Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1105
Aptamer sequence: CTTCTGCCCGCCTCCTTCCCGGTCGAGACCCGGGTTTACTTCCTCGGTAGTGTTCGAGGGAGACGAGATAGGCGGACACT
Target unique ID: P02144
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1029 | M02 | Protein | MYG_HUMAN | 46.3 nM | Ammonium bicarbonate buffer (50 mM, pH 8.5) containing 500 mM NaCl | 32631049 |
Structure information of aptamer
| Aptamer Sequence: | CTTCTGCCCGCCTCCTTCCCGGTCGAGACCCGGGTTTACTTCCTCGGTAGTGTTCGAGGGAGACGAGATAGGCGGACACT |
| The optimal secondary structure in dot-bracket notation: | ....((.((((((.((((((((.......)))))....(((((((((......)))))))))..)))..)))))).)).. |
| The centroid secondary structure in dot-bracket notation: | .....(.((((((.((.(((((.......)))))....(((((((((......)))))))))...))..)))))).)... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1029 | Description | M02 |
| Aptamer chemistry | DNA | Length | 80 nt |
| GC content | 61.3% | Molecular weight | 24,606.68 Da |
| Molarity of 1 μg/μl solution | 40.64 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target recognition |
| Sequence | CTTCTGCCCGCCTCCTTCCCGGTCGAGACCCGGGTTTACTTCCTCGGTAGTGTTCGAGGGAGACGAGATAGGCGGACACT | ||
| Applications | Detecting Mb in human serum and distinguishing acute myocardial infarction patients from healthy individuals | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P02144 | Protein name | Myoglobin |
| Gene name(s) | MB | Organism | Myoglobin |
| Status | reviewed in uniprot database | Length | 154 |
| Mass | 17,184 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | 3RGK; |
 |
Function | Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. |
Activity data
 |
Interaction ID | 1105 |
| Target type | Protein | |
| Target unique ID | P02144 | |
| Activity | 46.3 nM | |
| Binding Conditions/Buffer | Ammonium bicarbonate buffer (50 mM, pH 8.5) containing 500 mM NaCl |
|
| Assay | Characterization of selected Mb-binding aptamers |
|
| PubMed ID | 32631049 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1030 | DNA | CTTCTGCCCGCCTCCTTCCCGGGTTACAGGATCGTAAATCTTATTCGTTGCTCGATCGGGAGACGAGATAGGCGGACACT | |
| Apta_1028 | DNA | CTTCTGCCCGCCTCCTTCCGGCGTCCCTGCGCACTGTATAACGTACACACGATACGTGGGAGACGAGATAGGCGGACACT | |
| Apta_1074 | DNA | CTTCTGCCCGCCTCCTTCCGCCACAGTATTATGTATCACAGTGCTTCATCTAATATGAGGAGACGAGATAGGCGGACACT | |
| Apta_1073 | DNA | CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT | |
| Apta_1075 | DNA | CTTCTGCCCGCCTCCTTCCTACTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT | |
| Apta_1076 | DNA | CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACACCCCCATAGGAGACGAGATAGGCGGACACT | |
| Apta_530 | DNA | ATCCGTCACACCTGCTCTGTGTACGCCCGTATTTACGTTCCTAGCAATTGCTATGTGGTGTTGGCTCCCGTAT | |
| Apta_617 | DNA | CAATGGGGTCGGGCGGGCCGGGTGTCATGGTGGACGGAGATGGGACGTAGAGGGCGGT | |
| Apta_906 | DNA | ATCCGTCACACCTGCTCTCCCCGGCGTCCGTTTATTAGCAGACTTTGGCGGAATTGGTGTTGGCTCCCGTAT | |
| Apta_1050 | DNA | ATCCGTCACACCTGCTCTCACGTCCGTCAACCAGATTGATACTTTATCTTGTTGTGGTGTTGGCTCCCGTAT | |
| Apta_1099 | DNA | ATCCATTGCCACTGACTACCAGCCGGGTGGGTGGGGGGGTTTGCTGGTATCGCATTGTGCGAAGTCAGTCGGTCGTTAGT | |
| Apta_1227 | DNA | CACACCAAACACACAAGTGGACCCTGACGCATGGATAGGGTGACGGTATACGCGGGCATG | |
| Apta_953 | DNA | TCACCTCATACGACTCACTATAGCGGATCCGAGCCGGGGTGTTCTGTTGGCGGGGGCGGTGGGCGGGCTGGCTCGAACAAGCTTGC | |
| Apta_31 | DNA | ATACCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGGTGTGCTGGCCAGATAGTATGTGCAATCA | |
| Apta_281 | DNA | CCGTAATACGACTCACTATAGGGGAGCTCGGTACCGAATTCATGTTGGTAGTTGGCTTGTGTTCGTGTGTTAAGCTTTCAGAGAGGATCCTT | |
| Apta_569 | DNA | TGCGCATGTGAAAGGTTGCCTAACTGGACAGGGTTTAGGAGCGACTAGACACAG | |
| Apta_596 | DNA | GGTGGCTGTTGGTCGAGGCTTATTAGTGTAGTCAATGGAGGCAGGCGACG | |
| Apta_657 | DNA | CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC | |
| Apta_734 | DNA | TCCTCGATGCACCAGTTCACCTTATTTGCTTCTTCTCTCTGTTCTGACTGGGTGCTGAGGA | |
| Apta_826 | DNA | ATCCGTCACACCTGCTCTGCACGGGCTCAGTTTGGCTTTGTATCCTAAGAGGGATGGTGTTGGCTCCCGTAT |
DEV TOOL
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