AptaDB: Result

Interaction ID: 412

Aptamer sequence: AAAGCCCACTGCATAGAGGCCGCTCAACATGCGAGCCGGT

Target unique ID: P49767

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_406	hVap2_T2160	Protein	VEGFC_HUMAN	107 nM	APBB; 10 mM HEPES, pH 7.9, 125 mM NaCl, 25 mM KCl, and 1 mM MgCl2	24675636

Aptamer Sequence:	AAAGCCCACTGCATAGAGGCCGCTCAACATGCGAGCCGGT
The optimal secondary structure in dot-bracket notation:	.................((((((.......))).)))...
The centroid secondary structure in dot-bracket notation:	.................((((((.......))).)))...

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_406	Description	hVap2_T2160
Aptamer chemistry	DNA	Length	40 nt
GC content	60.0%	Molecular weight	12,284.91 Da
Molarity of 1 μg/μl solution	81.40 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	AAAGCCCACTGCATAGAGGCCGCTCAACATGCGAGCCGGT
Applications	Demonstrated a potent inhibition of capillary tube formation of endothelial cells, comparable to the effect of a clinically approved anti-VEGF antibody drug, bevacizumab

Type	Detail	Type	Detail
Uniprot ID	P49767	Protein name	Vascular endothelial growth factor C (VEGF-C) (Flt4 ligand) (Flt4-L) (Vascular endothelial growth factor-related protein) (VRP)
Gene name(s)	VEGFC	Organism	Vascular endothelial growth factor C (VEGF-C) (Flt4 ligand) (Flt4-L) (Vascular endothelial growth factor-related protein) (VRP)
Status	reviewed in uniprot database	Length	419
Mass	46,883	Activity regulation	N/A
Involvement in disease	Lymphatic malformation 4 (LMPHM4)	Catalytic activity	N/A
PDB ID(s)	2X1W;2X1X;4BSK;6TJT;
Function	Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels.

	Interaction ID	412
	Target type	Protein
	Target unique ID	P49767
	Activity	107 nM
	Binding Conditions/Buffer	APBB; 10 mM HEPES, pH 7.9, 125 mM NaCl, 25 mM KCl, and 1 mM MgCl2
	Assay	Flow cytometry-based measurement of binding affinity of full-length and truncated aptamers to VEGF
	PubMed ID	24675636

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_158	DNA	CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTG	57.50%
Apta_339	DNA	CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTGT	57.50%
Apta_405	DNA	TCTAGCCCACTGCATAGAGGTAGCCACGTATGTGGGTGTTACCCATTCGTAGCTACCGGTG	55.74%
Apta_735	DNA	AGGCCCCCAGGCTCGGTGGATGCAAACACATGACTATGGGCCCGT	55.56%
Apta_498	DNA	GAACTCATTGGCTGGAGGCGGCAGTACCGCTTGAGTT	55.00%
Apta_937	DNA	TTGCCCACAGATCTGTGGAAGCCGAACCGCGTGCTAGTCGTG	54.76%
Apta_939	DNA	TTGCCCACCGTACTGTGCAGGTCGAACTACAGGCACGTCGTG	54.76%
Apta_1045	DNA	GGTGCTCAGGGAACTGTCTGAGGGATCAGGCTTAAGCCTGTCGAGCAGTT	54.00%
Apta_1218	DNA	CGACACCACACAGCAGTAAGCACTACAACATAGTGGTCTAGACTATCTCGGGCCGGTG	53.45%
Apta_641	DNA	TTCAAGACGATGCCTGGCGCGAGTTACACACTTGCATGGAGCTGG	53.33%
Apta_821	DNA	CACAGTCACTCAGACGGCCGCTATTGTTGCCAGATTGCCTTTGGC	53.33%
Apta_56	DNA	GCAGCTAAGCAGGCGGCTCACAAAACCATTCGCATGCGGC	52.50%
Apta_142	DNA	CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA	52.50%
Apta_166	DNA	ATCGCGCTCTCCTGCTTAAGCAGCTATCAAATAGCCCACT	52.50%
Apta_227	DNA	ACGCGCCTATGGTGCATCGCATGCCGACC	52.50%
Apta_273	DNA	ATAAGCTGAGCATCTTAGATCCCCGTCAAGGGCAGCGTAA	52.50%
Apta_302	DNA	ATCCTGTGAGGAATGCTCATGCATAGCAAGGGCT	52.50%
Apta_320	DNA	GCACTCTGTGGGGGTGGACGGGCCGGGT	52.50%
Apta_503	DNA	ACTTTTGAATGTGGCAACAAATTCGACAGG	52.50%
Apta_504	DNA	TTGCTCCTTATAGAGCCGTCCGAGC	52.50%

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