Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 227
Aptamer sequence: CATTGAGATAGCTAGTTGTAGCTGCGTCATAGGCTGGGTTGGGTCTAGTGGTTGGGTGTG
Target unique ID: P05121
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_223 | PAI-1 01 | Protein | PAI1_HUMAN | 339 pM | PBSMCT (DPBS with 2.5 mM MgCl2, 1 mM CaCl2, 0.05% TWEEN-20) | 24644057 |
Structure information of aptamer
| Aptamer Sequence: | CATTGAGATAGCTAGTTGTAGCTGCGTCATAGGCTGGGTTGGGTCTAGTGGTTGGGTGTG |
| The optimal secondary structure in dot-bracket notation: | ...(((..((((((....))))))..)))((((((......))))))............. |
| The centroid secondary structure in dot-bracket notation: | .........(((((....)))))..................................... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_223 | Description | PAI-1 01 |
| Aptamer chemistry | DNA | Length | 60 nt |
| GC content | 51.7% | Molecular weight | 19,005.92 Da |
| Molarity of 1 μg/μl solution | 52.62 μM | Number of G-quadruplexes | 2 |
| G-Score | 19 | Function | Targeted binding |
| Sequence | CATTGAGATAGCTAGTTGTAGCTGCGTCATAGGCTGGGTTGGGTCTAGTGGTTGGGTGTG | ||
| Applications | Binds specifically to thrombin | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P05121 | Protein name | Plasminogen activator inhibitor 1 (PAI) (PAI-1) (Endothelial plasminogen activator inhibitor) (Serpin E1) |
| Gene name(s) | SERPINE1 PAI1 PLANH1 | Organism | Plasminogen activator inhibitor 1 (PAI) (PAI-1) (Endothelial plasminogen activator inhibitor) (Serpin E1) |
| Status | reviewed in uniprot database | Length | 402 |
| Mass | 45,060 | Activity regulation | N/A |
| Involvement in disease | Plasminogen activator inhibitor-1 deficiency (PAI-1D) | Catalytic activity | N/A |
| PDB ID(s) | 1A7C;1B3K;1C5G;1DB2;1DVM;1DVN;1LJ5;1OC0;3CVM;3EOX;3PB1;3Q02;3Q03;3R4L;3UT3;4AQH;4G8O;4G8R;4IC0;5BRR;5ZLZ;6GWN;6GWP;6GWQ;6I8S;6ZRV;7AQF;7AQG;9PAI; |
 |
Function | Serine protease inhibitor. Inhibits TMPRSS7. |
Activity data
 |
Interaction ID | 227 |
| Target type | Protein | |
| Target unique ID | P05121 | |
| Activity | 339 pM | |
| Binding Conditions/Buffer | PBSMCT (DPBS with 2.5 mM MgCl2, 1 mM CaCl2, 0.05% TWEEN-20) |
|
| Assay | Highest affinity aptamers obtained via particle display for four protein targets |
|
| PubMed ID | 24644057 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1230 | DNA | GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG | |
| Apta_79 | DNA | ATCTCGAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACTCGT | |
| Apta_217 | DNA | CGCCTGATTAGCGATACTCAGGCGTTGGGTGGGTGGGTGGG | |
| Apta_1185 | DNA | CAGGGGGTTTTGGTGGGGGGGGCTGGGTTGTCTTGGGGGTGGGCT | |
| Apta_801 | DNA | TGCGTGTGTAGTGTGTCTGCATGCCCCTGTAATCGCCCATGGGTAGCCTCTTAGGGATTTGGGCGG | |
| Apta_1229 | DNA | CACGGGCAGAGGGATAGGTTGTTGACGGGGCTGGTGGGTGGGTGCGCTCGCGCTATCGTGG | |
| Apta_656 | DNA | GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC | |
| Apta_344 | DNA | CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG | |
| Apta_651 | DNA | CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTG | |
| Apta_692 | DNA | ACGTTGACGCTGGTGCCCGCTTGTGGTGCGAGTGTTGTGT | |
| Apta_750 | DNA | ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT | |
| Apta_834 | DNA | CGCAATACCAAAGTGGCGAGAGCGCTGTCTTGAGTGAGTGGTTGG | |
| Apta_851 | DNA | CCATGTTTTGTAGGTGTAATAGGTCATGTTAGGGTTTCTG | |
| Apta_882 | DNA | AATAGTCTGGTTCGGTGCTTGGTGCGATGTGTTAGTCAGACTCTTGTGTG | |
| Apta_913 | DNA | GGCACTTGTTTGCTCGAACGATGGTTTGTGCTTTTGTGTT | |
| Apta_928 | DNA | GATTGAGTAGATAGTGGTTCTGTACGTAGTGAAAGAGTGG | |
| Apta_1080 | DNA | CTTACGACCCAGGGGGGTGGACAGGCGGGGGTTAGGGGGGTCGTAAG | |
| Apta_1186 | DNA | AGGGGGTTTTGGTGGGGGGGGCTGGGTTGTCTTGGGGGTGGGCT | |
| Apta_508 | DNA | GCGGGCGATCGGCGGGGGGTGCGTGCGCTCTGTGCCAGGGGGTGGGACAGATCATATGGGGGTGCT | |
| Apta_1117 | DNA | CGTACGGAATTCGCTAGCGGACTGGAGTCTAGACCGGGTAGCTGTGGTGGATCCGAGCTCCACGTG |
DEV TOOL
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