Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 320
Aptamer sequence: GTGGGGTTGGGTGGGTTTGGGTTATGGGCGCTCCGGCCCGGCGTCTTGCGTCCCTTCCAT
Target unique ID: P14210
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_311 | H38-21 | Protein | HGF_HUMAN | 20 nM | PBS containing 1 mM MgCl2 | 16225393 |
Structure information of aptamer
| Aptamer Sequence: | GTGGGGTTGGGTGGGTTTGGGTTATGGGCGCTCCGGCCCGGCGTCTTGCGTCCCTTCCAT |
| The optimal secondary structure in dot-bracket notation: | ....((..(((...((..(((...(((((......)))))...))).))..)))..)).. |
| The centroid secondary structure in dot-bracket notation: | ........(((.............(((((......)))))...........)))...... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_311 | Description | H38-21 |
| Aptamer chemistry | DNA | Length | 60 nt |
| GC content | 66.7% | Molecular weight | 18,847.74 Da |
| Molarity of 1 μg/μl solution | 53.06 μM | Number of G-quadruplexes | 3 |
| G-Score | 62 | Function | Targeted therapy |
| Sequence | GTGGGGTTGGGTGGGTTTGGGTTATGGGCGCTCCGGCCCGGCGTCTTGCGTCCCTTCCAT | ||
| Applications | Inhibited the biological activities of HGF including the stimulation of scattering, migration, and invasion of pancreatic cancer KP-3 cells | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P14210 | Protein name | Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain] |
| Gene name(s) | HGF HPTA | Organism | Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain] |
| Status | reviewed in uniprot database | Length | 728 |
| Mass | 83,134 | Activity regulation | N/A |
| Involvement in disease | Deafness, autosomal recessive, 39 (DFNB39) | Catalytic activity | N/A |
| PDB ID(s) | 1BHT;1GMN;1GMO;1GP9;1NK1;1SHY;1SI5;2HGF;2QJ2;3HMS;3HMT;3HN4;3MKP;3SP8;4D3C;4K3J;4O3T;4O3U;5COE;5CP9;5CS1;5CS3;5CS5;5CS9;5CSQ;5CT1;5CT2;5CT3;7MO7;7MO8;7MO9;7MOA;7MOB;7OCL;7OCM; |
 |
Function | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. |
Activity data
 |
Interaction ID | 320 |
| Target type | Protein | |
| Target unique ID | P14210 | |
| Activity | 20 nM | |
| Binding Conditions/Buffer | PBS containing 1 mM MgCl2 |
|
| Assay | Dose-dependent inhibition of HGF-induced migration of cells on the addition of H38-15 or H38-21. The concentration of HGF was 100 pM. The relative ratios of migrating cells for DNA/HGF (assigned as 100%) versus for each DNA are indicated. The means of the results obtained for 10 randomly selected microscopic fields in each well are shown with SD. The data shown are representative of three experiments |
|
| PubMed ID | 16225393 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_558 | DNA | CCGGTGGGTGGTCAGGTGGGATAGCGTTCCGCGTATGGCCCAGCGCATCACGGGTTCGCACCA | |
| Apta_341 | DNA | TCGGGGTTTGGGGGTTCGGGGTCGGTTCGGTTTCTTCTAATGGA | |
| Apta_565 | DNA | GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG | |
| Apta_609 | DNA | GCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCT | |
| Apta_610 | DNA | GCTGTGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCT | |
| Apta_509 | DNA | TCGCGCGAGTCGTCTGGGGGACTAGAGGACTTGTGCGGCCCCGCATCGTCCTCCC | |
| Apta_608 | DNA | TGAGGCGGGTGGGTGGGTTGAATACGCTGATCACCCCATCGGAGAACGTTAAGGCGCTTT | |
| Apta_611 | DNA | GCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTTGCTTAGCTTCTACGGTGGGCTATCT | |
| Apta_750 | DNA | ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT | |
| Apta_940 | DNA | TGTGCGGGGGAGGGGAGTGGCGCTGTTGGTGCGGGTATAGCGCGTGGTGT | |
| Apta_942 | DNA | AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA | |
| Apta_1014 | DNA | TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT | |
| Apta_798 | DNA | GTTCGTGGTGTGCTGGATGTCGGAGATGGTCCGCTTCGTTCAACTACTACGCATTTTGACACATCCAGCAGCACGA | |
| Apta_732 | DNA | AAGGAGCAGCGTGGAGGATAGCTGTCATCGGCTTATGTGCTTTCCAGGGTCCGTGCTGTCCGTTGTTAGGGTGTGTCGTCGTGGT | |
| Apta_769 | DNA | GAATTCAGTCGGACAGCGAGTTGTTGTTAGGGTGTTGGGTTGGGGTTTTAGGGTCCCTGTCGTCGATGGACGAATATCGTCTCCC | |
| Apta_255 | DNA | TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT | |
| Apta_356 | DNA | CGGGGGGTACGTGCCCGTGTTGCTCGGCGGCCCCCTCGCTTATTTTTGTGTGCCCTTT | |
| Apta_487 | DNA | CGGGGAGTTGGGAGGGGGGGCAAAGCCTGTGTATTTATCTTCTGT | |
| Apta_607 | DNA | TGAGGCGGGTGGGTGGGTTGAATATGCTGATTACCCCATCGGAGAACGTTAAGGCGCTTC | |
| Apta_749 | DNA | GATCGGTGGGTGGGGGGGTTGGAGATCATCCTCAGGGATTACGTC |
DEV TOOL
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