AptaDB: Result

Interaction ID: 503

Aptamer sequence: GTTCGATTGGATTGTGCCGGAAGTGCTGGCTCGAAC

Target unique ID: Q988B7

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_497	pL1	Protein	PLDH_RHILO	16.8 nM	20 mM Tris-HCl pH 8.0, 50 mM NaCl, 5 mM KCl, and 5 mM MgCl2	22459583

Aptamer Sequence:	GTTCGATTGGATTGTGCCGGAAGTGCTGGCTCGAAC
The optimal secondary structure in dot-bracket notation:	((((((.........(((((.....)))))))))))
The centroid secondary structure in dot-bracket notation:	((((((.........(((((.....)))))))))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_497	Description	pL1
Aptamer chemistry	DNA	Length	36 nt
GC content	55.6%	Molecular weight	11,163.16 Da
Molarity of 1 μg/μl solution	89.58 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	GTTCGATTGGATTGTGCCGGAAGTGCTGGCTCGAAC
Applications	Tool for the diagnosis, monitoring, and surveillance of malaria

Type	Detail	Type	Detail
Uniprot ID	Q988B7	Protein name	Pyridoxal 4-dehydrogenase (tPLDH) (EC 1.1.1.107)
Gene name(s)	pldh-t mlr6807	Organism	Pyridoxal 4-dehydrogenase (tPLDH) (EC 1.1.1.107)
Status	reviewed in uniprot database	Length	248
Mass	25,531	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=NAD(+) + pyridoxal = 4-pyridoxolactone + H(+) + NADH
PDB ID(s)	3NDR;3NUG;3RWB;
Function	Involved in the degradation of pyridoxine or pyridoxamine.

	Interaction ID	503
	Target type	Protein
	Target unique ID	Q988B7
	Activity	16.8 nM
	Binding Conditions/Buffer	20 mM Tris-HCl pH 8.0, 50 mM NaCl, 5 mM KCl, and 5 mM MgCl2
	Assay	Dissociation constants of the pLDH aptamers determined by a fluorescence assay. The fluorescence intensities of FAM-modified aptamers were measured to determine the dissociation constants of the pL1 aptamer and the pL2 aptamer
	PubMed ID	22459583

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1087	DNA	CTTACGACTCAGGCATTTTGCCGGGTAACGAAGTTACTGTCGTAAG	58.70%
Apta_71	DNA	GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC	58.33%
Apta_498	DNA	GAACTCATTGGCTGGAGGCGGCAGTACCGCTTGAGTT	56.76%
Apta_47	DNA	GACCTAGCAGTGGACATGTGGCAGGGTGAAGTGGCATCGTC	56.10%
Apta_60	DNA	GGAATGAGTGCCCTGCAAGCGAGGGCTAGC	55.56%
Apta_125	DNA	TGGTGGATGGCGCAGTCGGCGACAA	55.56%
Apta_1024	DNA	GTCCAGGGTTCCAAGGTGCTTCGTGGAC	55.56%
Apta_911	DNA	GGCCATTGTGGACTTCTTTGGGTAATTCAGGGGCTCGATT	55.00%
Apta_912	DNA	TAGTATGGACTTGTCTTCTTGTCAGAGTCTGGCAGCACGT	55.00%
Apta_161	DNA	GTGTGCAGATCTGATTGCCTGCGTCAACCTACATACAAC	53.85%
Apta_1156	DNA	TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT	53.33%
Apta_486	DNA	GGGAGGGATTGGGTGGGCACAGCTCGCTGATAGGTCCTGGTCTCAC	53.19%
Apta_18	DNA	TGGGGGGTTGGACGGGCGGGGCTGC	52.78%
Apta_62	DNA	AGTCCGTGGTAGGGCAGGTTGGGGTGACT	52.78%
Apta_69	DNA	AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT	52.78%
Apta_70	DNA	AGGTCGTAGGTTGGTGTGGTTGGGGCGACCT	52.78%
Apta_72	DNA	AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT	52.78%
Apta_73	DNA	GGGGAGTAGGTTGGTGTGGTTGGGGCTCCCC	52.78%
Apta_74	DNA	GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC	52.78%
Apta_363	DNA	ATTGACCGCTGTGTGACGCAACACTCAAT	52.78%

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