Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1023
Aptamer sequence: GACGCTTACTCAGGTGTGACTCGTCCGTCGGTTCGCCTTGCGAGTGCACGCCGCCAGCCGTTACATAAAGTCTGCCCACTCGAAGGACGCAGATGAAGTCTC
Target unique ID: Other-37
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_935 | GVI-11 | Other | GCRV-infected CIK cell | 278.66 nM | 10% FBS | 32959408 |
Structure information of aptamer
| Aptamer Sequence: | GACGCTTACTCAGGTGTGACTCGTCCGTCGGTTCGCCTTGCGAGTGCACGCCGCCAGCCGTTACATAAAGTCTGCCCACTCGAAGGACGCAGATGAAGTCTC |
| The optimal secondary structure in dot-bracket notation: | ((((((......((((((((((((.....((....))..)))))).))))))...)).)))).......((((((((.......))..))))))........ |
| The centroid secondary structure in dot-bracket notation: | (((.((......((((((((((((...............)))))).))))))...))..))).......((((((((.......))..))))))........ |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_935 | Description | GVI-11 |
| Aptamer chemistry | DNA | Length | 102 nt |
| GC content | 58.8% | Molecular weight | 31,366.01 Da |
| Molarity of 1 μg/μl solution | 31.88 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | GACGCTTACTCAGGTGTGACTCGTCCGTCGGTTCGCCTTGCGAGTGCACGCCGCCAGCCGTTACATAAAGTCTGCCCACTCGAAGGACGCAGATGAAGTCTC | ||
| Applications | Recognize and bind to GCRV-infected cells | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-37 | Name | GCRV-infected CIK cell |
Activity data
 |
Interaction ID | 1023 |
| Target type | Other | |
| Target unique ID | Other-37 | |
| Activity | 278.66 nM | |
| Binding Conditions/Buffer | 10% FBS |
|
| Assay | Apparent equilibrium dissociation constant (Kd) of each aptamer binding to target GCRV-CIK cells. The concentrations of FAM-labelled aptamer candidates were diluted to 0-1,000 nM in PBS, respectively, and then incubated with target GCRV-CIK cells (5 × 10**5) at 4°C for 30 min. The binding fluorescence intensities of aptamer-target cells were detected by flow cytometry. Kd of each aptamer candidate was calculated according to the equation Y = BmaxX/(Kd + X) by SigmaPlot software |
|
| PubMed ID | 32959408 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_933 | DNA | GACGCTTACTCAGGTGTGACTCGGGGTGTAGCTCGTTATGATTCGGACAAGACTTACCTTGCGCCTCTGGGATCGAAGGACGCAGATGAAGTCTC | |
| Apta_934 | DNA | GACGCTTACTCAGGTGTGACTCGTGAACCCACCTCAGGGCATCTTACATTTCTTCTAAGTTGTTACCATGTTTCGAAGGACGCAGATGAAGTCTC | |
| Apta_1182 | DNA | GAATTCAGTCGGACAGCGTCAGTCGTCCCTTTATGCTTGGTGCTCACCGTCTGAATTTCTTCAGATGGACGAATATCGTCTCCC | |
| Apta_1015 | DNA | ATACCAGCTTATTCAATTGACCCGTTTCGTTCCCTCTGGGAAGTTTAGCCCAGTTGCCTGGGCGATACCAAGATAGTAAGTGCAATCT | |
| Apta_1183 | DNA | GAATTCAGTCGGACAGCGGCGTGAGTCTTTTGCGGTGGCTCGGGTTCGAATATCTACGACCTTGATGGACGAATATCGTCTCCC | |
| Apta_866 | DNA | CTCCTCTGACTGTAACCACGACCGCCTTCCACCGTTCTCCACCACCCCTCAAACAACCCTGCATAGGTAGTCCAGAAGCC | |
| Apta_867 | DNA | CTCCTCTGACTGTAACCACGCCACCGAGCCTACCACATGTGACATCCCAGGACATAGCTGGCATAGGTAGTCCAGAAGCC | |
| Apta_1016 | DNA | ATACCAGCTTATTCAATTTCTTCGCCAGTGCCAGGATCTCAGTTGGCGGTTCATTAGCTGGGTTGGTCGAAGATAGTAAGTGCAATCT | |
| Apta_495 | DNA | GGGAATGGATCCACATCTACGTATTACTGCTGGTGACGAGGGCTAGACGACGTACCTTCACTGCAGACTTGACGAAGCTT | |
| Apta_555 | DNA | TGTACCGTCTGAGCGATTCGTACGGATTGGGTTTGCACTTTACCTGCGGTGCATCGCAGCCAGTCAGTGTTAAGGAGTGC | |
| Apta_1120 | DNA | GGGAATGGATCCACATCTACGAATTCGGAATGAGTGCCCTGCAAGCGAGGGCTAGCTTCACTGCAGACTTGACGAAGCTT | |
| Apta_1184 | DNA | GAATTCAGTCGGACAGCGTAATCCCTTGTGTTTGACGTTCGAAGATTATTTCTATCTTTTAGGATGGACGAATATCGTCTCCC | |
| Apta_173 | DNA | AGCAGCACAGAGGTCAGATGGCAGGTTTCGACGTACAATGCTATGGAGGCTTTATGATCGCCTATGCGTGCTACCGTGAA | |
| Apta_408 | DNA | GCAATGGTACGGTACTTCCGTCATCAGCTTGTGATGTGGATGCGAACTGCAAAAGTGCACGCTACTTTGCTAA | |
| Apta_461 | DNA | CGCTCGGCTTCACGAGATTCGTGTCGTTGTGTCCTGTACCCGCCTTGACCAGTCACTCTAGAGCATCCGGACTG | |
| Apta_587 | DNA | GGAATGGATCCACATCCATGGATGGGCAATGCGGGGTGGAGAATGGTTGCCGCACTTCGGCTTCACTGCAGACTTGACGAAGCTT | |
| Apta_626 | DNA | ATACCAGCTTATTCAATTGGTGGTAGGATGGGCTCGATCGAGGTCTTCAGTCGGCTCGGTTTAGTTTATGTTCTCCTGTAGATAGTAAGTGCAATCT | |
| Apta_704 | DNA | GTACGAATTCACGAGGTTGCCAGCGGGGCCAGCCACTTCTGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGCGAGCATGGAGTCGGATCCTCTA | |
| Apta_727 | DNA | ATACCAGCTTATTCAATTAGTCACACTTAGAGTTCTAACTGCTGCGCCGCCGGGAAAATACTGTACGGTTAGATAGTAAGTGCAATCT | |
| Apta_773 | DNA | GAATTCAGTCGGACAGCGGTGGTGCTTGTGTATGGGGGTGGTTGGTGGGTTTTATGCTGCTGGATGGACGAATATCGTCTCCC |
DEV TOOL
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