AptaDB: Result

Interaction ID: 317

Aptamer sequence: TGGCGGTACGGGCCGTGCACCCACTTACCTGGGAAGTGA

Target unique ID: P14151

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_308	LD196	Protein	LYAM1_HUMAN	3.1 nM	20 mM Hepes (pH 7.5), 125 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 5 mM KCl plus 0.01% (wt/vol) human serum albumin	8981912

Aptamer Sequence:	TGGCGGTACGGGCCGTGCACCCACTTACCTGGGAAGTGA
The optimal secondary structure in dot-bracket notation:	..(((((....)))))...((((......))))......
The centroid secondary structure in dot-bracket notation:	..(((((....)))))...((((......))))......

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_308	Description	LD196
Aptamer chemistry	DNA	Length	39 nt
GC content	64.1%	Molecular weight	12,289.66 Da
Molarity of 1 μg/μl solution	81.37 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	TGGCGGTACGGGCCGTGCACCCACTTACCTGGGAAGTGA
Applications	Inhibit L-selectin binding to immobilized SLex in static assays and inhibit L-selectin-mediated rolling of human lymphocytes and neutrophils on cytokine-activated endothelial cells in flow-based assays

Type	Detail	Type	Detail
Uniprot ID	P14151	Protein name	L-selectin (CD62 antigen-like family member L) (Leukocyte adhesion molecule 1) (LAM-1) (Leukocyte surface antigen Leu-8) (Leukocyte-endothelial cell adhesion molecule 1) (LECAM1) (Lymph node homing receptor) (TQ1) (gp90-MEL) (CD antigen CD62L)
Gene name(s)	SELL LNHR LYAM1	Organism	L-selectin (CD62 antigen-like family member L) (Leukocyte adhesion molecule 1) (LAM-1) (Leukocyte surface antigen Leu-8) (Leukocyte-endothelial cell adhesion molecule 1) (LECAM1) (Lymph node homing receptor) (TQ1) (gp90-MEL) (CD antigen CD62L)
Status	reviewed in uniprot database	Length	372
Mass	42,187	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	2LGF;3CFW;5VC1;
Function	Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells.

No relevant experimental diagram	Interaction ID	317
	Target type	Protein
	Target unique ID	P14151
	Activity	3.1 nM
	Binding Conditions/Buffer	20 mM Hepes (pH 7.5), 125 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 5 mM KCl plus 0.01% (wt/vol) human serum albumin
	Assay	N/A
	PubMed ID	8981912

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_641	DNA	TTCAAGACGATGCCTGGCGCGAGTTACACACTTGCATGGAGCTGG	55.56%
Apta_165	DNA	TGGACGAACTGCCCTCAGCTACTTTCATGTTGCTGACGCA	55.00%
Apta_144	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG	54.55%
Apta_145	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGA	53.85%
Apta_420	DNA	AGGGACGGGCTAAGTAACTGTGGAGGGT	53.85%
Apta_143	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAGTTTCG	53.06%
Apta_263	DNA	AGAGGGGAGGGTCGGGTATCGGCGTGTTCGGGGGATCTGC	52.50%
Apta_264	DNA	GGCGTTGTCGGGCGCAGGTGTAGGCCTCGTGGTGGTGGGT	52.50%
Apta_375	DNA	TTGGGGAGGGGCACTGGGCAGTGTAATTTACGAAAGCGAG	52.50%
Apta_817	DNA	TGCGTGAGCGGTAGGCCCCTACGACCCACTGTGGTTGGGC	52.50%
Apta_1097	DNA	AGGCCCTACACGCCTTTTAGCGAGGGCGTTCCGTGGAGGA	52.50%
Apta_1259	DNA	GGCGCGGCATGGTGTGGACTCCAGGCGGTAGGGATGTCGT	52.50%
Apta_971	DNA	TAGTCTAGGAACGGTACGATCGTATTCCAAGGACTTTGCTGGTGGTATCC	52.00%
Apta_146	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCG	51.28%
Apta_197	DNA	GGCTTTAGGTCTGAGATCTCGGAAT	51.28%
Apta_325	DNA	TACTGCATGCACACCACTTCAACTA	51.28%
Apta_325	DNA	TACTGCATGCACACCACTTCAACTA	51.28%
Apta_390	DNA	GGTGCGGCGGGACTAGTGGGTGTG	51.28%
Apta_462	DNA	GGTATAGACCCCTGAGTCCTACCGAGGGCACGGCA	51.28%
Apta_829	DNA	CCCTCCGGGGGGGTCATCGGGATACCTGGTAAGGATA	51.28%

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