AptaDB: Result

Interaction ID: 489

Aptamer sequence: CAGGGAGGGACAGGGCGGGAATTTGTATGATGGTTCGTTTGCTCG

Target unique ID: Q7AP54

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_484	A3	Protein	HBP2_LISMO	80 nM	20 mM Tris-AcOH (pH 8.0), 100 mM NaCl, 200 mM KCl, 5 mM MgCl2, 0.5% Triton X-100, and 5% DMSO	22954361

Aptamer Sequence:	CAGGGAGGGACAGGGCGGGAATTTGTATGATGGTTCGTTTGCTCG
The optimal secondary structure in dot-bracket notation:	............(((((((((((........)))))..)))))).
The centroid secondary structure in dot-bracket notation:	.............................................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_484	Description	A3
Aptamer chemistry	DNA	Length	45 nt
GC content	55.6%	Molecular weight	14,084.01 Da
Molarity of 1 μg/μl solution	71.00 μM	Number of G-quadruplexes	3
G-Score	62	Function	Aptasensor
Sequence	CAGGGAGGGACAGGGCGGGAATTTGTATGATGGTTCGTTTGCTCG
Applications	Peroxidase activity and strong binding affinity to hemin

Type	Detail	Type	Detail
Uniprot ID	Q7AP54	Protein name	Hemin/hemoglobin-binding protein 2 (Hn/Hb-binding protein 2) (P64) (Surface virulence-associated protein A)
Gene name(s)	hbp2 svpA lmo2185	Organism	Hemin/hemoglobin-binding protein 2 (Hn/Hb-binding protein 2) (P64) (Surface virulence-associated protein A)
Status	reviewed in uniprot database	Length	569
Mass	63,380	Activity regulation	Is overexpressed in mecA, clpC and clpP mutants
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	4MYP;4NLA;
Function	Acts as an extracellular and cell wall-bound hemophore; scavenges host heme and hemoglobin from the environment and also serves as a cell wall receptor for both.

	Interaction ID	489
	Target type	Protein
	Target unique ID	Q7AP54
	Activity	80 nM
	Binding Conditions/Buffer	20 mM Tris-AcOH (pH 8.0), 100 mM NaCl, 200 mM KCl, 5 mM MgCl2, 0.5% Triton X-100, and 5% DMSO
	Assay	CD spectra of selected sequences (4 μM) in PB buffer without (black line) and with (red line) K+
	PubMed ID	22954361

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_335	DNA	ACCGGGCGTACACCGTCGCGGCACATGTCTGAATGCGTTTAGTCTCTGTG	58.00%
Apta_813	DNA	TAGAGATATGACAGCGGGGAAGGTTAAGAGGCGCTAGGAG	57.78%
Apta_486	DNA	GGGAGGGATTGGGTGGGCACAGCTCGCTGATAGGTCCTGGTCTCAC	57.45%
Apta_50	DNA	AGTGGCGAGGGGGGTAGGTAAAGTATCCTTAGTGTGTTG	55.56%
Apta_146	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCG	55.56%
Apta_263	DNA	AGAGGGGAGGGTCGGGTATCGGCGTGTTCGGGGGATCTGC	55.56%
Apta_343	DNA	AACAGAGGGACAAACGGGGGAAGATTTGACGTCGACGAC	55.56%
Apta_579	DNA	ACTGAGGGCACGGACAGGAGGGGGAGAGATGGCGTGAGGT	55.56%
Apta_861	DNA	CAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACGCAACCTG	55.56%
Apta_1210	DNA	GGGGAGGCAGTGTGTTGTGTCGTGTGTGTGCTTGG	55.56%
Apta_1240	DNA	GGAGTAGTGGGGATTGTGGTGTATGTTGTC	55.56%
Apta_1245	DNA	AGTGTGAAGTGGTGTGTGTTGTTGTTTGTC	55.56%
Apta_1262	DNA	ACAAGGGGTGCAGGGGTCCGCGATTGTGACTGTGTGTGCG	55.56%
Apta_1229	DNA	CACGGGCAGAGGGATAGGTTGTTGACGGGGCTGGTGGGTGGGTGCGCTCGCGCTATCGTGG	54.10%
Apta_124	DNA	GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC	53.33%
Apta_150	DNA	CCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGG	53.33%
Apta_375	DNA	TTGGGGAGGGGCACTGGGCAGTGTAATTTACGAAAGCGAG	53.33%
Apta_393	DNA	AGGAGGATAGTTCGGTGGCTGTTCAGGGTCTCCTCCT	53.33%
Apta_474	DNA	CAAAAAGATAGGTGGATTTTTCCCCGGATCGGTTTGTATG	53.33%
Apta_475	DNA	CAAAAAGATAGGTGGATTTTTCCCCGGGTCGGTTTGTATG	53.33%

ABOUT