AptaDB: Result

Interaction ID: 1045

Aptamer sequence: CCACACCGAGATGTTGTGACGGTTCCGATTGGCAAACTGA

Target unique ID: P01266

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_962	T-5	Protein	THYG_HUMAN	14.92 μM	TGK, Tris-HCl, BBS, and PBS	33303143

Aptamer Sequence:	CCACACCGAGATGTTGTGACGGTTCCGATTGGCAAACTGA
The optimal secondary structure in dot-bracket notation:	.((((.((...)).)))).(((((((....))..))))).
The centroid secondary structure in dot-bracket notation:	.((((.(.....).)))).(((((((....))..))))).

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_962	Description	T-5
Aptamer chemistry	DNA	Length	40 nt
GC content	52.5%	Molecular weight	12,320.93 Da
Molarity of 1 μg/μl solution	81.16 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	CCACACCGAGATGTTGTGACGGTTCCGATTGGCAAACTGA
Applications	CE-based detection validated the application feasibility of aptamer for Tg detection

Type	Detail	Type	Detail
Uniprot ID	P01266	Protein name	Thyroglobulin (Tg)
Gene name(s)	TG	Organism	Thyroglobulin (Tg)
Status	reviewed in uniprot database	Length	2768
Mass	304,790	Activity regulation	N/A
Involvement in disease	Thyroid dyshormonogenesis 3 (TDH3)	Catalytic activity	N/A
PDB ID(s)	6SCJ;7B75;
Function	Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).

No relevant experimental diagram	Interaction ID	1045
	Target type	Protein
	Target unique ID	P01266
	Activity	14.92 μM
	Binding Conditions/Buffer	TGK, Tris-HCl, BBS, and PBS
	Assay	N/A
	PubMed ID	33303143

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_648	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGC	55.00%
Apta_965	DNA	CACGGCATGTGTGGCCGGATGCCTATATCGGTCGACTGCA	55.00%
Apta_795	DNA	CGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGA	54.17%
Apta_573	DNA	CGTACAGTGGGCTATATTGCTGACGTAAGAGCTGCTTTCAATGCATTGGCATAGATGTCA	53.33%
Apta_752	DNA	GCTATCTTATGGAAATTTCGTGTAGGGTTTGGTGTGGCGGGGCTA	53.33%
Apta_845	DNA	CCAAACGCGCTGGTTTGGAATGCTTAGAGTTTGATCGATGCCTAA	53.33%
Apta_883	DNA	CGTACCGGCCAGTGATTACGACGAGACGAGCTTATGCGTATTGATGCCTAACTATCTACA	53.33%
Apta_65	DNA	CTACCGTGGTAGGGAAGGTTGGAGTGTAG	52.50%
Apta_75	DNA	CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG	52.50%
Apta_142	DNA	CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA	52.50%
Apta_423	DNA	TGGGAGCTGATGTCGCATGGGTTTTGATCACATGA	52.50%
Apta_436	DNA	CCCCCGCATCACGCCGTGGTGCGATTGACACAATTGCAAT	52.50%
Apta_446	DNA	AGCCATGACGATGTCGTTACGTAGATGCATAGACTCCTAA	52.50%
Apta_681	DNA	CCTGACGATCGGGTGTGGGTTGGCTTGAGG	52.50%
Apta_873	DNA	CCACCGAGCCTACCACATGTGACATCCCAGGACATAGCTG	52.50%
Apta_964	DNA	CACGAGAGGGGTGAGGTGTCGTATTAGAGGGAAGCTCCAA	52.50%
Apta_1065	DNA	CCTCGCTATGATGGAGTGCGTTTAGATCAGGGAACGGGTT	52.50%
Apta_1205	DNA	GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG	52.50%
Apta_1257	DNA	CGACGCACAGTCGCTGGTGCGTACCTGGTTGCCGTTGTGT	52.50%
Apta_1177	DNA	TCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGA	52.17%

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