Aptamer-target interaction descriptor
Aptamer-target interaction information
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1197 | HBEGFap2 | Protein | HGF_HUMAN | 270 nM | 10 mM Tris-HCl, 150 mM NaCl, and 100 mM KCl | 28627727 |
Structure information of aptamer
| Aptamer Sequence: | GGGGGCATGGGGG |
| The optimal secondary structure in dot-bracket notation: | ............. |
| The centroid secondary structure in dot-bracket notation: | ............. |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1197 | Description | HBEGFap2 |
| Aptamer chemistry | DNA | Length | 13 nt |
| GC content | 84.6% | Molecular weight | 4,136.68 Da |
| Molarity of 1 μg/μl solution | 241.74 μM | Number of G-quadruplexes | 2 |
| G-Score | 19 | Function | Target detection |
| Sequence | GGGGGCATGGGGG | ||
| Applications | Detect hepatocyte growth factor | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P14210 | Protein name | Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain] |
| Gene name(s) | HGF HPTA | Organism | Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain] |
| Status | reviewed in uniprot database | Length | 728 |
| Mass | 83,134 | Activity regulation | N/A |
| Involvement in disease | Deafness, autosomal recessive, 39 (DFNB39) | Catalytic activity | N/A |
| PDB ID(s) | 1BHT;1GMN;1GMO;1GP9;1NK1;1SHY;1SI5;2HGF;2QJ2;3HMS;3HMT;3HN4;3MKP;3SP8;4D3C;4K3J;4O3T;4O3U;5COE;5CP9;5CS1;5CS3;5CS5;5CS9;5CSQ;5CT1;5CT2;5CT3;7MO7;7MO8;7MO9;7MOA;7MOB;7OCL;7OCM; |
 |
Function | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. |
Activity data
 |
Interaction ID | 1259 |
| Target type | Protein | |
| Target unique ID | P14210 | |
| Activity | 270 nM | |
| Binding Conditions/Buffer | 10 mM Tris-HCl, 150 mM NaCl, and 100 mM KCl |
|
| Assay | SPR sensorgram of PQSs bound to HGF or HBEGF protein |
|
| PubMed ID | 28627727 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1196 | DNA | GGGGGATGGGGG | |
| Apta_211 | DNA | GGTTGGTGTGGTTGG | |
| Apta_483 | DNA | ATTGGGAGGGATTGGGTGGG | |
| Apta_672 | DNA | GGGGTGAAACGGGTCCCG | |
| Apta_390 | DNA | GGTGCGGCGGGACTAGTGGGTGTG | |
| Apta_200 | DNA | AGGAAGGCTTTAGGT | |
| Apta_1038 | DNA | CCCGTCCGTATGGTGGG | |
| Apta_544 | DNA | GCGGGCGGTTGTATAGCGG | |
| Apta_1039 | DNA | GCCCGTCCGTATGGTGGGT | |
| Apta_141 | DNA | GTTGACGGGCGATTGGTCTT | |
| Apta_198 | DNA | AGGAAGGCTTTAGGTCTGAG | |
| Apta_207 | DNA | AGGAAGGCTTTAGGTGGAAT | |
| Apta_1098 | DNA | ATGGCCAGGGGGGAGGGG | |
| Apta_13 | DNA | AGGGGGGGGGGAGGGGTCGGGGTGG | |
| Apta_14 | DNA | AGCGGGGGGGGCTGGGCTGGGTGGG | |
| Apta_22 | DNA | TGTGGGGGTGGACTGGGTGGGTACC | |
| Apta_24 | DNA | TGTGGGGATGGATGGGCCGGGCTGC | |
| Apta_231 | RNA | GGGAGGACGAUGCGGGUGUGGGGCG | |
| Apta_515 | DNA | CCTGGGGGAGTATTGCGGAGGAAGG | |
| Apta_806 | DNA | GGTTGGATGTAAGGTTGGAGGGGGG |
DEV TOOL
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