Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1152
Aptamer sequence: CTTCTTCGTTAGAAGCCTGTCAATGGCCAGGGGGGAGGGG
Target unique ID: PDM-225
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1096 | S3 | Cell | HCM-BROD-0020-C15 | 19.77 nM | 4.5 g/L glucose, 5 mM MgCl2, 1 mg/mL bovine serum albumin (BSA) and 0.1 mg/mL yeast tRNA in 0.01 M PBS | 34105524 |
Structure information of aptamer
| Aptamer Sequence: | CTTCTTCGTTAGAAGCCTGTCAATGGCCAGGGGGGAGGGG |
| The optimal secondary structure in dot-bracket notation: | (((((((.((....(((.......)))...)).))))))) |
| The centroid secondary structure in dot-bracket notation: | ...((((.......(((.......)))......))))... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1096 | Description | S3 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 60.0% | Molecular weight | 12,448.97 Da |
| Molarity of 1 μg/μl solution | 80.33 μM | Number of G-quadruplexes | 2 |
| G-Score | 20 | Function | Aptasensor |
| Sequence | CTTCTTCGTTAGAAGCCTGTCAATGGCCAGGGGGGAGGGG | ||
| Applications | Specific imaging of ESCC tissues | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | PDM-225 | Cell name | HCM-BROD-0020-C15 |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | N/A | Cell type | N/A |
| Morphology | N/A | Tissue | Esophagus |
| Disease | Cancer; Primary | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | N/A |
| Cell description | N/A |
|
|
| Application(s) | 3D cell culture; Cancer research | ||
Activity data
 |
Interaction ID | 1152 |
| Target type | Cell | |
| Target unique ID | PDM-225 | |
| Activity | 19.77 nM | |
| Binding Conditions/Buffer | 4.5 g/L glucose, 5 mM MgCl2, 1 mg/mL bovine serum albumin (BSA) and 0.1 mg/mL yeast tRNA in 0.01 M PBS |
|
| Assay | The determination of the dissociation constant (Kd) of the selected aptamers |
|
| PubMed ID | 34105524 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1061 | DNA | TATTTATTGAATGCTGTAATTTCGCCACGCGAAAGTAGG | |
| Apta_596 | DNA | GGTGGCTGTTGGTCGAGGCTTATTAGTGTAGTCAATGGAGGCAGGCGACG | |
| Apta_652 | DNA | CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGGCAGGGGGTGTGCGTG | |
| Apta_682 | DNA | CCTTGTAGATCGGGTGTGGTTTGGCGTAGG | |
| Apta_344 | DNA | CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG | |
| Apta_33 | DNA | GCGGTAAACTGCGTCCGAAGGGGCTGCAGTGACCCGAATGGGTCCGCCGCGAAGCG | |
| Apta_651 | DNA | CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTG | |
| Apta_600 | DNA | GTCTCTGTGTGCGCCAGAGAACACTGGGGCAGATATGGGCCAGCACAGAATGAGGCCC | |
| Apta_600 | DNA | GTCTCTGTGTGCGCCAGAGAACACTGGGGCAGATATGGGCCAGCACAGAATGAGGCCC | |
| Apta_217 | DNA | CGCCTGATTAGCGATACTCAGGCGTTGGGTGGGTGGGTGGG | |
| Apta_565 | DNA | GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG | |
| Apta_1062 | DNA | GGGGTCTTGCAGGTCCCGTAGGAGGGGCCATTGGAGTGGGG | |
| Apta_32 | DNA | CGGTAAACTGCGTCCGAAGGGGCTGCAGTGACCCGAATGGGTCCG | |
| Apta_449 | DNA | CGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCG | |
| Apta_642 | DNA | ATCCAATACCTCAGAACTCAGTTCGAGTCGTAAAGGGGAATCGCA | |
| Apta_752 | DNA | GCTATCTTATGGAAATTTCGTGTAGGGTTTGGTGTGGCGGGGCTA | |
| Apta_1156 | DNA | TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT | |
| Apta_592 | DNA | CTCTCGGGACGACCACTACGCATAGTTTCTATCGCCAGGAAGGGTCGTCCC | |
| Apta_75 | DNA | CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG | |
| Apta_142 | DNA | CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA |
DEV TOOL
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