AptaDB: Result

Interaction ID: 302

Aptamer sequence: CGGCCACAGAAUGAAAAACCUCAUCGAUGUUGCAUAGUUG

Target unique ID: P10451

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_293	OPN-R3	Protein	OSTP_HUMAN	18 nM	PBS (137 nM NaCl, 27 nM KCl, 100 nM Na2HPO4, 2 nM K2HPO4, pH 7.4)	18985031

Aptamer Sequence:	CGGCCACAGAAUGAAAAACCUCAUCGAUGUUGCAUAGUUG
The optimal secondary structure in dot-bracket notation:	..((.(((..((((......))))...))).)).......
The centroid secondary structure in dot-bracket notation:	..((.(((..((((......))))...))).)).......

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_293	Description	OPN-R3
Aptamer chemistry	RNA	Length	40 nt
GC content	45.0%	Molecular weight	13,066.63 Da
Molarity of 1 μg/μl solution	76.53 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	CGGCCACAGAAUGAAAAACCUCAUCGAUGUUGCAUAGUUG
Applications	Decreases local progression and distant metastases

Type	Detail	Type	Detail
Uniprot ID	P10451	Protein name	Osteopontin (Bone sialoprotein 1) (Nephropontin) (Secreted phosphoprotein 1) (SPP-1) (Urinary stone protein) (Uropontin)
Gene name(s)	SPP1 BNSP OPN PSEC0156	Organism	Osteopontin (Bone sialoprotein 1) (Nephropontin) (Secreted phosphoprotein 1) (SPP-1) (Urinary stone protein) (Uropontin)
Status	reviewed in uniprot database	Length	314
Mass	35,423	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	3CXD;3DSF;
Function	Major non-collagenous bone protein that binds tightly to hydroxyapatite.

	Interaction ID	302
	Target type	Protein
	Target unique ID	P10451
	Activity	18 nM
	Binding Conditions/Buffer	PBS (137 nM NaCl, 27 nM KCl, 100 nM Na2HPO4, 2 nM K2HPO4, pH 7.4)
	Assay	OPN-R3 and adhesion, migration, and invasion of MDA-MB-231 cells
	PubMed ID	18985031

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_86	RNA	GAAACGACUCGACAACCUCGAGCGACGUGAAUCAU	55.00%
Apta_85	RNA	UGCGACUGUUAUAACCUAACAGCGACGUAAAGAUA	52.50%
Apta_87	RNA	UACCGACUAGCAAAACCUGCUGGCGACGUUUAGAU	52.50%
Apta_588	RNA	CCGGUGCGCAUAACCACCUCAGUGCGAGCAAGGAA	52.50%
Apta_374	RNA	GUCCCGUGCGCAAAAGUUCUUAGCGCUAGCAGUCCUAGUUGC	52.38%
Apta_242	RNA	GCUUCCAGGAAGAGAAGUGUCAUGUGAUCAGUCCUGGUGGGUGG	52.27%
Apta_1166	RNA	GAUGUAACUGAAUGAAAUGGUGAAGGACGGGUCCAGUAGGCUGC	52.27%
Apta_304	RNA	CGGCCGAUAGAAAGACUACUUGAGCCCUUAAAUGAGGUUAUGUGCGGCCG	52.00%
Apta_94	RNA	AUGACAGGAUGCAGAGCUCCACUGUCUAGUGUUUA	50.00%
Apta_95	RNA	CCGCGACAGUCGUAAGUUUUGACUGACUGAACGUU	50.00%
Apta_241	RNA	GCUUGACGGAGAUCAAGGGCGAGUCUCAUACCAAGUUGAUGGGG	50.00%
Apta_1292	RNA	GGCACGUGUAUUACCCUAGUGGUCGACGUGCC	50.00%
Apta_425	RNA	UGCCGCCAUAUCACACGGAUUUAAUCGCCGUAGAAAAGCAUGUCAAAGCCG	49.02%
Apta_426	RNA	UGCCGCUAUAAUGCACGGAUUUAAUCGCCGUAGAAAAGCAUGUCAAAGCCG	49.02%
Apta_1220	DNA	TGGCGACAAGAAGACGTACAAACACGCACCAGGCCGGAGTGGAGTATTCTGAGGTCGG	48.28%
Apta_431	RNA	CAGGCAUGCCUAGCUAAGCAGCCCAUGGCUUAUGCGCGGAAUAUUGGCUUCCGUUC	48.21%
Apta_1068	DNA	CGGGACAAGACAACGAACGAACAGGAAGAGAACCGGAATGCAGACGTCAGGG	48.08%
Apta_244	RNA	GCUUCCGGAGAGAAGGGUCAAAGUUGUGCGGGAGUGUGUUGUGG	47.73%
Apta_93	RNA	CUCCGCUAUCUAACGUAUGAUAGAAUGGAUGACUA	47.50%
Apta_230	RNA	GGGAGGACGAUGCGGGCGUGAAUGGUGUUGUGAGG	47.50%

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