Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 504
Aptamer sequence: GAACTCATTGGCTGGAGGCGGCAGTACCGCTTGAGTT
Target unique ID: Q988B7
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_498 | pL2 | Protein | PLDH_RHILO | 31.7 nM | 20 mM Tris-HCl pH 8.0, 50 mM NaCl, 5 mM KCl, and 5 mM MgCl2 | 22459583 |
Structure information of aptamer
| Aptamer Sequence: | GAACTCATTGGCTGGAGGCGGCAGTACCGCTTGAGTT |
| The optimal secondary structure in dot-bracket notation: | .((((((..(((.((..((....)).))))))))))) |
| The centroid secondary structure in dot-bracket notation: | ..(((((...........(((.....)))..))))). |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_498 | Description | pL2 |
| Aptamer chemistry | DNA | Length | 37 nt |
| GC content | 56.8% | Molecular weight | 11,461.35 Da |
| Molarity of 1 μg/μl solution | 87.25 μM | Number of G-quadruplexes | 2 |
| G-Score | 20 | Function | Aptasensor |
| Sequence | GAACTCATTGGCTGGAGGCGGCAGTACCGCTTGAGTT | ||
| Applications | Tool for the diagnosis, monitoring, and surveillance of malaria | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | Q988B7 | Protein name | Pyridoxal 4-dehydrogenase (tPLDH) (EC 1.1.1.107) |
| Gene name(s) | pldh-t mlr6807 | Organism | Pyridoxal 4-dehydrogenase (tPLDH) (EC 1.1.1.107) |
| Status | reviewed in uniprot database | Length | 248 |
| Mass | 25,531 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | Reaction=NAD(+) + pyridoxal = 4-pyridoxolactone + H(+) + NADH |
| PDB ID(s) | 3NDR;3NUG;3RWB; |
 |
Function | Involved in the degradation of pyridoxine or pyridoxamine. |
Activity data
 |
Interaction ID | 504 |
| Target type | Protein | |
| Target unique ID | Q988B7 | |
| Activity | 31.7 nM | |
| Binding Conditions/Buffer | 20 mM Tris-HCl pH 8.0, 50 mM NaCl, 5 mM KCl, and 5 mM MgCl2 |
|
| Assay | Dissociation constants of the pLDH aptamers determined by a fluorescence assay. The fluorescence intensities of FAM-modified aptamers were measured to determine the dissociation constants of the pL1 aptamer and the pL2 aptamer |
|
| PubMed ID | 22459583 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_290 | DNA | GAACTCACCGGGAAGAAGCCCGTTCCGTCACAGACATGTT | |
| Apta_959 | DNA | GGACCGCTAGGTTCGTGGCTGAGATCACGCGATATCAGTA | |
| Apta_320 | DNA | GCACTCTGTGGGGGTGGACGGGCCGGGT | |
| Apta_497 | DNA | GTTCGATTGGATTGTGCCGGAAGTGCTGGCTCGAAC | |
| Apta_674 | DNA | GCACGATGGGGAAAGGGTCCCCCTGGGTTG | |
| Apta_411 | DNA | AGACGTAAGTTAATTGGACTTGGTCGTGTGCGGCACAGCGATTGAAAT | |
| Apta_32 | DNA | CGGTAAACTGCGTCCGAAGGGGCTGCAGTGACCCGAATGGGTCCG | |
| Apta_406 | DNA | AAAGCCCACTGCATAGAGGCCGCTCAACATGCGAGCCGGT | |
| Apta_579 | DNA | ACTGAGGGCACGGACAGGAGGGGGAGAGATGGCGTGAGGT | |
| Apta_913 | DNA | GGCACTTGTTTGCTCGAACGATGGTTTGTGCTTTTGTGTT | |
| Apta_1257 | DNA | CGACGCACAGTCGCTGGTGCGTACCTGGTTGCCGTTGTGT | |
| Apta_63 | DNA | GACCCGTGGTAGGGTAGGATGGGGTGGTC | |
| Apta_701 | DNA | GTACTGGCGTTCGGGGGGTACCGCTGC | |
| Apta_1149 | DNA | ATCATGGTGGGTATCGGCACTCGTTGGTTGAT | |
| Apta_1240 | DNA | GGAGTAGTGGGGATTGTGGTGTATGTTGTC | |
| Apta_882 | DNA | AATAGTCTGGTTCGGTGCTTGGTGCGATGTGTTAGTCAGACTCTTGTGTG | |
| Apta_1045 | DNA | GGTGCTCAGGGAACTGTCTGAGGGATCAGGCTTAAGCCTGTCGAGCAGTT | |
| Apta_47 | DNA | GACCTAGCAGTGGACATGTGGCAGGGTGAAGTGGCATCGTC | |
| Apta_968 | DNA | TAACACGACAGACGTTCGGAGGTCGAACCCTGACAGCGTGGGC | |
| Apta_255 | DNA | TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT |
DEV TOOL
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