTT 53.75%
Apta_1178 DNA AGCGTCGAATACCACTACAGGGGATGCGAAGTTCCGCGGTCGAGTATATGATACATCCATCTAATGGAGCTCGTGGTCAG 53.75%
Apta_725 DNA ATCCAGAGTGACGCAGCATGCTCATCCACGATTCTGGCGAATTTAGTGCCTGTCTTTTTCTCTTGGACACGGTGGCTTAGT 53.09%
Apta_736 DNA ATCGTCTGCTCCGTCCAATATGATGACACCTGCATAATTTATAGTGAGTCTTGATTCACGCTGCATTTGGTGTGAGGTCGTGC 53.01%
Apta_743 DNA ATCGTCTGCTCCGTCCAATATCCGAGCAAGAACTCATATTGCATTATTTATAGCTACGCGCTGCATTTGGTGTGAGGTCGTGC 53.01%
Apta_791 DNA ATCGTCTGCTCCGTCCAATATATCAAAGGCGAATTTTGTCAAGGTGTTAAACGATAGTCCCTACCTTTGGTGTGAGGTCGTGC 53.01%
Apta_531 DNA ATACGGGAGCCAACACCACATCGACTTACGAATCAACGCGTTTATTATTGGTTCAGAGCAGGTGTGACGGAT 52.78%
Apta_920 DNA ATCCAGAGTGACGCAGCACGGCACTCACTCTTTGTTAAGTGGTCTGCTTCTTAACCTTCATCGACACGGTGGCTTA 52.63%
Apta_1088 DNA ATCCAGAGTGACGCAGCATCGAGTGGCTTGTTTGTAATGTAGGGTTCCGGTCGTGGGTTGGACACGGTGGCTTAGT 52.63%
Apta_104 DNA AGCAGCACAGAGGTCAGATGCAAACCACAGCCTGAGTGGTTAGCGTATGTCATTTACGGACCTATGCGTGCTACCGTGAA 52.50%
Apta_104 DNA AGCAGCACAGAGGTCAGATGCAAACCACAGCCTGAGTGGTTAGCGTATGTCATTTACGGACCTATGCGTGCTACCGTGAA 52.50%
Apta_226 DNA GGGAGATCACTTACGGCACCGTATAGTCGTACATGAACGAGTGTGAGTGTTAAGCCTTTGGCGACAGGGCTCGGAACC 52.50%
Apta_1118 DNA GGGAATGGATCCACATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGTTCACTGCAGACTTGACGAAGCTT 52.50%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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