Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 919
Aptamer sequence: ATTTCGCCCCCGTGTTCCGACTGGTATCTTCACGTCTTCGAGTGT
Target unique ID: 25922
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_833 | 20-5 | Cell | Escherichia Coli (E.Coli) | 3.9 nM | 1X PBS, 0.1 mg/mL salmon sperm, 1% bovine serum albumin, and 0.05% Tween-20 | 31837967 |
Structure information of aptamer
| Aptamer Sequence: | ATTTCGCCCCCGTGTTCCGACTGGTATCTTCACGTCTTCGAGTGT |
| The optimal secondary structure in dot-bracket notation: | ..((((....((((..((....))......))))....))))... |
| The centroid secondary structure in dot-bracket notation: | ...(((....((((..((....))......))))....))).... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_833 | Description | 20-5 |
| Aptamer chemistry | DNA | Length | 45 nt |
| GC content | 53.3% | Molecular weight | 13,711.77 Da |
| Molarity of 1 μg/μl solution | 72.93 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | ATTTCGCCCCCGTGTTCCGACTGGTATCTTCACGTCTTCGAGTGT | ||
| Applications | Isolation of bacteria | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | 25922 | Cell name | Escherichia Coli (E.Coli) |
| Category | Bacteria | Organism | N/A |
| Product type | Preceptrol culture | Cell type | N/A |
| Morphology | N/A | Tissue | N/A |
| Disease | N/A | Product format | Freeze-dried |
| Antigen expression | N/A | Genes expressed | N/A |
| Cell description | This strain does not produce verotoxin. This organism is a CLSI control strain for antimicrobial susceptibility testing. Used in susceptibility disc testing of neomycin, colistin [colimycin], kanamycin, cephalexin, gentamicins, cefamandole, cephalothin, tetracycline, cephaloglycin, cephaloridine [cephalomycin], nalidixic acid, and chloramphenicol. |
|
|
| Application(s) | Bioinformatics; Food testing; Media testing; Quality control | ||
Activity data
 |
Interaction ID | 919 |
| Target type | Cell | |
| Target unique ID | 25922 | |
| Activity | 3.9 nM | |
| Binding Conditions/Buffer | 1X PBS, 0.1 mg/mL salmon sperm, 1% bovine serum albumin, and 0.05% Tween-20 |
|
| Assay | Binding saturation curve of aptamers |
|
| PubMed ID | 31837967 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_963 | DNA | TGGCGCTGCCCCTAGTCTCCGGCTGATAGCTGCTTCTTGG | |
| Apta_182 | DNA | AAAAAAGCCCCCCCTTCGACCGTATACCAGGGTACGGAATG | |
| Apta_220 | DNA | ATCAACTCACCGTAGGTCTAGTGAGAACTTCAAGTCTACT | |
| Apta_692 | DNA | ACGTTGACGCTGGTGCCCGCTTGTGGTGCGAGTGTTGTGT | |
| Apta_939 | DNA | TTGCCCACCGTACTGTGCAGGTCGAACTACAGGCACGTCGTG | |
| Apta_1176 | DNA | AGTATGATATCGGTGTTTATGGTGTCTGTCTTCATACT | |
| Apta_1263 | DNA | CCCCGCTGCCCGTGTTCCGTCCTCCCTTGCTGTGTGTGCG | |
| Apta_882 | DNA | AATAGTCTGGTTCGGTGCTTGGTGCGATGTGTTAGTCAGACTCTTGTGTG | |
| Apta_1231 | DNA | ACCACCGGCCCGAGATAGTCTAGACCACTATGTTGTTGTGCTTACTGCTGTGTGGTGTGG | |
| Apta_7 | DNA | CCAGTCTCCCGTTTACCGCGCCTACACATGTCTGAATGCC | |
| Apta_159 | DNA | GCGTGCAGCATGACCACCGATGTCACACTTAATGAGAAT | |
| Apta_307 | DNA | CATTCACCATGGCCCCTTCCTACGTATGTTCTGCGGGTG | |
| Apta_437 | DNA | TCACAGTCCCGGTGCCGCACTAAAACCCATTGTTGTGCGA | |
| Apta_518 | DNA | GTATTGCGGAGGAAGGTTTTTAACCTTCGGGGA | |
| Apta_519 | DNA | GTATTGCGGAGGAAGGTTTTTAACCTTCGGGG | |
| Apta_691 | DNA | CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT | |
| Apta_730 | DNA | ACCAAACACAGATGCAACCTGACTTCTAACGTCATTTGGTG | |
| Apta_853 | DNA | TGACCGAGTGTCTGGTCATTTTCGATGTCTGTTGTTAGGC | |
| Apta_877 | DNA | GTGCGAAGCGCCTCCACTGTACATCCACTCCTTCTGCC | |
| Apta_914 | DNA | CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT |
DEV TOOL
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