Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 498
Aptamer sequence: ATACGACTCACTATTAGGGACAAAGCTGACAACCCTTTCATAATCTAACTACATTTATGTGCTAGACTACTGACTAC
Target unique ID: P08263
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_944 | G1 | Protein | GSTA1_HUMAN | 59 nM | 20 mM Tris-HCl, 100 mM NaCl, 5 mM MgCl2, and 0.1% bovine serum albumin with 10 µg/mL yeast tRNA and 10µg/mL salmon sperm DNA | 33195056 |
Structure information of aptamer
| Aptamer Sequence: | ATACGACTCACTATTAGGGACAAAGCTGACAACCCTTTCATAATCTAACTACATTTATGTGCTAGACTACTGACTAC |
| The optimal secondary structure in dot-bracket notation: | ...............((((.............)))).(((((.((((..((((....)))).)))).)).))).... |
| The centroid secondary structure in dot-bracket notation: | ...............((((.............)))).(((((.((((..((((....)))).)))).)).))).... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_944 | Description | G1 |
| Aptamer chemistry | DNA | Length | 77 nt |
| GC content | 37.7% | Molecular weight | 23,560.18 Da |
| Molarity of 1 μg/μl solution | 42.44 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | ATACGACTCACTATTAGGGACAAAGCTGACAACCCTTTCATAATCTAACTACATTTATGTGCTAGACTACTGACTAC | ||
| Applications | G1 specifically bound to cognate target protein | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P08263 | Protein name | Glutathione S-transferase A1 (EC 2.5.1.18) (13-hydroperoxyoctadecadienoate peroxidase) (EC 1.11.1.-) (Androst-5-ene-3,17-dione isomerase) (EC 5.3.3.-) (GST HA subunit 1) (GST class-alpha member 1) (GST-epsilon) (GSTA1-1) (GTH1) [Cleaved into: Glutathione S-transferase A1, N-terminally processed] |
| Gene name(s) | GSTA1 | Organism | Glutathione S-transferase A1 (EC 2.5.1.18) (13-hydroperoxyoctadecadienoate peroxidase) (EC 1.11.1.-) (Androst-5-ene-3,17-dione isomerase) (EC 5.3.3.-) (GST HA subunit 1) (GST class-alpha member 1) (GST-epsilon) (GSTA1-1) (GTH1) [Cleaved into: Glutathione S-transferase A1, N-terminally processed] |
| Status | reviewed in uniprot database | Length | 222 |
| Mass | 25,631 | Activity regulation | The isomerase activity is inhibited by S-methylglutathione (GSMe) |
| Involvement in disease | N/A | Catalytic activity | Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+) |
| PDB ID(s) | 1GSD;1GSE;1GSF;1GUH;1K3L;1K3O;1K3Y;1LBK;1PKW;1PKZ;1PL1;1PL2;1USB;1XWG;1YDK;2R3X;2R6K;3I69;3I6A;3IK9;3KTL;3L0H;3Q74;3U6V;3ZFB;3ZFL;4HJ2;5JCU;5LCZ;5LD0;6ATO;6ATP;6ATQ;6ATR;6YAW;7BIB;7BIC; |
 |
Function | Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). |
Activity data
 |
Interaction ID | 498 |
| Target type | Protein | |
| Target unique ID | P08263 | |
| Activity | 59 nM | |
| Binding Conditions/Buffer | 20 mM Tris-HCl, 100 mM NaCl, 5 mM MgCl2, and 0.1% bovine serum albumin with 10 µg/mL yeast tRNA and 10µg/mL salmon sperm DNA |
|
| Assay | Isolated aptamers specifically recognize target proteins in Western blot. Purified proteins and E. coli cell lysates are separated on SDS-PAGE, stained with Coomassie Brilliant Blue (left) and probed with Cy5.5-labeled corresponding aptamers (right). The dissociation constants of aptamer G1 toward their cognate target proteins are determined to be in the nanomolar range by dot blot |
|
| PubMed ID | 33195056 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_931 | DNA | ATACGACTCACTATTAGGGACCACATAAACATGCATCGCTCAATAACGGGATATTGTTGTGCTAGACTACTGACTACAA | |
| Apta_932 | DNA | ATACGACTCACTATTAGGGATGTCCACCATATAGATCGATTTAAGTCCCTCGTTATTAATGCTAGACTACTGACTACAA | |
| Apta_993 | DNA | AATCCGACCACGAGAGCAGACCGACCACAGCATTATCTAAACCCATTAGTCTAGTCCTGG | |
| Apta_999 | DNA | ATAGGAGTCGACCGACCAGAGGACCCCGCTATCTAATTTATAGGCAATAAGTGCGTCTACATCTAGACTC | |
| Apta_1004 | DNA | ATAGGAGTCGACCGACCAGACCCGACCTCTCTTTCACTGTGACACAATGGTGCGTCTACATCTAGACTC | |
| Apta_1005 | DNA | ATAGGAGTCGACCGACCAGACCCGACCTCTCCTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC | |
| Apta_1007 | DNA | ATAGGAGTCGACCGACCAGACCCGACCTCTCTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC | |
| Apta_997 | DNA | ATAGGAGTCGACCGACCAGACCCCGCCTCTCCTTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC | |
| Apta_1003 | DNA | ATAGGAGTCGACCGACCAGACCCCGCCTCTCCTTTCATGTGACACAATGGTGCGTCTACATCTAGACTC | |
| Apta_811 | DNA | ATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT | |
| Apta_726 | DNA | TACCAGTGCGATGCTCAGGCCGATGTCAACTTTTTCTAACTCACTGGTTTTGCCTGACGCATTCGGTTGAC | |
| Apta_996 | DNA | ATAGGAGTCGACCGACCAGACAACCCTACGCGACCAACCAGATGACCTACGTGCGTCTACATCTAGACTC | |
| Apta_1111 | DNA | GTGCGTCGCATGATCGCGATTAGTGCGACCATGTACGATTCTCACGACAACGACACTGTACTTGAGGACTGTCTCC | |
| Apta_550 | DNA | GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATTTTACAGAACAACCAACGTCGCTCCGGGTACTTCTTCATCGAGATAGTAAGTGCAATCT | |
| Apta_627 | DNA | ATACCAGCTTATTCAATTATGCGGGGATGAGGCTTGATCTGTGGCTCTGATCCATGATCAGTCGTCTTACGTGCGGTCCAGATAGTAAGTGCAATCT | |
| Apta_627 | DNA | ATACCAGCTTATTCAATTATGCGGGGATGAGGCTTGATCTGTGGCTCTGATCCATGATCAGTCGTCTTACGTGCGGTCCAGATAGTAAGTGCAATCT | |
| Apta_792 | DNA | ATACCAGCTTATTCAATTCCCAAATTGCCACCACTTACAGCATGATAACATACTACATCTTTTCATCAAGATAGTAAGTGCAATCT | |
| Apta_740 | DNA | ATCGTCTGCTCCGTCCAATAAACCTGCATAATTTATAAGTCTAGACTGCTGCATTTGGTGTGAGGTCGTGC | |
| Apta_883 | DNA | CGTACCGGCCAGTGATTACGACGAGACGAGCTTATGCGTATTGATGCCTAACTATCTACA | |
| Apta_884 | DNA | ATACCAGCTTATTCAATTTGCACCACTTATTGTTACTAATCTGAGATAGTAAGTGCAATCT |
DEV TOOL
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