AptaDB: Result

Interaction ID: 245

Aptamer sequence: TTTTTAATTAAAGCTCGCCATCAAATAGCTTT

Target unique ID: P07288

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_240	PSap4#5	Protein	KLK3_HUMAN	<100 nM	10 mM Tris/HCl, 150 mM NaCl, 5 mM KCl, 5 mM MgCl2, 0.05% Tween 20, pH 7.4, r.t.	20692149

Aptamer Sequence:	TTTTTAATTAAAGCTCGCCATCAAATAGCTTT
The optimal secondary structure in dot-bracket notation:	.........((((((...........))))))
The centroid secondary structure in dot-bracket notation:	.........((((((...........))))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_240	Description	PSap4#5
Aptamer chemistry	DNA	Length	32 nt
GC content	28.1%	Molecular weight	9,987.26 Da
Molarity of 1 μg/μl solution	100.13 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	TTTTTAATTAAAGCTCGCCATCAAATAGCTTT
Applications	Sensing PSA concentrations between 40 and 100 nM

Type	Detail	Type	Detail
Uniprot ID	P07288	Protein name	Prostate-specific antigen (PSA) (EC 3.4.21.77) (Gamma-seminoprotein) (Seminin) (Kallikrein-3) (P-30 antigen) (Semenogelase)
Gene name(s)	KLK3 APS	Organism	Prostate-specific antigen (PSA) (EC 3.4.21.77) (Gamma-seminoprotein) (Seminin) (Kallikrein-3) (P-30 antigen) (Semenogelase)
Status	reviewed in uniprot database	Length	261
Mass	28,741	Activity regulation	Inhibited by SERPINA5
Involvement in disease	N/A	Catalytic activity	Reaction=Preferential cleavage: -Tyr-\|-Xaa-
PDB ID(s)	2ZCH;2ZCK;2ZCL;3QUM;
Function	Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

No relevant experimental diagram	Interaction ID	245
	Target type	Protein
	Target unique ID	P07288
	Activity	<100 nM
	Binding Conditions/Buffer	10 mM Tris/HCl, 150 mM NaCl, 5 mM KCl, 5 mM MgCl2, 0.05% Tween 20, pH 7.4, r.t.
	Assay	N/A
	PubMed ID	20692149

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1061	DNA	TATTTATTGAATGCTGTAATTTCGCCACGCGAAAGTAGG	53.85%
Apta_789	DNA	TAACTTTGAAGGAAAGGCTACAAACTCTTC	53.12%
Apta_1198	DNA	CTTTTAAGAACCGGACGAACTAGAATATTATATTTT	52.78%
Apta_34	DNA	TGTCACAATTTCCCCCCTCGCACATTTTGCAAGTAACTGA	52.50%
Apta_166	DNA	ATCGCGCTCTCCTGCTTAAGCAGCTATCAAATAGCCCACT	52.50%
Apta_472	DNA	CCCCCACATTCAAATGGCCCCCCTACTCAATTACTGCACA	52.50%
Apta_852	DNA	CATCTGTGTGAAGACTATATGGCGCTCACATATTTCTTTC	52.50%
Apta_1023	DNA	CTCCTTCAACGAAGTGGGTCTCCTTCAACGAAGTGGGTCTC	51.22%
Apta_1153	DNA	TATTTTTATATTTGTTTGACAGTCGCTTGCTTGTGTAGGCGTT	51.16%
Apta_167	DNA	TGCTCCTTGGCCTTAGCCCTGGAACCATCAATCCTCTTCG	50.00%
Apta_221	DNA	GTCCTAGCAGACACTCGGCCATCACGCCCATTGGTTTGCA	50.00%
Apta_470	DNA	CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCTCAT	50.00%
Apta_503	DNA	ACTTTTGAATGTGGCAACAAATTCGACAGG	50.00%
Apta_504	DNA	TTGCTCCTTATAGAGCCGTCCGAGC	50.00%
Apta_918	DNA	TTTACTTATTCAATTCCCGTGGGAAGGCTATAGAGGGGCCAGTCTATGAATAAGTTT	49.12%
Apta_489	DNA	GGGGTTAAATATTCCCACATTGCCTGCGCCAGTACAAATAG	48.78%
Apta_161	DNA	GTGTGCAGATCTGATTGCCTGCGTCAACCTACATACAAC	48.72%
Apta_306	DNA	CAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCG	48.72%
Apta_463	DNA	GTTCTCATGCACACTTATAGCGGGGGGATCCAGTACT	48.65%
Apta_970	DNA	TTATGTAGCATTTATGAAATTTTTAAATCAATTTACTATTGGTGGTATCC	48.00%

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