Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1061
Aptamer sequence: GGCGATGAGAACGGGAGCCTGATGATACACACGAGCGATCGCTCATAAAAGCCGTGCCCG
Target unique ID: P04640
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_978 | OC2 | Protein | OSTCN_RAT | 55.5 nM | 2 mM MgCl2, 50 mM Tris (pH 7.5) and 150 mM NaCl in distilled water | 33379043 |
Structure information of aptamer
| Aptamer Sequence: | GGCGATGAGAACGGGAGCCTGATGATACACACGAGCGATCGCTCATAAAAGCCGTGCCCG |
| The optimal secondary structure in dot-bracket notation: | (((.(((((..(((..((.((.((.....)))).))..))))))))....)))....... |
| The centroid secondary structure in dot-bracket notation: | (((..((((..(((..((.((.((.....)))).))..))))))).....)))....... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_978 | Description | OC2 |
| Aptamer chemistry | DNA | Length | 60 nt |
| GC content | 58.3% | Molecular weight | 18,577.92 Da |
| Molarity of 1 μg/μl solution | 53.83 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Aptasensor |
| Sequence | GGCGATGAGAACGGGAGCCTGATGATACACACGAGCGATCGCTCATAAAAGCCGTGCCCG | ||
| Applications | Binding to target proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P04640 | Protein name | Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein) |
| Gene name(s) | Bglap Bglap2 | Organism | Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein) |
| Status | reviewed in uniprot database | Length | 99 |
| Mass | 10,927 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. |
Activity data
 |
Interaction ID | 1061 |
| Target type | Protein | |
| Target unique ID | P04640 | |
| Activity | 55.5 nM | |
| Binding Conditions/Buffer | 2 mM MgCl2, 50 mM Tris (pH 7.5) and 150 mM NaCl in distilled water |
|
| Assay | Determination of affinity/dissociation constant (Kd) of from the saturation binding curve |
|
| PubMed ID | 33379043 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_106 | DNA | AGCAGCACAGAGGTCAGATGGAGCCAGGAGTCGGCCTACAGAAGATTCTGATGTACCTCACCTATGCGTGCTACCGTGAA | |
| Apta_106 | DNA | AGCAGCACAGAGGTCAGATGGAGCCAGGAGTCGGCCTACAGAAGATTCTGATGTACCTCACCTATGCGTGCTACCGTGAA | |
| Apta_469 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAG | |
| Apta_471 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACGCCCCAG | |
| Apta_662 | DNA | GGCGCCAAACAGGACCACCATGACAATTACCCATACCACCTCATTATGCCCCATCTCCGC | |
| Apta_685 | DNA | GGGGCAACAAACACGGAAGAAAATGAATCTACATACGTGGACATATATCCTGCCGCGTG | |
| Apta_697 | DNA | CTCTCGGGACGACGGACGCTAATCTTACAAGGGCGTAGTGTATGTCGTCCC | |
| Apta_1107 | DNA | GCGCGATGGACTGTACCTTCGGCGTAACTGATATTCCTGATGATTCTGGCGAGACGTAGCACAAACCCGGTGTATA | |
| Apta_1108 | DNA | GTGCTTAGGTGCAGAACGCACACACGCTCGGTATGACGTGTTCGCTACGGTATAATTGCAGGCGCGAGCACAACAG | |
| Apta_1111 | DNA | GTGCGTCGCATGATCGCGATTAGTGCGACCATGTACGATTCTCACGACAACGACACTGTACTTGAGGACTGTCTCC | |
| Apta_175 | DNA | AGCAGCACAGAGGTCAGATGAGGACTGTCGATAAGTATAATGGCAAGTAGCTCGATACATCCTATGCGTGCTACCGTGAA | |
| Apta_618 | DNA | GCGGCAAATCAGCATACGAGTGTACAGGGACGGGACGGGTGGGTAAAAGGTGTCGCCTCAG | |
| Apta_1017 | DNA | TAGGGAATTCGTCGACGGATCCCTGCAGTTTGGTAGGGTGCGAGCAACATCAGGCACCTGCAGGTCGACGCATGCGCCG | |
| Apta_57 | DNA | GCGGGTCGGTTGCTCGCTTCGCCCGATCGGTCTAAGGGTG | |
| Apta_315 | RNA | GGGAGGACGAUGCGGUCGUAUUAUCCGCUGCACGCCAGACGACUCGCCCGA | |
| Apta_468 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAT | |
| Apta_470 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCTCAT | |
| Apta_556 | DNA | GGCTCTCGGGACGACATGGATTTTCCATCAACGAAGTGCGTCCGTCCC | |
| Apta_593 | DNA | CTCTCGGGACGACAGCCGAGTTGATTCAACAGCCGAGTCGTCCC | |
| Apta_597 | DNA | TACTGGGCTGGGCTTGGATTAGATCACAGTGGAACATACAGGACACACG |
DEV TOOL
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