Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1097
Aptamer sequence: CACATTAGGGCGGGGTACTCCTATCACGTATGGGGGCCTGT
Target unique ID: Other-44
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1012 | NB1 | Other | Activated protein C (APC) | 20.2 nM | 1X PBS | 32486960 |
Structure information of aptamer
| Aptamer Sequence: | CACATTAGGGCGGGGTACTCCTATCACGTATGGGGGCCTGT |
| The optimal secondary structure in dot-bracket notation: | .........(((((...(((((((.....)))))))))))) |
| The centroid secondary structure in dot-bracket notation: | .........(((((...(((((((.....)))))))))))) |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1012 | Description | NB1 |
| Aptamer chemistry | DNA | Length | 41 nt |
| GC content | 58.5% | Molecular weight | 12,673.12 Da |
| Molarity of 1 μg/μl solution | 78.91 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | CACATTAGGGCGGGGTACTCCTATCACGTATGGGGGCCTGT | ||
| Applications | G-NB3 inhibits the inactivation of activated cofactors V and VIII with IC50 values of 11.6 and 13.1 nM | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-44 | Name | Activated protein C (APC) |
Activity data
 |
Interaction ID | 1097 |
| Target type | Other | |
| Target unique ID | Other-44 | |
| Activity | 20.2 nM | |
| Binding Conditions/Buffer | 1X PBS |
|
| Assay | Influence of APC aptamers on the anticoagulant activity of endogenously generated APC in human plasma. Pooled normal citrated human plasma was spiked with rabbit thrombomodulin (2 U/mL) and increasing concentrations of APC aptamers. Subsequently, thrombin formation was measured using the CAT method |
|
| PubMed ID | 32486960 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1023 | DNA | CTCCTTCAACGAAGTGGGTCTCCTTCAACGAAGTGGGTCTC | |
| Apta_1040 | DNA | CAATTGGGCCCGTCCGTATGGTGGGT | |
| Apta_1095 | DNA | CCCTGAGGGGTGATGTGAATCCTAACAGTAAGGAGGAAGG | |
| Apta_148 | DNA | CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGGTGGCGTGG | |
| Apta_158 | DNA | CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTG | |
| Apta_1022 | DNA | CTCCTTCAACGAAGTGGGTTCCTTCAACGAAGTGGGTCTC | |
| Apta_344 | DNA | CACTACAGAGGTTGCGTCTGTCCCACGTTGTCATGGGGGGTTGGCCTG | |
| Apta_75 | DNA | CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG | |
| Apta_149 | DNA | CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGATGGCGTGG | |
| Apta_150 | DNA | CCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGG | |
| Apta_258 | DNA | CACTGCGGGGGTCTATACGTGAGGAAGAAGTGGGCAGGTC | |
| Apta_263 | DNA | AGAGGGGAGGGTCGGGTATCGGCGTGTTCGGGGGATCTGC | |
| Apta_339 | DNA | CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTGT | |
| Apta_578 | DNA | CACCAAGCGCAGGGAATTACATTGAAGTGTGGGATTGGCT | |
| Apta_648 | DNA | GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGC | |
| Apta_964 | DNA | CACGAGAGGGGTGAGGTGTCGTATTAGAGGGAAGCTCCAA | |
| Apta_1203 | DNA | CCCTGCGGGGCTACCCGATATGTGTCCGAGTGGTG | |
| Apta_735 | DNA | AGGCCCCCAGGCTCGGTGGATGCAAACACATGACTATGGGCCCGT | |
| Apta_79 | DNA | ATCTCGAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACTCGT | |
| Apta_654 | DNA | GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTG |
DEV TOOL
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