AptaDB: Result

Interaction ID: 1153

Aptamer sequence: AGGCCCTACACGCCTTTTAGCGAGGGCGTTCCGTGGAGGA

Target unique ID: PDM-225

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1097	S8	Cell	HCM-BROD-0020-C15	7.90 nM	4.5 g/L glucose, 5 mM MgCl2, 1 mg/mL bovine serum albumin (BSA) and 0.1 mg/mL yeast tRNA in 0.01 M PBS	34105524

Aptamer Sequence:	AGGCCCTACACGCCTTTTAGCGAGGGCGTTCCGTGGAGGA
The optimal secondary structure in dot-bracket notation:	...((((((((((((((....))))))))...)))).)).
The centroid secondary structure in dot-bracket notation:	...((((((((((((((....))))))))...)))).)).

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1097	Description	S8
Aptamer chemistry	DNA	Length	40 nt
GC content	62.5%	Molecular weight	12,353.91 Da
Molarity of 1 μg/μl solution	80.95 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	AGGCCCTACACGCCTTTTAGCGAGGGCGTTCCGTGGAGGA
Applications	Specific imaging of ESCC tissues

Type	Detail	Type	Detail
ATCC ID	PDM-225	Cell name	HCM-BROD-0020-C15
Category	Human cells	Organism	Homo sapiens, human
Product type	N/A	Cell type	N/A
Morphology	N/A	Tissue	Esophagus
Disease	Cancer; Primary	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	N/A
Application(s)	3D cell culture; Cancer research

	Interaction ID	1153
	Target type	Cell
	Target unique ID	PDM-225
	Activity	7.90 nM
	Binding Conditions/Buffer	4.5 g/L glucose, 5 mM MgCl2, 1 mg/mL bovine serum albumin (BSA) and 0.1 mg/mL yeast tRNA in 0.01 M PBS
	Assay	The determination of the dissociation constant (Kd) of the selected aptamers
	PubMed ID	34105524

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_463	DNA	GTTCTCATGCACACTTATAGCGGGGGGATCCAGTACT	55.00%
Apta_974	DNA	CATGGTGTCAATACTATACCTTTTGCCGATGGTAACAGTTGGTGGTATCC	54.00%
Apta_1062	DNA	GGGGTCTTGCAGGTCCCGTAGGAGGGGCCATTGGAGTGGGG	53.66%
Apta_910	DNA	TCACGTGCATGATAGACGGCGAAGCCGTCGAGTTGCTGTGTGCCGATGCACGTGA	52.73%
Apta_1172	DNA	GACGCCACTGACTACCCCAGTATCTCTTCAACGCGTATTGAAGTCAGTCGGTCGTCA	52.63%
Apta_186	DNA	AGGAACGCTTTAGGTCTGAGATCTCGGAAT	52.50%
Apta_190	DNA	AGGAACCCTTTAGGTCTGAGATCTCGGAAT	52.50%
Apta_194	DNA	AGGAAGGCTTTAGGGTCTGAGATCTCGGGAAT	52.50%
Apta_308	DNA	TGGCGGTACGGGCCGTGCACCCACTTACCTGGGAAGTGA	52.50%
Apta_506	DNA	AAGCGCGCGAGACTACGTTTGTAGGGATACCGATGTCG	52.50%
Apta_1019	DNA	ATCCCTGCAGTTTGGTAGGGTGCAGG	52.50%
Apta_1067	DNA	GCGCGGTACCGCGCTAACGGAGGTTGCGTCCGT	52.50%
Apta_1200	DNA	GGCACACAGGACTATACAGTGTTGCAGTGTTGCTG	52.50%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	52.50%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	52.50%
Apta_144	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG	52.27%
Apta_595	DNA	AGCGAGGGCGGTGTCCAACAGCGGTTTTTTCACGAGGAGGTTGGCGGTGG	52.00%
Apta_930	DNA	TTGCACTATGTTTTAGCTAGGGTTCCCTCCGGAGATAGTAAGTGCAA	51.06%
Apta_287	DNA	CAGCACCGACCTTGTGCTTTGGGAGTGCTGGTCCAAGGGCGTTAATGGACA	50.98%
Apta_65	DNA	CTACCGTGGTAGGGAAGGTTGGAGTGTAG	50.00%

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