Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 714
Aptamer sequence: GGACAACATAGGAAAAAGGCTCTGCTACCGGATCCCTGTTGTATGGGCATATCTGTTGAT
Target unique ID: 6437088
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_661 | HC1 | Molecule | Microcystin YR | 193 nM | 50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM MgCl2 | 22958101 |
Structure information of aptamer
| Aptamer Sequence: | GGACAACATAGGAAAAAGGCTCTGCTACCGGATCCCTGTTGTATGGGCATATCTGTTGAT |
| The optimal secondary structure in dot-bracket notation: | ......((..(((.....(((((((.((.((...)).)).))).))))...)))..)).. |
| The centroid secondary structure in dot-bracket notation: | ...........((.....(((((((.((.((...)).)).))).))))...))....... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_661 | Description | HC1 |
| Aptamer chemistry | DNA | Length | 60 nt |
| GC content | 46.7% | Molecular weight | 18,553.91 Da |
| Molarity of 1 μg/μl solution | 53.90 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Aptasensor |
| Sequence | GGACAACATAGGAAAAAGGCTCTGCTACCGGATCCCTGTTGTATGGGCATATCTGTTGAT | ||
| Applications | Application in microcystin detection | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| PubChem ID | 6437088 | Molecular name | Microcystin YR |
| Molecular formula | C52H72N10O13 | Molecular weight | 1045.2 |
| IUPAC name | (5R,8S,11R,12S,15S,18S,19S,22R)-15-[3-(diaminomethylideneamino)propyl]-8-[(4-hydroxyphenyl)methyl]-18-[(1E,3E,5S,6S)-6-methoxy-3,5-dimethyl-7-phenylhepta-1,3-dienyl]-1,5,12,19-tetramethyl-2-methylidene-3,6,9,13,16,20,25-heptaoxo-1,4,7,10,14,17,21-heptazacyclopentacosane-11,22-dicarboxylic acid | InChIKey | OWHASZQTEFAUJC-GJRPNUFSSA-N |
| LogP | 0.521 | Topological polar surface area | 363.37 |
| Hydrogen bond acceptor | 12 | Hydrogen bond donor | 11 |
Synonym(s): Microcystin YR; 101064-48-6; Microcystin-YR; UNII-4G08121T5U; CHEMBL4633668; 4G08121T5U; Cyanoginosin-YR; Cyanoginosin LA, 3-tyrosyl-5-arginine-; cyanoginosin LA, 3-L-tyrosyl-5-L-arginine; MCYR; Cyanoginosin LA, 3-L-tyrosine-5-L-arginine; DTXSID00880086; BDBM50542676; (5R,8S,11R,12S,15S,18S,19S,22R)-15-[3-(diaminomethylideneamino)propyl]-8-[(4-hydroxyphenyl)methyl]-18-[(1E,3E,5S,6S)-6-methoxy-3,5-dimethyl-7-phenylhepta-1,3-dienyl]-1,5,12,19-tetramethyl-2-methylidene-3,6,9,13,16,20,25-heptaoxo-1,4,7,10,14,17,21-heptazacyclopentacosane-11,22-dicarboxylic acid; C19997; Q27259546
Activity data
 |
Interaction ID | 704 |
| Target type | Molecule | |
| Target unique ID | 6437382 | |
| Activity | 808 nM | |
| Binding Conditions/Buffer | 50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM MgCl2 |
|
| Assay | Determination of the dissociation constants (KD) for aptamers. On the basis of the fluorescence recovered, saturation curves were obtained and the dissociation constants were calculated by nonlinear regression analysis |
|
| PubMed ID | 22958101 | |
 |
Interaction ID | 713 |
| Target type | Molecule | |
| Target unique ID | 445434 | |
| Activity | 705 nM | |
| Binding Conditions/Buffer | 50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM MgCl2 |
|
| Assay | Determination of the dissociation constants (KD) for aptamers. On the basis of the fluorescence recovered, saturation curves were obtained and the dissociation constants were calculated by nonlinear regression analysis |
|
| PubMed ID | 22958101 | |
 |
Interaction ID | 714 |
| Target type | Molecule | |
| Target unique ID | 6437088 | |
| Activity | 193 nM | |
| Binding Conditions/Buffer | 50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM MgCl2 |
|
| Assay | Determination of the dissociation constants (KD) for aptamers. On the basis of the fluorescence recovered, saturation curves were obtained and the dissociation constants were calculated by nonlinear regression analysis |
|
| PubMed ID | 22958101 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1267 | DNA | GGCACGGCAAAGGGGTACAGCCTACCGAACCGTGGCTGTAAGGGTGGTTGTGGTGTG | |
| Apta_527 | DNA | ATACGGGAGCCAACACCAACAACCTAGATGTTCTGATAACACGAATCGCTTCGAAGAGCAGGTGTGACGGAT | |
| Apta_919 | DNA | GGAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCG | |
| Apta_106 | DNA | AGCAGCACAGAGGTCAGATGGAGCCAGGAGTCGGCCTACAGAAGATTCTGATGTACCTCACCTATGCGTGCTACCGTGAA | |
| Apta_106 | DNA | AGCAGCACAGAGGTCAGATGGAGCCAGGAGTCGGCCTACAGAAGATTCTGATGTACCTCACCTATGCGTGCTACCGTGAA | |
| Apta_838 | DNA | TGAGCCCAAGCCCTGGTATGTAGTAATGGTGCGTACAGGCGACGGGGTCCAGGCTGGAGGGGCAGGTCTACTTTGGGATC | |
| Apta_903 | DNA | ATACGGGAGCCAACACCAGACGGTAAATAAAGTCAGTATCGATACTCTATTCGAGAGCAGGTGTGACGGAT | |
| Apta_987 | DNA | GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC | |
| Apta_1152 | DNA | GACAAGGATAAATCCTTCAATGAAGTGGGTCACTCATCTGTGA | |
| Apta_1271 | DNA | ACCACAGCCAGTAAGTCCCGTAGGCCCTGGTTGGGCTGTT | |
| Apta_732 | DNA | AAGGAGCAGCGTGGAGGATAGCTGTCATCGGCTTATGTGCTTTCCAGGGTCCGTGCTGTCCGTTGTTAGGGTGTGTCGTCGTGGT | |
| Apta_904 | DNA | ATACGGGAGCCAACACCAGACGGTAAATAAAGTCAGTATCGAGTACTCTATTCGAGAGCAGGTGTGACGGAT | |
| Apta_1105 | DNA | TACGACTCACTATAGGGATCCAATGGCACAGCGCCTGGAACGTACTCTGTACCTGGAATTCCCTTTAGTGAGGGTT | |
| Apta_233 | DNA | AGCAGCACAGAGGTCAGATGTGAAACATAGCATATTTACTTATGTCGCCTTGCCGGTTCCTATGCGTGCTACCGTGAA | |
| Apta_61 | DNA | AGCAGCACAGAGGTCAGATGGCAGGTAAGCAGGCGGCTCACAAAACCATTCGCATGCGGCCCTATGCGTGCTACCGTGAA | |
| Apta_104 | DNA | AGCAGCACAGAGGTCAGATGCAAACCACAGCCTGAGTGGTTAGCGTATGTCATTTACGGACCTATGCGTGCTACCGTGAA | |
| Apta_104 | DNA | AGCAGCACAGAGGTCAGATGCAAACCACAGCCTGAGTGGTTAGCGTATGTCATTTACGGACCTATGCGTGCTACCGTGAA | |
| Apta_174 | DNA | AGCAGCACAGAGGTCAGATGTCGGTCTTGTGTATACATACCCGTGTGTTTTCATCTCAGGCCTATGCGTGCTACCGTGAA | |
| Apta_404 | DNA | GTCTTGACTAGTTACGCCCACAAGAACCTGCTTGCCATGGTGACGCCGATCATGCTTTTTGGTCATTCAGTTGGCGCCTC | |
| Apta_569 | DNA | TGCGCATGTGAAAGGTTGCCTAACTGGACAGGGTTTAGGAGCGACTAGACACAG |
DEV TOOL
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