AptaDB: Result

Interaction ID: 145

Aptamer sequence: CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA

Target unique ID: P02741

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_142	CRP-Ugu4-3	Protein	CRP_HUMAN	6.2 pM	40 mM HEPES, pH7.5, 125 mM NaCl, 5 mM KCl, 1 mM MgCl2, 0.01% Tween 20	32294882

Aptamer Sequence:	CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA
The optimal secondary structure in dot-bracket notation:	..............((..((((...))))...))...
The centroid secondary structure in dot-bracket notation:	..............((..((((...))))...))...

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_142	Description	CRP-Ugu4-3
Aptamer chemistry	DNA	Length	37 nt
GC content	59.5%	Molecular weight	11,642.29 Da
Molarity of 1 μg/μl solution	85.89 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target recognition
Sequence	CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA
Applications	Binding to target proteins

Type	Detail	Type	Detail
Uniprot ID	P02741	Protein name	C-reactive protein [Cleaved into: C-reactive protein(1-205)]
Gene name(s)	CRP PTX1	Organism	C-reactive protein [Cleaved into: C-reactive protein(1-205)]
Status	reviewed in uniprot database	Length	224
Mass	25,039	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	1B09;1GNH;1LJ7;3L2Y;3PVN;3PVO;7PK9;7PKB;7PKD;7PKE;7PKF;7PKG;7PKH;
Function	Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine.

	Interaction ID	145
	Target type	Protein
	Target unique ID	P02741
	Activity	6.2 pM
	Binding Conditions/Buffer	40 mM HEPES, pH7.5, 125 mM NaCl, 5 mM KCl, 1 mM MgCl2, 0.01% Tween 20
	Assay	Representative SPR sensorgrams showing the interaction between the CRP and the aptamer CRP-Ugu4-3
	PubMed ID	32294882

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_642	DNA	ATCCAATACCTCAGAACTCAGTTCGAGTCGTAAAGGGGAATCGCA	57.78%
Apta_7	DNA	CCAGTCTCCCGTTTACCGCGCCTACACATGTCTGAATGCC	57.50%
Apta_283	DNA	ACTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGA	57.50%
Apta_287	DNA	CAGCACCGACCTTGTGCTTTGGGAGTGCTGGTCCAAGGGCGTTAATGGACA	56.86%
Apta_449	DNA	CGCACTATGTTTTACGAGCCGTTTCCTCGGCAGATAGTAAGTGCG	55.56%
Apta_930	DNA	TTGCACTATGTTTTAGCTAGGGTTCCCTCCGGAGATAGTAAGTGCAA	55.32%
Apta_257	DNA	GGCAGAAGAAATATCGAAACCCAGAATGGTCGGCCAGGCG	55.00%
Apta_831	DNA	CCGCCCAGCGGGGGTAGGGCCGGACGTAGGAGGAGCTGCG	55.00%
Apta_880	DNA	GCCGCACAATCGGGTTGGGCAGGGAGGGCACGTGTGGCGC	55.00%
Apta_916	DNA	GGCGTATGCAGTTTTGTAGGGTTATCAAGGCTTGACGATT	55.00%
Apta_937	DNA	TTGCCCACAGATCTGTGGAAGCCGAACCGCGTGCTAGTCGTG	54.76%
Apta_666	DNA	TCGACCTCGCGCGAGGAGGGTGGAGGGTCGTAGAGCGCGTA	53.66%
Apta_643	DNA	ACCGATCCATCGAGTTTCTGAGAAAGGCCCGGAGAAACCGCGAGA	53.33%
Apta_819	DNA	GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT	53.33%
Apta_820	DNA	TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA	53.33%
Apta_163	DNA	GCGCATGACCATTGATGTCTGCACCATCAGAGGCAGAG	52.63%
Apta_406	DNA	AAAGCCCACTGCATAGAGGCCGCTCAACATGCGAGCCGGT	52.50%
Apta_962	DNA	CCACACCGAGATGTTGTGACGGTTCCGATTGGCAAACTGA	52.50%
Apta_965	DNA	CACGGCATGTGTGGCCGGATGCCTATATCGGTCGACTGCA	52.50%
Apta_1270	DNA	GTATATCAAGCATCGCGTGTTTACACATGCGAGAGGTGAA	52.50%

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