AptaDB: Result

Interaction ID: 838

Aptamer sequence: GGGGGACACGGAGGTGGTGGAAAGGCTAAGATTTGATGATGAGTAGTGTGGT

Target unique ID: CCL-2

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_756	aptamer 28	Cell	Hela cells	6.9 nM	1% BSA in PBS with salts	22536456

Aptamer Sequence:	GGGGGACACGGAGGTGGTGGAAAGGCTAAGATTTGATGATGAGTAGTGTGGT
The optimal secondary structure in dot-bracket notation:	.....((((....((.((.(((.........))).)).)).....))))...
The centroid secondary structure in dot-bracket notation:	.....((((....((.((.(((.........))).)).)).....))))...

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_756	Description	aptamer 28
Aptamer chemistry	DNA	Length	52 nt
GC content	51.9%	Molecular weight	16,428.53 Da
Molarity of 1 μg/μl solution	60.87 μM	Number of G-quadruplexes	2
G-Score	21	Function	Aptasensor
Sequence	GGGGGACACGGAGGTGGTGGAAAGGCTAAGATTTGATGATGAGTAGTGTGGT
Applications	Molecular probes of HPV-associated cervical cancer cells

Type	Detail	Type	Detail
ATCC ID	CCL-2	Cell name	Hela cells
Category	Human cells	Organism	Homo sapiens, human
Product type	N/A	Cell type	epithelial cell
Morphology	epithelial	Tissue	Uterus; Cervix
Disease	Adenocarcinoma	Product format	Frozen
Antigen expression	N/A	Genes expressed	Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway; keratin positive by immunoperoxidase staining
Cell description	HeLa cells are the first immortal human cells to be grown in culture and the basis for countless significant scientific discoveries. They were isolated in 1951 from a cervical carcinoma derived from a 31-year-old patient.
Application(s)	3D cell culture

	Interaction ID	838
	Target type	Cell
	Target unique ID	CCL-2
	Activity	6.9 nM
	Binding Conditions/Buffer	1% BSA in PBS with salts
	Assay	Binding equilibrium for aptamers and their HF cell epitopes
	PubMed ID	22536456

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_50	DNA	AGTGGCGAGGGGGGTAGGTAAAGTATCCTTAGTGTGTTG	59.62%
Apta_834	DNA	CGCAATACCAAAGTGGCGAGAGCGCTGTCTTGAGTGAGTGGTTGG	59.62%
Apta_1261	DNA	GGGCGGACGCTAGGTGGTGATGCTGTGCTACACGTGTTGT	59.62%
Apta_487	DNA	CGGGGAGTTGGGAGGGGGGGCAAAGCCTGTGTATTTATCTTCTGT	57.69%
Apta_691	DNA	CGACGCGCGTTGGTGGTGGATGGTGTGTTACACGTGTTGT	55.77%
Apta_753	DNA	GGGCACAGACGGAAGATGAGAATTGTGGGGCTTAGTATAGTGAGGTGCGTGT	55.77%
Apta_795	DNA	CGCAGCAAGGAGATTCGAGGGGGAAGGTTTGTTATAGGGGTTAATGGA	55.77%
Apta_1186	DNA	AGGGGGTTTTGGTGGGGGGGGCTGGGTTGTCTTGGGGGTGGGCT	55.77%
Apta_1189	DNA	GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG	55.77%
Apta_1205	DNA	GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG	55.77%
Apta_140	DNA	GGCAGGAAGACAAACACGATGGGGGGGTATGATTTGATGTGGTTGTTGCATGATCGTGGTCTGTGGTGCTGT	55.56%
Apta_417	DNA	GGCAGGAAGACAAACACCAGGTGAGGGAGACGACGCGAGTGTTAGATGGTAGCTGTTGGTCTGTGGTGCTGT	55.56%
Apta_901	DNA	ATACGGGAGCCAACACCACAAGTTGACTTGGAGGAGGGTCTAACTAGTTCGAGGAGAGCAGGTGTGACGGAT	55.56%
Apta_686	DNA	GGTCACCAACAACAGGGAGCGCTACGCGAAGGGTCAATGTGACGTCATGCGGATGTGTGG	55.00%
Apta_1230	DNA	GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG	55.00%
Apta_508	DNA	GCGGGCGATCGGCGGGGGGTGCGTGCGCTCTGTGCCAGGGGGTGGGACAGATCATATGGGGGTGCT	54.55%
Apta_564	DNA	GGGGACTGTTGTGGTATTATTTTTGGTTGTGCAGT	53.85%
Apta_579	DNA	ACTGAGGGCACGGACAGGAGGGGGAGAGATGGCGTGAGGT	53.85%
Apta_745	DNA	GGTTGCATGCCGTGGGGAGGGGGGTGGGTTTTATAGCGTACTCAG	53.85%
Apta_750	DNA	ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT	53.85%

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