AptaDB: Result

Interaction ID: 390

Aptamer sequence: AUGAUCAAUCGCCUCAAUUCGACAGGAGGCUCAC

Target unique ID: P30530

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_384	GL21.T	Protein	UFO_HUMAN	12 nM	Dulbecco's Modified Eagle's Medium (DMEM), 10% FBS, 2mM L-glutamine	22910292

Aptamer Sequence:	AUGAUCAAUCGCCUCAAUUCGACAGGAGGCUCAC
The optimal secondary structure in dot-bracket notation:	..........(((((..........)))))....
The centroid secondary structure in dot-bracket notation:	..........(((((..........)))))....

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_384	Description	GL21.T
Aptamer chemistry	RNA	Length	34 nt
GC content	50.0%	Molecular weight	10,841.51 Da
Molarity of 1 μg/μl solution	92.24 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	AUGAUCAAUCGCCUCAAUUCGACAGGAGGCUCAC
Applications	Inhibits its catalytic activity. GL21.T blocked Axl-dependent transducing events in vitro, including Erk and Akt phosphorylation, cell migration and invasion, as well as in vivo lung tumor formation in mice xenografts

Type	Detail	Type	Detail
Uniprot ID	P30530	Protein name	Tyrosine-protein kinase receptor UFO (EC 2.7.10.1) (AXL oncogene)
Gene name(s)	AXL UFO	Organism	Tyrosine-protein kinase receptor UFO (EC 2.7.10.1) (AXL oncogene)
Status	reviewed in uniprot database	Length	894
Mass	98,337	Activity regulation	Activated by GAS6-binding and subsequent autophosphorylation
Involvement in disease	AXL and its ligand GAS6 are highly expressed in thyroid carcinoma tissues, and might thus be involved in thyroid tumorigenesis	Catalytic activity	Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein]
PDB ID(s)	2C5D;4RA0;5U6B;5VXZ;
Function	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation.

	Interaction ID	390
	Target type	Protein
	Target unique ID	P30530
	Activity	12 nM
	Binding Conditions/Buffer	Dulbecco's Modified Eagle's Medium (DMEM), 10% FBS, 2mM L-glutamine
	Assay	Motility of U87MG and A549 cells was analyzed by Transwell Migration Assay in the presence of GL21.T or the unrelated aptamer for 24 hours toward 10% fetal bovine serum (FBS) or Gas6 as inducers of migration
	PubMed ID	22910292

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1144	RNA	ACGAGUCUGGUCCUUCCUCCCUAAUUGCUGUUGAGGUAUCGGCUACAACAA	54.90%
Apta_138	RNA	GGAGGUGCUCCGAAAGGAACUCC	52.94%
Apta_709	RNA	AGUGAUACCAGCAUCGUCUUGAUGCCCUUGGCAGCACU	52.63%
Apta_1211	RNA	ACUAGCCUCAUCAGCUCAUGUGCCCCUCCGCCUGGAUCAC	52.50%
Apta_152	RNA	UUACCACGCGCUCUUAACUGCUAGCGCCAUGGC	50.00%
Apta_358	DNA	GGGAATCGCCACCCGACGCAGGGTTTCCC	50.00%
Apta_362	DNA	GGGAACGCACCGATCGCAGGTTTCCC	50.00%
Apta_658	RNA	CUGCGAUCAGGGGUAAAUUUCCGCGCAGGCUCCACGCCGC	50.00%
Apta_955	RNA	ACGUAUCCCUUUUCGCGUA	50.00%
Apta_1249	RNA	AAUCGGCCCAAUUUUACCUUCGGACUUCAGUAUCUAGUGUGGCUUUAAAUCAUAGC	50.00%
Apta_51	RNA	GCCUUAGUAACGUGCUUUGAUGUCGAUUCGACAGGAGGC	48.72%
Apta_86	RNA	GAAACGACUCGACAACCUCGAGCGACGUGAAUCAU	48.57%
Apta_91	RNA	GAUAACCUCGAUGCGACGUUCGGCCUCAAAAUCAA	48.57%
Apta_92	RNA	ACAACUUAAUGCGACGUUGGUAAAGCAUAUCAAAC	48.57%
Apta_94	RNA	AUGACAGGAUGCAGAGCUCCACUGUCUAGUGUUUA	48.57%
Apta_1166	RNA	GAUGUAACUGAAUGAAAUGGUGAAGGACGGGUCCAGUAGGCUGC	47.73%
Apta_365	RNA	GGACCUAUGCAGUAGCCAGUGUGGACUGGGCUGCCCCCCC	47.50%
Apta_447	RNA	ACGCUCAACGAGCCAGGAACAUCGACGUCAGCAAACGCGAGCGCAACCAGUAACACC	47.37%
Apta_378	RNA	UGCUCUUAGAAUGGGACUAAGACACGGGAC	47.06%
Apta_424	RNA	UGCUAGUAUAUCGCACGGAUUUAAUCGCCGUAGAAAAGCAUGUCAAAGCCG	47.06%

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