AptaDB: Result

Interaction ID: 166

Aptamer sequence: CCGTACATACTACGTACGTACGTGAACAGAGGCAGAG

Target unique ID: P04183

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_162	TK1_apta39	Protein	KITH_HUMAN	1.7 nM	PBS	30900869

Aptamer Sequence:	CCGTACATACTACGTACGTACGTGAACAGAGGCAGAG
The optimal secondary structure in dot-bracket notation:	.(((((.(((...))).)))))...............
The centroid secondary structure in dot-bracket notation:	.(((((.(((...))).)))))...............

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_162	Description	TK1_apta39
Aptamer chemistry	DNA	Length	37 nt
GC content	51.4%	Molecular weight	11,656.31 Da
Molarity of 1 μg/μl solution	85.79 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	CCGTACATACTACGTACGTACGTGAACAGAGGCAGAG
Applications	Diagnostic assays

Type	Detail	Type	Detail
Uniprot ID	P04183	Protein name	Thymidine kinase, cytosolic (EC 2.7.1.21)
Gene name(s)	TK1	Organism	Thymidine kinase, cytosolic (EC 2.7.1.21)
Status	reviewed in uniprot database	Length	234
Mass	25,469	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=ATP + thymidine = ADP + dTMP + H(+)
PDB ID(s)	1W4R;1XBT;2ORV;2WVJ;
Function	Cell-cycle-regulated enzyme of importance in nucleotide metabolism.

No relevant experimental diagram	Interaction ID	166
	Target type	Protein
	Target unique ID	P04183
	Activity	1.7 nM
	Binding Conditions/Buffer	PBS
	Assay	N/A
	PubMed ID	30900869

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_163	DNA	GCGCATGACCATTGATGTCTGCACCATCAGAGGCAGAG	60.53%
Apta_1148	DNA	CCGTCTTCCAGACAAGAGTGCAGGG	56.76%
Apta_1068	DNA	CGGGACAAGACAACGAACGAACAGGAAGAGAACCGGAATGCAGACGTCAGGG	55.77%
Apta_436	DNA	CCCCCGCATCACGCCGTGGTGCGATTGACACAATTGCAAT	55.00%
Apta_438	DNA	AGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA	55.00%
Apta_438	DNA	AGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA	55.00%
Apta_443	DNA	GGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA	55.00%
Apta_444	DNA	AGCCATTACGATGTCGTTACGTAGATGCAGAGACTCCTAA	55.00%
Apta_445	DNA	AGCCATGACGATGTCGTTACGTAGATGTAGAGACTCCTAA	55.00%
Apta_446	DNA	AGCCATGACGATGTCGTTACGTAGATGCATAGACTCCTAA	55.00%
Apta_1018	DNA	ATCCCTGCAGTTTGGTAGGGTGCGAGCAACATCAGGCACCTGCAGG	54.35%
Apta_318	DNA	CCCGTCTTCCAGACAAGAGTGCAGGG	54.05%
Apta_347	DNA	AAAAAAAAAAAAGTACGTTCAGGAGCAGTGCGA	54.05%
Apta_158	DNA	CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTG	53.85%
Apta_32	DNA	CGGTAAACTGCGTCCGAAGGGGCTGCAGTGACCCGAATGGGTCCG	53.33%
Apta_640	DNA	CAATGGACCTATTCGAGTACTGAATAGAACAGTCGGCGCTCTGGG	53.33%
Apta_506	DNA	AAGCGCGCGAGACTACGTTTGTAGGGATACCGATGTCG	52.63%
Apta_149	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGATGGCGTGG	52.50%
Apta_274	DNA	GATACTGAGCATCGTACATGATCCCGCAACGGGCAGTATT	52.50%
Apta_339	DNA	CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTGT	52.50%

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