AptaDB: Result

Interaction ID: 165

Aptamer sequence: GTGTGCAGATCTGATTGCCTGCGTCAACCTACATACAAC

Target unique ID: P04183

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_161	TK1_apta3	Protein	KITH_HUMAN	2.4 nM	PBS	30900869

Aptamer Sequence:	GTGTGCAGATCTGATTGCCTGCGTCAACCTACATACAAC
The optimal secondary structure in dot-bracket notation:	(((((.((...((((.......))))..)).)))))...
The centroid secondary structure in dot-bracket notation:	(((((.((...((((.......))))..)).)))))...

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_161	Description	TK1_apta3
Aptamer chemistry	DNA	Length	39 nt
GC content	48.7%	Molecular weight	12,166.62 Da
Molarity of 1 μg/μl solution	82.19 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	GTGTGCAGATCTGATTGCCTGCGTCAACCTACATACAAC
Applications	Diagnostic assays

Type	Detail	Type	Detail
Uniprot ID	P04183	Protein name	Thymidine kinase, cytosolic (EC 2.7.1.21)
Gene name(s)	TK1	Organism	Thymidine kinase, cytosolic (EC 2.7.1.21)
Status	reviewed in uniprot database	Length	234
Mass	25,469	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=ATP + thymidine = ADP + dTMP + H(+)
PDB ID(s)	1W4R;1XBT;2ORV;2WVJ;
Function	Cell-cycle-regulated enzyme of importance in nucleotide metabolism.

No relevant experimental diagram	Interaction ID	165
	Target type	Protein
	Target unique ID	P04183
	Activity	2.4 nM
	Binding Conditions/Buffer	PBS
	Assay	N/A
	PubMed ID	30900869

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_159	DNA	GCGTGCAGCATGACCACCGATGTCACACTTAATGAGAAT	58.97%
Apta_363	DNA	ATTGACCGCTGTGTGACGCAACACTCAAT	56.41%
Apta_818	DNA	GGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCA	55.56%
Apta_597	DNA	TACTGGGCTGGGCTTGGATTAGATCACAGTGGAACATACAGGACACACG	55.10%
Apta_1018	DNA	ATCCCTGCAGTTTGGTAGGGTGCGAGCAACATCAGGCACCTGCAGG	54.35%
Apta_497	DNA	GTTCGATTGGATTGTGCCGGAAGTGCTGGCTCGAAC	53.85%
Apta_489	DNA	GGGGTTAAATATTCCCACATTGCCTGCGCCAGTACAAATAG	53.66%
Apta_160	DNA	CGGTGCAGTGGATACATGCCAGCCGTAGCCATCGTGGATA	52.50%
Apta_473	DNA	CCCTGCACTCGCAATGGCCCCTCTACACCACAAACGGACA	52.50%
Apta_1214	DNA	GTCCATTATTCTGGTAGCGTTGAACAACATTCAACACGCC	52.50%
Apta_1043	DNA	TTCAAGGCGGCGAGATTTAGTGGTTGGGAGGCTGTACGCCCTACGTGAAC	52.00%
Apta_573	DNA	CGTACAGTGGGCTATATTGCTGACGTAAGAGCTGCTTTCAATGCATTGGCATAGATGTCA	51.67%
Apta_162	DNA	CCGTACATACTACGTACGTACGTGAACAGAGGCAGAG	51.28%
Apta_163	DNA	GCGCATGACCATTGATGTCTGCACCATCAGAGGCAGAG	51.28%
Apta_423	DNA	TGGGAGCTGATGTCGCATGGGTTTTGATCACATGA	51.28%
Apta_809	DNA	GGGTACGTATTTGGTTTATAGATAGTAA	51.28%
Apta_1083	DNA	TGCTGTTGTCACCTGCCTCGTCTCCCTCGT	51.28%
Apta_1176	DNA	AGTATGATATCGGTGTTTATGGTGTCTGTCTTCATACT	51.28%
Apta_777	DNA	GAGCCCTATCTCACACCGCACCCGCAAACTATCATCCTACATG	51.16%
Apta_835	DNA	TATGGCGTGGCAAGCTTGGCCCGCTTCTCAAGCATGGTTATCTAC	51.11%

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