Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 918
Aptamer sequence: CCGGAGGTGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGACCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA
Target unique ID: 25922
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_832 P12-55 Cell Escherichia Coli (E.Coli) 161 nM PBS containing 1.4 mM MgCl2 27104834
Structure information of aptamer
Aptamer Sequence: CCGGAGGTGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGACCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA
The optimal secondary structure in dot-bracket notation: (((....)))....(((((((.(((((((..........(((.......)))..))))))).)))))))...........
The centroid secondary structure in dot-bracket notation: ((......))....(((((((.(((((((..........(((.......)))..))))))).)))))))...........
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_832 Description P12-55
Aptamer chemistry DNA Length 80 nt
GC content 71.3% Molecular weight 25,291.04 Da
Molarity of 1 μg/μl solution 39.54 μM Number of G-quadruplexes 3
G-Score 49 Function Aptasensor
Sequence CCGGAGGTGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGACCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA
Applications Diagnosis of MNEC-borne pathologies
Target information
Type Detail Type Detail
ATCC ID 25922 Cell name Escherichia Coli (E.Coli)
Category Bacteria Organism N/A
Product type Preceptrol culture Cell type N/A
Morphology N/A Tissue N/A
Disease N/A Product format Freeze-dried
Antigen expression N/A Genes expressed N/A
Cell description This strain does not produce verotoxin. This organism is a CLSI control strain for antimicrobial susceptibility testing. Used in susceptibility disc testing of neomycin, colistin [colimycin], kanamycin, cephalexin, gentamicins, cefamandole, cephalothin, tetracycline, cephaloglycin, cephaloridine [cephalomycin], nalidixic acid, and chloramphenicol.
Application(s) Bioinformatics; Food testing; Media testing; Quality control
Activity data
Interaction ID 918
Target type Cell
Target unique ID 25922
Activity 161 nM
Binding Conditions/Buffer

PBS containing 1.4 mM MgCl2
Assay

Binding affinity of selected pool 12 aptamers to E coli ATCC 25922
PubMed ID 27104834
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_732 DNA AAGGAGCAGCGTGGAGGATAGCTGTCATCGGCTTATGTGCTTTCCAGGGTCCGTGCTGTCCGTTGTTAGGGTGTGTCGTCGTGGT 56.47%
Apta_508 DNA GCGGGCGATCGGCGGGGGGTGCGTGCGCTCTGTGCCAGGGGGTGGGACAGATCATATGGGGGTGCT 56.25%
Apta_151 DNA GCAATGGTACGGTACTTCCGCGGTCCGGGGTGGGTGGGTGGTGGGGGGTGCGGGGGGGCGGCCGCAAAAGTGCACGCTACTTTGCTAA 55.68%
Apta_589 DNA GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG 55.00%
Apta_617 DNA CAATGGGGTCGGGCGGGCCGGGTGTCATGGTGGACGGAGATGGGACGTAGAGGGCGGT 55.00%
Apta_942 DNA AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA 55.00%
Apta_1071 DNA TTGAAGTGACTCCGCACTGGGTGGGTGGGAGGGTCGTGCGGCTGGTCATAGCAGGGT 55.00%
Apta_589 DNA GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG 55.00%
Apta_1229 DNA CACGGGCAGAGGGATAGGTTGTTGACGGGGCTGGTGGGTGGGTGCGCTCGCGCTATCGTGG 55.00%
Apta_731 DNA AAGGAGCAGCGTGGAGGATACTCTAATTGCCGTATAAGGTCAGGGGGTTGGTTGGTTCCTAGTGCTTAGGGTGTGTCGTCGTGGT 54.12%
Apta_68 DNA AGATGCCTGTCGAGCATGCTGTGAATAGGTAGGGTCGGATGGGCTACGGTGTAGCTAAACTGCTTTGTCGACGGG 53.75%
Apta_1115 DNA CGTACGGAATTCGCTAGCGGTGACGGACGTGGGGTGCACGAAGGGAGGGGATCCGAGCTCCACGTG 53.75%
Apta_44 DNA TCTCGGACGCGTGTGGTCGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTTCTTTGTTTGATCTTTCTCGCTGCCTGGCCCTAGAGTG 52.87%
Apta_223 DNA CATTGAGATAGCTAGTTGTAGCTGCGTCATAGGCTGGGTTGGGTCTAGTGGTTGGGTGTG 52.50%
Apta_418 DNA TGGCAGGAAGACAAACACATCGTCGCGGCCTTAGGAGGGGCGGACGGGGGGGGGCGTGGTCTGTGGTGCTGT 52.50%
Apta_568 DNA CCCAGCAGCACTGGTAGTGAGGCAGTTCACCGGTGGGGCGGTGAGTTTGGCTGCTATTTAT 52.50%
Apta_801 DNA TGCGTGTGTAGTGTGTCTGCATGCCCCTGTAATCGCCCATGGGTAGCCTCTTAGGGATTTGGGCGG 52.50%
Apta_837 DNA TGAGCCCAAGCCCTGGTATGTGATCGGTGACGAGGGTGCGGGGCGGGGGGTGAGGCACAGGGCAGGTCTACTTTGGGATC 52.50%
Apta_1082 DNA GGCTGGTCCGCTGGGAACAAGGGCGGGAGGGAGGGTGTGGGTGCGACAAGCGGACCAGCC 52.50%
Apta_951 DNA CACCTAATACGACTCACTATAGCGGATCCGAGGGGGTAGCGGGTGGGCCGGTGGATGCGGGGCGCCGGCGCCTGGCTCGAACAAGCTTGC 52.22%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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