Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 474
Aptamer sequence: CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAG
Target unique ID: Q61727
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_469 | R5A1 | Protein | I10R1_MOUSE | 12 nM | 150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature | 22434135 |
Structure information of aptamer
| Aptamer Sequence: | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAG |
| The optimal secondary structure in dot-bracket notation: | .(((..((((.((.((.....)).)).)))))))...... |
| The centroid secondary structure in dot-bracket notation: | ..(...((((.((.((.....)).)).)))).)....... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_469 | Description | R5A1 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 52.5% | Molecular weight | 12,218.88 Da |
| Molarity of 1 μg/μl solution | 81.84 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAG | ||
| Applications | Bound to IL-10 receptor on the cell surface and blocked IL-10 function in vitro | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | Q61727 | Protein name | Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210) |
| Gene name(s) | Il10ra Il10r | Organism | Interleukin-10 receptor subunit alpha (IL-10 receptor subunit alpha) (IL-10R subunit alpha) (IL-10RA) (CDw210a) (Interleukin-10 receptor subunit 1) (IL-10R subunit 1) (IL-10R1) (CD antigen CD210) |
| Status | reviewed in uniprot database | Length | 575 |
| Mass | 64,248 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. |
Activity data
 |
Interaction ID | 474 |
| Target type | Protein | |
| Target unique ID | Q61727 | |
| Activity | 12 nM | |
| Binding Conditions/Buffer | 150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82 °C and slowly cooling to room temperature |
|
| Assay | Inhibition of IL-10 dependent proliferation of MC/9 cells. MC/9 cells cultured in the presence of 1ng/ml IL-10 were incubated with increasing concentrations of either anti-IL-10 antibodies, tetrameric R5A1 aptamers or tetrameric R5B1 aptamers and proliferation was determined 48 hours later by measuring 3H-thymidine incorporation |
|
| PubMed ID | 22434135 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_468 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAT | |
| Apta_471 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACGCCCCAG | |
| Apta_470 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCTCAT | |
| Apta_3 | DNA | CCTGCCTTTGGCAGCCGGGCGAAGGCAACCCGACACCGACAGC | |
| Apta_597 | DNA | TACTGGGCTGGGCTTGGATTAGATCACAGTGGAACATACAGGACACACG | |
| Apta_4 | DNA | CCTGCCTTTGGAACCGACCGCCAGGCCGAGGAAAGGCACCGACAGC | |
| Apta_978 | DNA | GGCGATGAGAACGGGAGCCTGATGATACACACGAGCGATCGCTCATAAAAGCCGTGCCCG | |
| Apta_987 | DNA | GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC | |
| Apta_167 | DNA | TGCTCCTTGGCCTTAGCCCTGGAACCATCAATCCTCTTCG | |
| Apta_643 | DNA | ACCGATCCATCGAGTTTCTGAGAAAGGCCCGGAGAAACCGCGAGA | |
| Apta_316 | DNA | CCGCCCAAATCCCTAAGAGAAGACTGTAATGACATCAAACCAGACACACTACACACGCA | |
| Apta_144 | DNA | GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG | |
| Apta_162 | DNA | CCGTACATACTACGTACGTACGTGAACAGAGGCAGAG | |
| Apta_275 | DNA | AGTTGAATGGTGTGGTCACTTCCAGTCCCGCAGGGCACAC | |
| Apta_291 | DNA | AAAAATCTAGGCGCTGAAGCCCCGCTTCCTTACCTTCAC | |
| Apta_363 | DNA | ATTGACCGCTGTGTGACGCAACACTCAAT | |
| Apta_387 | DNA | CTTTAATGCCCTTCACTAGGTCTAAGCTACTACTTACCCG | |
| Apta_503 | DNA | ACTTTTGAATGTGGCAACAAATTCGACAGG | |
| Apta_789 | DNA | TAACTTTGAAGGAAAGGCTACAAACTCTTC | |
| Apta_973 | DNA | TATGTAATGTTGACGTAATTTATCTCTGTGAGAATAAGCAGGTGGTATCC |
DEV TOOL
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