Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 325
Aptamer sequence: CCGCCCAAATCCCTAAGAGAAGACTGTAATGACATCAAACCAGACACACTACACACGCA
Target unique ID: P15260
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_316 | B1–4 | Protein | INGR1_HUMAN | 74.5 nM | Salmon sperm DNA (0.1 mg/mL) and BSA (1 mg/mL) | 24849390 |
Structure information of aptamer
| Aptamer Sequence: | CCGCCCAAATCCCTAAGAGAAGACTGTAATGACATCAAACCAGACACACTACACACGCA |
| The optimal secondary structure in dot-bracket notation: | ........................((((.((...((......))..)).))))...... |
| The centroid secondary structure in dot-bracket notation: | .........................(((.((....(......)...)).)))....... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_316 | Description | B1–4 |
| Aptamer chemistry | DNA | Length | 59 nt |
| GC content | 47.5% | Molecular weight | 18,241.58 Da |
| Molarity of 1 μg/μl solution | 54.82 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | CCGCCCAAATCCCTAAGAGAAGACTGTAATGACATCAAACCAGACACACTACACACGCA | ||
| Applications | Assay of intracellular IFN-c | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P15260 | Protein name | Interferon gamma receptor 1 (IFN-gamma receptor 1) (IFN-gamma-R1) (CDw119) (Interferon gamma receptor alpha-chain) (IFN-gamma-R-alpha) (CD antigen CD119) |
| Gene name(s) | IFNGR1 | Organism | Interferon gamma receptor 1 (IFN-gamma receptor 1) (IFN-gamma-R1) (CDw119) (Interferon gamma receptor alpha-chain) (IFN-gamma-R-alpha) (CD antigen CD119) |
| Status | reviewed in uniprot database | Length | 489 |
| Mass | 54,405 | Activity regulation | N/A |
| Involvement in disease | Immunodeficiency 27A (IMD27A) | Catalytic activity | N/A |
| PDB ID(s) | 1FG9;1FYH;1JRH;6E3K;6E3L; |
 |
Function | Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. |
Activity data
 |
Interaction ID | 325 |
| Target type | Protein | |
| Target unique ID | P15260 | |
| Activity | 74.5 nM | |
| Binding Conditions/Buffer | Salmon sperm DNA (0.1 mg/mL) and BSA (1 mg/mL) |
|
| Assay | Evaluation of B1–4’s affinity to IFN-c. The affinity between FITC-labeled B1–4 and IFN-c was quantitatively assayed by plotting the fluorescence against the DNA concentration |
|
| PubMed ID | 24849390 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1113 | DNA | CCGCCCAAATCCCTAAGAGCACAAACACCAAACACAACCACCCCAACCAGACACACTACACACGCA | |
| Apta_321 | DNA | AACCGCCCAAATCCCTAAGAGTCGGACTGCAACCTATGCTATCGTTGATGTCTGTCCAAGCAACACAGACACACTACACACGCACA | |
| Apta_219 | DNA | AACCGCCCAAATCCCTAAGAGTCTGCACTTGTCATTTTGTATATGTATTTGGTTTTTGGCTCTCACAGACACACTACACACGCACA | |
| Apta_790 | DNA | GGGAGACAAGAATAAACGCTCAAGAAGTGAAAATGACAGAACACAACATTCGACAGGAGGCTCACAACAGGC | |
| Apta_502 | DNA | GGGAGACAAGAATAAACGCTCAAATGGACTTTTGAATGTGGCAACAAATTCGACAGGAGGCTCACAACAGGC | |
| Apta_447 | RNA | ACGCUCAACGAGCCAGGAACAUCGACGUCAGCAAACGCGAGCGCAACCAGUAACACC | |
| Apta_873 | DNA | CCACCGAGCCTACCACATGTGACATCCCAGGACATAGCTG | |
| Apta_1214 | DNA | GTCCATTATTCTGGTAGCGTTGAACAACATTCAACACGCC | |
| Apta_536 | DNA | ATCCGTCACACCTGCTCTCGAATAGAGTACTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT | |
| Apta_1111 | DNA | GTGCGTCGCATGATCGCGATTAGTGCGACCATGTACGATTCTCACGACAACGACACTGTACTTGAGGACTGTCTCC | |
| Apta_175 | DNA | AGCAGCACAGAGGTCAGATGAGGACTGTCGATAAGTATAATGGCAAGTAGCTCGATACATCCTATGCGTGCTACCGTGAA | |
| Apta_232 | DNA | AGCAGCACAGAGGTCAGATGCTATGAGCAGATCCGATAGAAAGTTAATGCCCTAGAACATCCTATGCGTGCTACCGTGAA | |
| Apta_1074 | DNA | CTTCTGCCCGCCTCCTTCCGCCACAGTATTATGTATCACAGTGCTTCATCTAATATGAGGAGACGAGATAGGCGGACACT | |
| Apta_3 | DNA | CCTGCCTTTGGCAGCCGGGCGAAGGCAACCCGACACCGACAGC | |
| Apta_468 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAT | |
| Apta_469 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAG | |
| Apta_470 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCTCAT | |
| Apta_635 | DNA | TAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAACCCCCCA | |
| Apta_637 | DNA | TAGCCGGATCGCGTTGGCCAGATGATATAAAGGGTCAACCCCCCA | |
| Apta_639 | DNA | CTAAGCACAGGGAAACCAGCTAATGAGTTAGGCCTGTCCCCCACG |
DEV TOOL
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