Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 470
Aptamer sequence: AGCTGACACAGCAGGTTGGTGGGGGTGGCTTCCAGTTGGGTTGACAATACGTAGGGACACGAAGTCCAACCACGAGTCGAGCAATCTCGAAAT
Target unique ID: Q53HY0
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_465 | aptamer 40 | Protein | CONB1_LUPAL | 360 nM | 10 mM Phosphate, 138 mM NaCl, 2.7 mM KCl, 1.5 mM MgCl2, pH 7.4 | 22529997 |
Structure information of aptamer
| Aptamer Sequence: | AGCTGACACAGCAGGTTGGTGGGGGTGGCTTCCAGTTGGGTTGACAATACGTAGGGACACGAAGTCCAACCACGAGTCGAGCAATCTCGAAAT |
| The optimal secondary structure in dot-bracket notation: | .((((...)))).((((((.....(((..((((.((((......)))).....))))))).....)))))).....(((((....)))))... |
| The centroid secondary structure in dot-bracket notation: | .((((...)))).((((((.....(((........(((......)))..........))).....)))))).....(((((....)))))... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_465 | Description | aptamer 40 |
| Aptamer chemistry | DNA | Length | 93 nt |
| GC content | 53.8% | Molecular weight | 28,903.50 Da |
| Molarity of 1 μg/μl solution | 34.60 μM | Number of G-quadruplexes | 2 |
| G-Score | 21 | Function | Aptasensor |
| Sequence | AGCTGACACAGCAGGTTGGTGGGGGTGGCTTCCAGTTGGGTTGACAATACGTAGGGACACGAAGTCCAACCACGAGTCGAGCAATCTCGAAAT | ||
| Applications | Analysis of the Lup an 1 allergen in foodstuffs | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | Q53HY0 | Protein name | Conglutin beta 1 (Protein Lup-1) (allergen Lup an 1) [Cleaved into: Blad] |
| Gene name(s) | N/A | Organism | Conglutin beta 1 (Protein Lup-1) (allergen Lup an 1) [Cleaved into: Blad] |
| Status | reviewed in uniprot database | Length | 531 |
| Mass | 62,032 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | Seed storage protein. Accumulates during seed development and is hydrolyzed after germination to provide a carbon and nitrogen source for the developing seedling. |
Activity data
 |
Interaction ID | 470 |
| Target type | Protein | |
| Target unique ID | Q53HY0 | |
| Activity | 360 nM | |
| Binding Conditions/Buffer | 10 mM Phosphate, 138 mM NaCl, 2.7 mM KCl, 1.5 mM MgCl2, pH 7.4 |
|
| Assay | ELONA assay. Detection of ?-conglutin by ELONA using Sequence 40 in SELEX Binding Buffer at 100 nM final concentration |
|
| PubMed ID | 22529997 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_466 | DNA | AGCTGACACAGCAGGTTGGTGGGGGTGGCTCACATCATGGTAGAATGACTGAACAGCGTTGATTAAAAGGCACGAGTCGAGCAATCTCGAAAT | |
| Apta_100 | DNA | AGCAGCACAGAGGTCAGATGATTCAAGGGTAACACATCCAGGGGAACGGAATTGCAGCCCCCTATGCGTGCTACCGTGAA | |
| Apta_100 | DNA | AGCAGCACAGAGGTCAGATGATTCAAGGGTAACACATCCAGGGGAACGGAATTGCAGCCCCCTATGCGTGCTACCGTGAA | |
| Apta_176 | DNA | AGCAGCACAGAGGTCAGATGTATGGTACATTCGAGCGAGCTAAACTAGGATTAAACTCACCCTATGCGTGCTACCGTGAA | |
| Apta_228 | DNA | AGCAGCACAGAGGTCAGATGCGGCGCGGGGTCTTTTACAAATAGTTGTCCTAGCCACGCCTATGCGTGCTACCGTGAA | |
| Apta_236 | DNA | AGCAGCACAGAGGTCAGATGAGATGGTTGACCGGTTGATGTGATTAAGGAGACGTCGATTCCTATGCGTGCTACCGTGAA | |
| Apta_107 | DNA | AGCAGCACAGAGGTCAGATGTTCATTTCAGTTGACCGATAGCATTGAGCAATGATCTATTCCTATGCGTGCTACCGTGAA | |
| Apta_109 | DNA | AGCAGCACAGAGGTCAGATGTGCGACCACCTGGATAGACGAGGGTAGTAAGGAGTGTGTTCCTATGCGTGCTACCGTGA | |
| Apta_110 | DNA | AGCAGCACAGAGGTCAGATGGTTAGGAAGGTGAATAGGTACGACTGCAGGTCTATCGCAGCCTATGCGTGCTACCGTGAA | |
| Apta_107 | DNA | AGCAGCACAGAGGTCAGATGTTCATTTCAGTTGACCGATAGCATTGAGCAATGATCTATTCCTATGCGTGCTACCGTGAA | |
| Apta_109 | DNA | AGCAGCACAGAGGTCAGATGTGCGACCACCTGGATAGACGAGGGTAGTAAGGAGTGTGTTCCTATGCGTGCTACCGTGA | |
| Apta_110 | DNA | AGCAGCACAGAGGTCAGATGGTTAGGAAGGTGAATAGGTACGACTGCAGGTCTATCGCAGCCTATGCGTGCTACCGTGAA | |
| Apta_235 | DNA | AGCAGCACAGAGGTCAGATGTGGTTCGATGCTGCAAGGTGTTGTCATTTGGCATATGTTACCTATGCGTGCTACCGTGAA | |
| Apta_759 | DNA | GAGGCATACCAGCTTATTATTGGGGCCGGGGCAAGGGGGGGGTACCGTGGTAGGACAGATAGTAAGTGCAATCTGCGAA | |
| Apta_803 | DNA | ATGAGAAGCGTCGGTGTGGTTACTCCGGGCACTTGATATATCGATATGGAGAATAATGCACCCTGAGCGGGCTGGCAAGGCGCATA | |
| Apta_105 | DNA | AGCAGCACAGAGGTCAGATGATGCTCTAGGCGAAAGACTTGGCGTAGAACGACGGGAGGCCCTATGCGTGCTACCGTGAA | |
| Apta_105 | DNA | AGCAGCACAGAGGTCAGATGATGCTCTAGGCGAAAGACTTGGCGTAGAACGACGGGAGGCCCTATGCGTGCTACCGTGAA | |
| Apta_173 | DNA | AGCAGCACAGAGGTCAGATGGCAGGTTTCGACGTACAATGCTATGGAGGCTTTATGATCGCCTATGCGTGCTACCGTGAA | |
| Apta_175 | DNA | AGCAGCACAGAGGTCAGATGAGGACTGTCGATAAGTATAATGGCAAGTAGCTCGATACATCCTATGCGTGCTACCGTGAA | |
| Apta_278 | DNA | AGCAGCACAGAGGTCAGATGAGGCCTGGTGATCGTGCCCAGGCCATATGAGCAAGGAACCCCTATGCGTGCTACCGTGAA |
DEV TOOL
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