Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1019
Aptamer sequence: GATTGAGTAGATAGTGGTTCTGTACGTAGTGAAAGAGTGG
Target unique ID: Other-35
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_928 | N5 | Other | Human PD-L1 | 441 nM | 136 mM NaCl, 2.6 mM KCl, 4 mM Na2HPO4, 1.8 mM KH2PO4, 2 mM MgCl2, pH 7.4 | 33155587 |
Structure information of aptamer
| Aptamer Sequence: | GATTGAGTAGATAGTGGTTCTGTACGTAGTGAAAGAGTGG |
| The optimal secondary structure in dot-bracket notation: | .((((.((..((((.....)))))).)))).......... |
| The centroid secondary structure in dot-bracket notation: | .((((.((...(((.....))).)).)))).......... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_928 | Description | N5 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 42.5% | Molecular weight | 12,550.08 Da |
| Molarity of 1 μg/μl solution | 79.68 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | GATTGAGTAGATAGTGGTTCTGTACGTAGTGAAAGAGTGG | ||
| Applications | TNA aptamer N5 was specifically accumulated at the tumour site, and significantly inhibited tumour growth in vivo | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-35 | Name | Human PD-L1 |
Activity data
 |
Interaction ID | 1019 |
| Target type | Other | |
| Target unique ID | Other-35 | |
| Activity | 441 nM | |
| Binding Conditions/Buffer | 136 mM NaCl, 2.6 mM KCl, 4 mM Na2HPO4, 1.8 mM KH2PO4, 2 mM MgCl2, pH 7.4 |
|
| Assay | The dissociation constants of TNA aptamers N5 were around 400 nM, determined by a nitrocellulose filter binding assay (n = 3) |
|
| PubMed ID | 33155587 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_929 | DNA | GAGTGGGATGGATAGAGAATAAGTGTGGAGTGGAAGGTGA | |
| Apta_1210 | DNA | GGGGAGGCAGTGTGTTGTGTCGTGTGTGTGCTTGG | |
| Apta_987 | DNA | GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC | |
| Apta_350 | DNA | GGTGGTGGTGGTTGTGGTGGTGGTGG | |
| Apta_693 | DNA | CGAGTTGAGCCGGGCGCGGTACGGGTACTGGTATGTGTGG | |
| Apta_1247 | DNA | GGTGGTTGCTGTTGTTTGTGGTTGGTGTGC | |
| Apta_144 | DNA | GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG | |
| Apta_748 | DNA | AGCTTGAGGGTGGGCGGGTGGACGCGGTAGTGGTATATAGGTCGG | |
| Apta_930 | DNA | TTGCACTATGTTTTAGCTAGGGTTCCCTCCGGAGATAGTAAGTGCAA | |
| Apta_58 | DNA | GCACCTTGATGACATGATAGTCGTTGTGTATGCAGTTGGC | |
| Apta_75 | DNA | CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG | |
| Apta_223 | DNA | CATTGAGATAGCTAGTTGTAGCTGCGTCATAGGCTGGGTTGGGTCTAGTGGTTGGGTGTG | |
| Apta_260 | DNA | GACGTTAAGCTAGCAGGTGTTAGGTCCCGTGGTGATGAAT | |
| Apta_267 | DNA | GAGTGCGTAATGGTACGATTTGGGAAGTGGCTTGGGGTGG | |
| Apta_682 | DNA | CCTTGTAGATCGGGTGTGGTTTGGCGTAGG | |
| Apta_757 | DNA | GGTGGTGGTGGTTGTGGTGGTGG | |
| Apta_1205 | DNA | GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG | |
| Apta_1240 | DNA | GGAGTAGTGGGGATTGTGGTGTATGTTGTC | |
| Apta_571 | DNA | ACCTGCGATAGGTAGTTTGTTTCTGTTGCCCTAGCGTCCGTCGTAACAAGTCA | |
| Apta_596 | DNA | GGTGGCTGTTGGTCGAGGCTTATTAGTGTAGTCAATGGAGGCAGGCGACG |
DEV TOOL
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